|
|
| CATH domain | Related DB codes (homologues) |
|---|
| 2.40.10.10 | M00139,D00214,M00167,D00426,M00133,D00428,D00429,D00430,D00431,D00432,D00433,D00434,D00435,M00227,M00209,D00194,D00211,D00212,D00216,M00212,D00224,D00497,M00217,M00216,D00528,D00848,D00850,D00851,D00852,D00855,M00152,M00155,M00157,M00181,M00315,M00316,M00317,M00348,M00349,T00074,T00410,T00411 |
| Enzyme Name | | Swiss-prot | KEGG |
|---|
| P00775 | P35049 | P35031 | P35033 | P00762 | P00763 | P00761 | P00760 | P07477 |
|---|
| Protein name | Trypsin | Trypsin | Trypsin-1 | Trypsin-3 | Anionic trypsin-1 | Anionic trypsin-2 | Trypsin | Cationic trypsin | Trypsin-1 | trypsinalpha-trypsinbeta-trypsincocoonaseparenzymeparenzymoltryptartrypurepseudotrypsintryptasetripcellimsperm receptor hydrolase |
|---|
| Synonyms | EC 3.4.21.4SGT | EC 3.4.21.4 | EC 3.4.21.4Trypsin I | EC 3.4.21.4Trypsin III | EC 3.4.21.4Anionic trypsin IPretrypsinogen ISerine protease 1 | EC 3.4.21.4Anionic trypsin IIPretrypsinogen IISerine protease 2 | EC 3.4.21.4 | EC 3.4.21.4Beta-trypsin | EC 3.4.21.4Trypsin ICationic trypsinogenSerine protease 1Beta-trypsin |
|---|
| Contains | None | None | None | None | None | None | None | Alpha-trypsin chain 1Alpha-trypsin chain 2 | Alpha-trypsin chain 1Alpha-trypsin chain 2 |
|---|
| Swiss-prot:Accession Number | P00775 | P35049 | P35031 | P35033 | P00762 | P00763 | P00761 | P00760 | P07477 |
|---|
| Entry name | TRYP_STRGR | TRYP_FUSOX | TRY1_SALSA | TRY3_SALSA | TRY1_RAT | TRY2_RAT | TRYP_PIG | TRY1_BOVIN | TRY1_HUMAN |
|---|
| Activity | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. |
|---|
| Subunit |
|
|
|
|
|
|
|
|
|
|---|
| Subcellular location |
| Secreted. | Secreted, extracellular space. | Secreted, extracellular space. | Secreted, extracellular space. | Secreted, extracellular space. | Secreted, extracellular space. | Secreted, extracellular space. | Secreted, extracellular space. |
|---|
| Cofactor |
|
| Binds 1 calcium ion per subunit. | Binds 1 calcium ion per subunit (By similarity). | Binds 1 calcium ion per subunit. | Binds 1 calcium ion per subunit. | Binds 1 calcium ion per subunit. | Binds 1 calcium ion per subunit. | Binds 1 calcium ion per subunit. |
|---|
| References for Catalytic Mechanism | | References | Sections | No. of steps in catalysis |
|---|
| [10] | p.905 |
| | [47] | Fig.9 |
| | [53] | Fig. 2, p.338-340 | 3 | | [68] | p.17079 |
| | [73] | p.355-356 |
| | [78] | p.146 |
| | [129] | Fig.2, p.43362 | 4 |
| references | | [1] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF CALCIUM-BINDING SITE. |
|---|
| Medline ID | 76072097 |
|---|
| PubMed ID | 512 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1975 |
|---|
| Volume | 98 |
|---|
| Pages | 693-717 |
|---|
| Authors | Bode W, Schwager P |
|---|
| Title | The refined crystal structure of bovine beta-trypsin at 1.8 A resolution. II. Crystallographic refinement, calcium binding site, benzamidine binding site and active site at pH 7.0. |
|---|
| Related Swiss-prot | P00760 |
|---|
| [2] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). |
|---|
| Medline ID | 77112431 |
|---|
| PubMed ID | 556951 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1977 |
|---|
| Volume | 16 |
|---|
| Pages | 654-64 |
|---|
| Authors | Kossiakoff AA, Chambers JL, Kay LM, Stroud RM |
