EzCatDB: D00144

DB codeD00144
RLCP classification1.19.30000.10
CATH domainDomain 13.40.50.2300
Domain 23.40.50.180Catalytic domain
E.C.3.1.1.61
CSA1chd

CATH domainRelated DB codes (homologues)
3.40.50.2300M00143

Enzyme Name
Swiss-protKEGG

P04042
Protein nameChemotaxis response regulator protein-glutamate methylesteraseprotein-glutamate methylesterase
chemotaxis-specific methylesterase
methyl-accepting chemotaxis protein methyl-esterase
CheB methylesterase
methylesterase CheB
protein methyl-esterase
protein carboxyl methylesterase
PME
protein methylesterase
protein-L-glutamate-5-O-methyl-ester acylhydrolase
SynonymsEC 3.1.1.61


Swiss-prot:Accession NumberP04042
Entry nameCHEB_SALTY
ActivityProtein L-glutamate O(5)-methyl ester + H(2)O = protein L-glutamate + methanol.
Subunit
Subcellular locationCytoplasm.
Cofactor


SubstratesProducts
KEGG-idC04142C00001C00614C00132
CompoundProtein glutamate methyl esterH2OProtein glutamateMethanol
Typepeptide/proteinH2Ocarboxyl group,peptide/proteincarbohydrate
1a2oA01Unbound
UnboundUnbound
1a2oB01Unbound
UnboundUnbound
1a2oA02Unbound
UnboundUnbound
1a2oB02Unbound
UnboundUnbound
1chdAUnbound
UnboundUnbound

Active-site residues
resource
PDB;1a2o & Swiss-prot;P04042
pdbCatalytic residuesModified residuesMain-chain involved in catalysis
1a2oA01
ASP 56(phosphorylation)

1a2oB01
ASP 56(phosphorylation)

1a2oA02SER 164;HIS 190;ASP 286

THR 165;MET 283(oxyanion hole)
1a2oB02SER 164;HIS 190;ASP 286

THR 165;MET 283(oxyanion hole)
1chdASER 164;HIS 190;ASP 286

THR 165;MET 283(oxyanion hole)

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[2]Fig.9, p.279-282

references
[1]
CommentsPHOSPHORYLATION.
Medline ID88165060
PubMed ID3280143
JournalCell
Year1988
Volume53
Pages79-87
AuthorsHess JF, Oosawa K, Kaplan N, Simon MI
TitlePhosphorylation of three proteins in the signaling pathway of bacterial chemotaxis.
Related Swiss-protP04042
[2]
CommentsX-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 152-349.
Medline ID95333182
PubMed ID7608974
JournalJ Mol Biol
Year1995
Volume250
Pages276-90
AuthorsWest AH, Martinez-Hackert E, Stock AM
TitleCrystal structure of the catalytic domain of the chemotaxis receptor methylesterase, CheB.
Related PDB1chd
Related Swiss-protP04042
[3]
PubMed ID7567955
JournalProteins
Year1995
Volume21
Pages345-50
AuthorsWest AH, Djordjevic S, Martinez-Hackert E, Stock AM
TitlePurification, crystallization, and preliminary X-ray diffraction analyses of the bacterial chemotaxis receptor modifying enzymes.
[4]
PubMed ID9139915
JournalJ Bacteriol
Year1997
Volume179
Pages2963-8
AuthorsBrooun A, Zhang W, Alam M
TitlePrimary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinarium.
[5]
CommentsCHARACTERIZATION.
Medline ID98434376
PubMed ID9760239
JournalBiochemistry
Year1998
Volume37
Pages14038-47
AuthorsAnand GS, Goudreau PN, Stock AM
TitleActivation of methylesterase CheB: evidence of a dual role for the regulatory domain.
Related Swiss-protP04042
[6]
PubMed ID10049806
JournalJ Struct Biol
Year1998
Volume124
Pages189-200
AuthorsDjordjevic S, Stock AM
TitleStructural analysis of bacterial chemotaxis proteins: components of a dynamic signaling system.
[7]
CommentsX-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
Medline ID98132598
PubMed ID9465023
JournalProc Natl Acad Sci U S A
Year1998
Volume95
Pages1381-6
AuthorsDjordjevic S, Goudreau PN, Xu Q, Stock AM, West AH
TitleStructural basis for methylesterase CheB regulation by a phosphorylation-activated domain.
Related PDB1a2o
Related Swiss-protP04042
[8]
PubMed ID10231491
JournalMol Microbiol
Year1999
Volume32
Pages357-65
AuthorsNishiyama SI, Umemura T, Nara T, Homma M, Kawagishi I
TitleConversion of a bacterial warm sensor to a cold sensor by methylation of a single residue in the presence of an attractant.
[9]
PubMed ID10760170
JournalMol Microbiol
Year2000
Volume36
Pages132-40
AuthorsShiomi D, Okumura H, Homma M, Kawagishi I
TitleThe aspartate chemoreceptor Tar is effectively methylated by binding to the methyltransferase mainly through hydrophobic interaction.
[10]
PubMed ID11435446
JournalJ Biol Chem
Year2001
Volume276
Pages32984-9
AuthorsBarnakov AN, Barnakova LA, Hazelbauer GL
TitleLocation of the receptor-interaction site on CheB, the methylesterase response regulator of bacterial chemotaxis.
[11]
PubMed ID11243808
JournalJ Mol Biol
Year2001
Volume307
Pages119-35
AuthorsKim C, Jackson M, Lux R, Khan S
TitleDeterminants of chemotactic signal amplification in Escherichia coli.
[12]
PubMed ID12022879
JournalBiochemistry
Year2002
Volume41
Pages6752-60
AuthorsAnand GS, Stock AM
TitleKinetic basis for the stimulatory effect of phosphorylation on the methylesterase activity of CheB.
[13]
PubMed ID12123464
JournalMol Microbiol
Year2002
Volume45
Pages555-68
AuthorsZimmer MA, Szurmant H, Saulmon MM, Collins MA, Bant JS, Ordal GW
TitleThe role of heterologous receptors in McpB-mediated signalling in Bacillus subtilis chemotaxis.


createdupdated
2004-03-252009-02-26
Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2010)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)

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