EzCatDB: D00071

DB codeD00071
CATH domainDomain 13.40.50.720Catalytic domain
Domain 23.50.50.60Catalytic domain
E.C.1.18.1.2
CSA1e6e
MACiEM0142

CATH domainRelated DB codes (homologues)
3.40.50.720S00543,S00551,S00552,S00553,S00602,S00604,S00605,S00608,S00610,S00625,S00319,S00328,S00329,S00330,S00331,S00332,D00456,D00457,D00458,S00324,S00320,S00325,S00326,S00327,D00459,S00335,S00336,S00334,T00219,S00339,D00513,D00001,D00002,D00003,D00005,D00007,D00008,D00010,D00012,D00017,D00018,D00023,D00027,D00028,D00031,D00032,D00033,D00034,D00035,D00037,D00048,D00476,D00481,D00482,D00490,D00492,D00494,D00545,D00601,D00603,D00604,D00605,D00615,D00845,D00857,D00858,M00161,M00171,M00210,T00002,T00010,T00011,T00015,T00227,T00247,T00408,T00414,D00827,D00262,D00274,D00275,M00035,T00109
3.50.50.60M00163,D00015,D00041,D00042,D00045,D00064,T00004,T00015,T00017,T00025,T00211,T00213,T00233,T00242

Enzyme Name
Swiss-protKEGG

P08165
Protein nameNADPH:adrenodoxin oxidoreductase, mitochondrialferredoxin---NADP+ reductase
adrenodoxin reductase
ferredoxin-nicotinamide adenine dinucleotide phosphate reductase
ferredoxin-NADP+ reductase
TPNH-ferredoxin reductase
ferredoxin-NADP+ oxidoreductase
NADP+:ferredoxin oxidoreductase
ferredoxin-TPN reductase
reduced nicotinamide adenine dinucleotide phosphate-adrenodoxinreductase
ferredoxin-NADP+-oxidoreductase
NADPH:ferredoxin oxidoreductase
ferredoxin-nicotinamide-adenine dinucleotide phosphate (oxidized)reductase
ferredoxin---NADP+ reductase
SynonymsAdrenodoxin reductase
AR
EC 1.18.1.2
Ferredoxin--NADP(+) reductase
Ferredoxin reductase

KEGG pathways
MAP codePathways
MAP00195Photosynthesis

Swiss-prot:Accession NumberP08165
Entry nameADRO_BOVIN
Activity2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH.
SubunitMonomer. Interacts directly with FDX1.
Subcellular locationMitochondrion matrix.
CofactorFAD.


CofactorsSubstratesProducts
KEGG-idC00176C00138C00006C00139C00005
CompoundFlavinReduced ferredoxinNADP+Oxidized ferredoxinNADPH
Typeamide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms)heavy metal,peptide/protein,sulfide groupamide group,amine group,nucleotideheavy metal,peptide/protein,sulfide groupamide group,amine group,nucleotide
1cjcA01Bound:FADUnboundUnboundUnboundUnbound
1e1lA01Bound:FADUnboundUnboundUnboundUnbound
1e1kA01Bound:FADUnboundUnboundUnboundUnbound
1e1mA01Bound:FADUnboundUnboundUnboundUnbound
1e1nA01Bound:FADUnboundUnboundUnboundUnbound
1e6eA01Bound:FADBound:FES(chain B)UnboundUnboundUnbound
1e6eC01Bound:FADBound:FES(chain D)UnboundUnboundUnbound
1cjcA02UnboundUnboundUnboundUnboundUnbound
1e1lA02UnboundUnboundBound:NAPUnboundUnbound
1e1kA02UnboundUnboundBound:NAPUnboundUnbound
1e1mA02UnboundUnboundBound:NAPUnboundUnbound
1e1nA02UnboundUnboundUnboundUnboundUnbound
1e6eA02UnboundUnboundUnboundUnboundUnbound
1e6eC02UnboundUnboundUnboundUnboundUnbound