|---|
| Title | Structure of bovine trypsinogen at 1.9 A resolution. |
|---|
| Related PDB | 1tgn |
|---|
| Related Swiss-prot | P00760 |
|---|
| [3] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 864704 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1977 |
|---|
| Volume | 111 |
|---|
| Pages | 415-38 |
|---|
| Authors | Fehlhammer H, Bode W, Huber R |
|---|
| Title | Crystal structure of bovine trypsinogen at 1-8 A resolution. II. Crystallographic refinement, refined crystal structure and comparison with bovine trypsin. |
|---|
| Related PDB | 1tgb |
|---|
| [4] |
|---|
| Comments | X-ray crystallography |
|---|
| Journal | Acta Crystallogr B |
|---|
| Year | 1982 |
|---|
| Volume | 38 |
|---|
| Pages | 1462-72 |
|---|
| Authors | Walter J, Steigemann W, Singh TP, Bartunik H, Bode W, Huber R |
|---|
| Title | On the disordered activation domain in trypsinogen: chemical labelling and low-temperature crystallography. |
|---|
| Related PDB | 1tgc,1tgt,2ptn,2tga,2tgt,2tpi,3ptn |
|---|
| [5] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 7169635 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1982 |
|---|
| Volume | 162 |
|---|
| Pages | 839-68 |
|---|
| Authors | Bolognesi M, Gatti G, Menagatti E, Guarneri M, Marquart M, Papamokos E, Huber R |
|---|
| Title | Three-dimensional structure of the complex between pancreatic secretory trypsin inhibitor (Kazal type) and trypsinogen at 1.8 A resolution. Structure solution, crystallographic refinement and preliminary structural interpretation. |
|---|
| Related PDB | 1tgs |
|---|
| [6] |
|---|
| Comments | X-ray crystallography |
|---|
| Journal | Acta Crystallogr B |
|---|
| Year | 1983 |
|---|
| Volume | 39 |
|---|
| Pages | 480-90 |
|---|
| Authors | Marquart M, Walter J, Deisenhofer J, Bode W, Huber R |
|---|
| Title | The geometry of the reactive site and of the peptide groups in trypsin, trypsinogen and its complexes with inhibitors. |
|---|
| Related PDB | 1tpa,1tpo,1tpp,2ptc,2tgp,3ptb,3tpi,1tpo,1tpp,2ptc,2tgp,3ptb,3tpi |
|---|
| [7] |
|---|
| Comments | Neutron Diffraction |
|---|
| PubMed ID | 6712567 |
|---|
| Journal | Basic Life Sci |
|---|
| Year | 1984 |
|---|
| Volume | 27 |
|---|
| Pages | 281-304 |
|---|
| Authors | Kossiakoff AA |
|---|
| Title | Use of the neutron diffraction--H/D exchange technique to determine the conformational dynamics of trypsin. |
|---|
| Related PDB | 1ntp |
|---|
| [8] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 6207021 |
|---|
| Journal | Eur J Biochem |
|---|
| Year | 1984 |
|---|
| Volume | 144 |
|---|
| Pages | 185-90 |
|---|
| Authors | Bode W, Walter J, Huber R, Wenzel HR, Tschesche H |
|---|
| Title | The refined 2.2-A (0.22-nm) X-ray crystal structure of the ternary complex formed by bovine trypsinogen, valine-valine and the Arg15 analogue of bovine pancreatic trypsin inhibitor. |
|---|
| Related PDB | 4tpi |
|---|
| [9] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 3032921 |
|---|
| Journal | J Biochem (Tokyo) |
|---|
| Year | 1986 |
|---|
| Volume | 100 |
|---|
| Pages | 1637-46 |
|---|
| Authors | Tsunogae Y, Tanaka I, Yamane T, Kikkawa J, Ashida T, Ishikawa C, Watanabe K, Nakamura S, Takahashi K |
|---|
| Title | Structure of the trypsin-binding domain of Bowman-Birk type protease inhibitor and its interaction with trypsin. |
|---|
| Related PDB | 1tab |
|---|
| [10] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). |
|---|
| Medline ID | 87292123 |
|---|
| PubMed ID | 3112942 |
|---|
| Journal | Science |
|---|
| Year | 1987 |
|---|
| Volume | 237 |
|---|
| Pages | 905-9 |
|---|
| Authors | Sprang S, Standing T, Fletterick RJ, Stroud RM, Finer-Moore J, Xuong NH, Hamlin R, Rutter WJ, Craik CS |
|---|
| Title | The three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis. |
|---|
| Related PDB | 1trm,2trm |
|---|
| Related Swiss-prot | P00762 |
|---|
| [11] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS). |
|---|
| Medline ID | 88286735 |
|---|
| PubMed ID | 3135412 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1988 |
|---|
| Volume | 200 |
|---|
| Pages | 523-51 |
|---|
| Authors | Read RJ, James MN |
|---|
| Title | Refined crystal structure of Streptomyces griseus trypsin at 1.7 A resolution. |
|---|
| Related PDB | 1sgt |
|---|
| Related Swiss-prot | P00775 |
|---|
| [12] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 2914611 |
|---|
| Journal | FEBS Lett |
|---|
| Year | 1989 |
|---|
| Volume | 242 |
|---|
| Pages | 285-92 |
|---|
| Authors | Bode W, Greyling HJ, Huber R, Otlewski J, Wilusz T |
|---|
| Title | The refined 2.0 A X-ray crystal structure of the complex formed between bovine beta-trypsin and CMTI-I, a trypsin inhibitor from squash seeds (Cucurbita maxima). Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes. |
|---|
| Related PDB | 1ppe |
|---|
| [13] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 2614845 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1989 |
|---|
| Volume | 210 |
|---|
| Pages | 813-28 |
|---|
| Authors | Bartunik HD, Summers LJ, Bartsch HH |
|---|
| Title | Crystal structure of bovine beta-trypsin at 1.5 A resolution in a crystal form with low molecular packing density. Active site geometry, ion pairs and solvent structure. |
|---|
| Related PDB | 1tld |
|---|
| [14] |
|---|
| PubMed ID | 2125487 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1990 |
|---|
| Volume | 29 |
|---|
| Pages | 10018-22 |
|---|
| Authors | Hynes TR, Randal M, Kennedy LA, Eigenbrot C, Kossiakoff AA |
|---|
| Title | X-ray crystal structure of the protease inhibitor domain of Alzheimer's amyloid beta-protein precursor. |
|---|
| [15] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 2252895 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1990 |
|---|
| Volume | 29 |
|---|
| Pages | 8351-7 |
|---|
| Authors | Mangel WF, Singer PT, Cyr DM, Umland TC, Toledo DL, Stroud RM, Pflugrath JW, Sweet RM |
|---|
| Title | Structure of an acyl-enzyme intermediate during catalysis: (guanidinobenzoyl)trypsin. |
|---|
| Related PDB | 1gbt |
|---|
| [16] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 2226434 |
|---|
| Journal | Eur J Biochem |
|---|
| Year | 1990 |
|---|
| Volume | 193 |
|---|
| Pages | 175-82 |
|---|
| Authors | Bode W, Turk D, Sturzebecher J |
|---|
| Title | Geometry of binding of the benzamidine- and arginine-based inhibitors N alpha-(2-naphthyl-sulphonyl-glycyl)-DL-p-amidinophenylalanyl-pipe ridine (NAPAP) and (2R,4R)-4-methyl-1-[N alpha-(3-methyl-1,2,3,4-tetrahydro-8- quinolinesulphonyl)-L-arginyl]-2-piperidine carboxylic acid (MQPA) to human alpha-thrombin. X-ray crystallographic determination of the NAPAP-trypsin complex and modeling of NAPAP-thrombin and MQPA-thrombin. |