Active-site residues
resource
literature [6]
pdbCatalytic residues
1cjcA01
1e1lA01
1e1kA01
1e1mA01
1e1nA01
1e6eA01
1e6eC01
1cjcA02ARG 124;TYR 331
1e1lA02ARG 124;TYR 331
1e1kA02ARG 124;TYR 331
1e1mA02ARG 124;TYR 331
1e1nA02ARG 124;TYR 331
1e6eA02ARG 124;TYR 331
1e6eC02ARG 124;TYR 331

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[6]Fig.7, p.10991-109923
[7]p.598-599
[8]p.29-31
[10]p.2789

references
[1]
PubMed ID8230204
JournalJ Mol Biol
Year1993
Volume234
Pages245-8
AuthorsKuban RJ, Marg A, Resch M, Ruckpaul K
TitleCrystallization of bovine adrenodoxin reductase in a new unit cell and its crystallographic characterization.
[2]
PubMed ID9188745
JournalProteins
Year1997
Volume28
Pages289-92
AuthorsLapko A, Muller A, Heese O, Ruckpaul K, Heinemann U
TitlePreparation and crystallization of a cross-linked complex of bovine adrenodoxin and adrenodoxin reductase.
[3]
PubMed ID9551550
JournalStructure
Year1998
Volume6
Pages269-80
AuthorsMuller A, Muller JJ, Muller YA, Uhlmann H, Bernhardt R, Heinemann U
TitleNew aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108).
[4]
PubMed ID9989598
JournalFEBS Lett
Year1999
Volume443
Pages167-9
AuthorsVonrhein C, Schmidt U, Ziegler GA, Schweiger S, Hanukoglu I, Schulz GE
TitleChaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli.
[5]
CommentsX-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 33-492
Medline ID99299392
PubMed ID10369776
JournalJ Mol Biol
Year1999
Volume289
Pages981-90
AuthorsZiegler GA, Vonrhein C, Hanukoglu I, Schulz GE
TitleThe structure of adrenodoxin reductase of mitochondrial P450 systems: electron transfer for steroid biosynthesis.
Related Swiss-protP08165
[6]
CommentsX-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS)
Medline ID20455764
PubMed ID10998235
JournalBiochemistry
Year2000
Volume39
Pages10986-95
AuthorsZiegler GA, Schulz GE
TitleCrystal structures of adrenodoxin reductase in complex with NADP+ and NADPH suggesting a mechanism for the electron transfer of an enzyme family.
Related PDB1e1k,1e1m,1e1n
Related Swiss-protP08165
[7]
PubMed ID10899784
JournalProteins
Year2000
Volume40
Pages590-612
AuthorsGrinberg AV, Hannemann F, Schiffler B, Muller J, Heinemann U, Bernhardt R
TitleAdrenodoxin: structure, stability, and electron transfer properties.
[8]
PubMed ID11716458
JournalArch Biochem Biophys
Year2001
Volume396
Pages25-34
AuthorsGrinberg AV, Bernhardt R
TitleContribution of a salt bridge to the thermostability of adrenodoxin determined by site-directed mutagenesis.
[9]
PubMed ID11298752
JournalEur J Biochem
Year2001
Volume268
Pages2338-43
AuthorsTuckey RC, McKinley AJ, Headlam MJ
TitleOxidized adrenodoxin acts as a competitive inhibitor of cytochrome P450scc in mitochondria from the human placenta.
[10]
CommentsX-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF COMPLEX WITH ADRENODOXIN
Medline ID21264735
PubMed ID11053423
JournalJ Biol Chem
Year2001
Volume276
Pages2786-9
AuthorsMuller JJ, Lapko A, Bourenkov G, Ruckpaul K, Heinemann U
TitleAdrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis.
Related PDB1e6e
Related Swiss-protP08165
[11]
PubMed ID12137805
JournalJ Steroid Biochem Mol Biol
Year2002
Volume81
Pages153-8
AuthorsTuckey RC, Headlam MJ
TitlePlacental cytochrome P450scc (CYP11A1): comparison of catalytic properties between conditions of limiting and saturating adrenodoxin reductase.
[12]
PubMed ID12071965
JournalEur J Biochem
Year2002
Volume269
Pages3005-13
AuthorsFischer F, Raimondi D, Aliverti A, Zanetti G
TitleMycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase.


createdupdated
2004-03-242009-02-26
Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2010)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)

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