|---|
| Related PDB | 1ppc |
|---|
| [17] |
|---|
| PubMed ID | 1988014 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1991 |
|---|
| Volume | 30 |
|---|
| Pages | 133-43 |
|---|
| Authors | Prestrelski SJ, Byler DM, Liebman MN |
|---|
| Title | Comparison of various molecular forms of bovine trypsin: correlation of infrared spectra with X-ray crystal structures. |
|---|
| [18] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 1879520 |
|---|
| Journal | FEBS Lett |
|---|
| Year | 1991 |
|---|
| Volume | 287 |
|---|
| Pages | 133-8 |
|---|
| Authors | Turk D, Sturzebecher J, Bode W |
|---|
| Title | Geometry of binding of the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino phenylalanine to thrombin and trypsin. X-ray crystal structures of their trypsin complexes and modeling of their thrombin complexes. |
|---|
| Related PDB | 1pph |
|---|
| [19] |
|---|
| PubMed ID | 1794983 |
|---|
| Journal | J Biochem (Tokyo) |
|---|
| Year | 1991 |
|---|
| Volume | 110 |
|---|
| Pages | 945-50 |
|---|
| Authors | Yamane T, Kobuke M, Tsutsui H, Toida T, Suzuki A, Ashida T, Kawata Y, Sakiyama F |
|---|
| Title | Crystal structure of Streptomyces erythraeus trypsin at 2.7 A resolution. |
|---|
| [20] |
|---|
| PubMed ID | 1714504 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1991 |
|---|
| Volume | 220 |
|---|
| Pages | 757-70 |
|---|
| Authors | Housset D, Kim KS, Fuchs J, Woodward C, Wlodawer A |
|---|
| Title | Crystal structure of a Y35G mutant of bovine pancreatic trypsin inhibitor. |
|---|
| [21] |
|---|
| PubMed ID | 1904942 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1991 |
|---|
| Volume | 219 |
|---|
| Pages | 511-23 |
|---|
| Authors | Wilke ME, Higaki JN, Craik CS, Fletterick RJ |
|---|
| Title | Crystal structure of rat trypsin-S195C at -150 degrees C. Analysis of low activity of recombinant and semisynthetic thiol proteases. |
|---|
| [22] |
|---|
| PubMed ID | 2051486 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1991 |
|---|
| Volume | 219 |
|---|
| Pages | 525-32 |
|---|
| Authors | Wilke ME, Higaki JN, Craik CS, Fletterick RJ |
|---|
| Title | Crystallographic analysis of trypsin-G226A. A specificity pocket mutant of rat trypsin with altered binding and catalysis. |
|---|
| [23] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.59 ANGSTROMS). |
|---|
| Medline ID | 91351998 |
|---|
| PubMed ID | 1881877 |
|---|
| Journal | Proteins |
|---|
| Year | 1991 |
|---|
| Volume | 10 |
|---|
| Pages | 171-87 |
|---|
| Authors | Earnest T, Fauman E, Craik CS, Stroud R |
|---|
| Title | 1.59 A structure of trypsin at 120 K: comparison of low temperature and room temperature structures. |
|---|
| Related PDB | 1dpo |
|---|
| Related Swiss-prot | P00763 |
|---|
| [24] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 1554694 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1992 |
|---|
| Volume | 31 |
|---|
| Pages | 3059-64 |
|---|
| Authors | McGrath ME, Vasquez JR, Craik CS, Yang AS, Honig B, Fletterick RJ |
|---|
| Title | Perturbing the polar environment of Asp102 in trypsin: consequences of replacing conserved Ser214. |
|---|
| Related PDB | 1anb,1anc |
|---|
| [25] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). |
|---|
| Medline ID | 92201369 |
|---|
| PubMed ID | 1551419 |
|---|
| Journal | FEBS Lett |
|---|
| Year | 1992 |
|---|
| Volume | 297 |
|---|
| Pages | 143-6 |
|---|
| Authors | Huang Q, Liu S, Tang Y, Zeng F, Qian R |
|---|
| Title | Amino acid sequencing of a trypsin inhibitor by refined 1.6 A X-ray crystal structure of its complex with porcine beta-trypsin. |
|---|
| Related Swiss-prot | P00761 |
|---|
| [26] |
|---|
| PubMed ID | 1383552 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1992 |
|---|
| Volume | 227 |
|---|
| Pages | 757-75 |
|---|
| Authors | Berndt KD, Guntert P, Orbons LP, Wuthrich K |
|---|
| Title | Determination of a high-quality nuclear magnetic resonance solution structure of the bovine pancreatic trypsin inhibitor and comparison with three crystal structures. |
|---|
| [27] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 1584773 |
|---|
| Journal | Proc Natl Acad Sci U S A |
|---|
| Year | 1992 |
|---|
| Volume | 89 |
|---|
| Pages | 4407-11 |
|---|
| Authors | Takeuchi Y, Nonaka T, Nakamura KT, Kojima S, Miura K, Mitsui Y |
|---|
| Title | Crystal structure of an engineered subtilisin inhibitor complexed with bovine trypsin. |
|---|
| Related PDB | 2tld |
|---|
| [28] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 1557349 |
|---|
| Journal | Proteins |
|---|
| Year | 1992 |
|---|
| Volume | 12 |
|---|
| Pages | 203-22 |
|---|
| Authors | Finer-Moore JS, Kossiakoff AA, Hurley JH, Earnest T, Stroud RM |
|---|
| Title | Solvent structure in crystals of trypsin determined by X-ray and neutron diffraction. |
|---|
| Related PDB | 4ptp,5ptp |
|---|
| [29] |
|---|
| PubMed ID | 1557350 |
|---|
| Journal | Proteins |
|---|
| Year | 1992 |
|---|
| Volume | 12 |
|---|
| Pages | 223-36 |
|---|
| Authors | Kossiakoff AA, Sintchak MD, Shpungin J, Presta LG |
|---|
| Title | Analysis of solvent structure in proteins using neutron D2O-H2O solvent maps: pattern of primary and secondary hydration of trypsin. |
|---|
| [30] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS). |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 1993 |
|---|
| Volume | 49 |
|---|
| Pages | 318-30 |
|---|
| Authors | Smalas AO, Hordvik A |
|---|
| Title | Structure determination and refinement of benzamidine-inhibited trypsin from the North Atlantic salmon (Salmo salar) at 1.82-A resolution. |
|---|
| Related Swiss-prot | P35031 |
|---|
| [31] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 8448149 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1993 |
|---|
| Volume | 32 |
|---|
| Pages | 1914-9 |
|---|
| Authors | McGrath ME, Haymore BL, Summers NL, Craik CS, Fletterick RJ |
|---|
| Title | Structure of an engineered, metal-actuated switch in trypsin. |
|---|
| Related PDB | 1and |
|---|
| [32] |
|---|
| PubMed ID | 8444191 |
|---|
| Journal | Eur J Biochem |
|---|
| Year | 1993 |
|---|
| Volume | 212 |
|---|
| Pages | 549-55 |
|---|
| Authors | Lin G, Bode W, Huber R, Chi C, Engh RA |
|---|
| Title | The 0.25-nm X-ray structure of the Bowman-Birk-type inhibitor from mung bean in ternary complex with porcine trypsin. |
|---|
| [33] |
|---|
| Comments | X-ray crystallography |
|---|
| Journal | J Am Chem Soc |
|---|
| Year | 1993 |
|---|
| Volume | 115 |
|---|
| Pages | 12619-20 |
|---|
| Authors | Lee AY, Hagihara M, Karmacharya R, Albers MW, Schreiber SL, Clardy J |
|---|
| Title | Atomic structure of the trypsin-cyclotheonamide A complex: lessons for the design of serine protease inhibitors. |
|---|
| Related PDB | 1tyn |
|---|
| [34] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). |
|---|
| Medline ID | 93187998 |
|---|
| PubMed ID | 8445634 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1993 |
|---|
| Volume | 229 |
|---|
| Pages | 1022-36 |
|---|
| Authors | Huang Q, Liu S, Tang Y |
|---|
| Title | Refined 1.6 A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex. |
|---|
| Related PDB | 1mct |
|---|
| Related Swiss-prot | P00761 |
|---|
| [35] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 7683059 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1993 |
|---|
| Volume | 230 |
|---|
| Pages | 919-33 |
|---|
| Authors | Perona JJ, Tsu CA, Craik CS, Fletterick RJ |
|---|
| Title | Crystal structures of rat anionic trypsin complexed with the protein inhibitors APPI and BPTI. |
|---|
| Related PDB | 1brb |
|---|
| [36] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 8478942 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1993 |
|---|
| Volume | 230 |
|---|
| Pages | 934-49 |
|---|
| Authors | Perona JJ, Tsu CA, McGrath ME, Craik CS, Fletterick RJ |
|---|
| Title | Relocating a negative charge in the binding pocket of trypsin. |
|---|
| Related PDB | 1bra,1brc |
|---|
| [37] |
|---|
| PubMed ID | 8332590 |
|---|
| Journal | Protein Eng |
|---|
| Year | 1993 |
|---|
| Volume | 6 |
|---|
| Pages | 341-8 |
|---|
| Authors | Rypniewski WR, Hastrup S, Betzel C, Dauter M, Dauter Z, Papendorf G, Branner S, Wilson KS |
|---|
| Title | The sequence and X-ray structure of the trypsin from Fusarium oxysporum. |
|---|
| [38] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS). |
|---|
| Medline ID | 95035057 |
|---|
| PubMed ID | 7947985 |
|---|
| Journal | Biochim Biophys Acta |
|---|
| Year | 1994 |
|---|
| Volume | 1209 |
|---|
| Pages | 77-82 |
|---|
| Authors | Huang Q, Wang Z, Li Y, Liu S, Tang Y |
|---|
| Title | Refined 1.8 A resolution crystal structure of the porcine epsilon-trypsin. |
|---|
| Related PDB | 1ept |
|---|
| Related Swiss-prot | P00761 |
|---|
| [39] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9383379 |
|---|
| Journal | Chem Biol |
|---|
| Year | 1994 |
|---|
| Volume | 1 |
|---|
| Pages | 113-7 |
|---|
| Authors | Lee AY, Smitka TA, Bonjouklian R, Clardy J |
|---|
| Title | Atomic structure of the trypsin-A90720A complex: a unified approach to structure and function. |
|---|
| Related PDB | 1tps |
|---|
| [40] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 7798176 |
|---|
| Journal | J Biochem (Tokyo) |
|---|
| Year | 1994 |
|---|
| Volume | 116 |
|---|
| Pages | 18-25 |
|---|
| Authors | Li Y, Huang Q, Zhang S, Liu S, Chi C, Tang Y |
|---|
| Title | Studies on an artificial trypsin inhibitor peptide derived from the mung bean trypsin inhibitor: chemical synthesis, refolding, and crystallographic analysis of its complex with trypsin. |
|---|
| Related PDB | 1g9i,1smf |
|---|
| [41] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 7634078 |
|---|
| Journal | Nat Struct Biol |
|---|
| Year | 1994 |
|---|
| Volume | 1 |
|---|
| Pages | 735-43 |
|---|
| Authors | Kurinov IV, Harrison RW |
|---|
| Title | Prediction of new serine proteinase inhibitors. |
|---|
| Related PDB | 1tng,1tnh,1tni,1tnj,1tnk,1tnl |
|---|
| [42] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS). |
|---|
| Medline ID | 95148588 |
|---|
| PubMed ID | 7846025 |
|---|
| Journal | Proteins |
|---|
| Year | 1994 |
|---|
| Volume | 20 |
|---|
| Pages | 149-66 |
|---|
| Authors | Smalas AO, Heimstad ES, Hordvik A, Willassen NP, Male R |
|---|
| Title | Cold adaption of enzymes: structural comparison between salmon and bovine trypsins. |
|---|
| Related PDB | 1tbs,2tbs |
|---|
| Related Swiss-prot | P35031 |
|---|
| [43] |
|---|
| Comments | X-ray crystallography |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 1995 |
|---|
| Volume | 51 |
|---|
| Pages | 725-30 |
|---|
| Authors | Berglund GI, Smalas AO, Hordvik A, Willassen NP |
|---|
| Title | Structure of anionic salmon trypsin in a second crystal form |
|---|
| Related PDB | 1bit |
|---|
| [44] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS). |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 1995 |
|---|
| Volume | 51 |
|---|
| Pages | 73-84 |
|---|
| Authors | Rypniewski WR, Dambmann C, von der Osten C, Dauter M, Wilson K.S |
|---|
| Title | Structure of inhibited trypsin from Fusarium oxysporum at 1.55 A. |
|---|
| Related PDB | 1try |
|---|
| Related Swiss-prot | P35049 |
|---|
| [45] |
|---|
| PubMed ID | 7503719 |
|---|
| Journal | Biochem Biophys Res Commun |
|---|
| Year | 1995 |
|---|
| Volume | 217 |
|---|
| Pages | 437-44 |
|---|
| Authors | Ascenzi P, Balliano G, Milla P, Ferraccioli R, Sartori P, Djinovic-Carugo K, Bolognesi M |
|---|
| Title | Inhibition of bovine beta-trypsin by the active site titrant N alpha-(N,N-dimethylcarbamoyl)-alpha-azaornithine p-nitrophenyl ester: a kinetic and X-ray crystallographic study. |
|---|
| [46] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 7599119 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1995 |
|---|
| Volume | 34 |
|---|
| Pages | 8264-80 |
|---|
| Authors | Katz BA, Finer-Moore J, Mortezaei R, Rich DH, Stroud RM |
|---|
| Title | Episelection: novel Ki approximately nanomolar inhibitors of serine proteases selected by binding or chemistry on an enzyme surface. |
|---|
| Related PDB | 1btw,1btx,1bty,1btz |
|---|
| [47] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 7548040 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1995 |
|---|
| Volume | 34 |
|---|
| Pages | 12849-53 |
|---|
| Authors | Odagaki Y, Nakai H, Senokuchi K, Kawamura M, Hamanaka N, Nakamura M, Tomoo K, Ishida T |
|---|
| Title | Unique binding of a novel synthetic inhibitor, N-[3-[4-[4-(amidinophenoxy)carbonyl]phenyl]-2-methyl-2-propenoyl]- N-allylglycine methanesulfonate, to bovine trypsin, revealed by the crystal structure of the complex. |
|---|
| Related PDB | 1btp |
|---|
| [48] |
|---|
| PubMed ID | 7703351 |
|---|
| Journal | Biophys Chem |
|---|
| Year | 1995 |
|---|
| Volume | 54 |
|---|
| Pages | 75-81 |
|---|
| Authors | Casale E, Collyer C, Ascenzi P, Balliano G, Milla P, Viola F, Fasano M, Menegatti E, Bolognesi M |
|---|
| Title | Inhibition of bovine beta-trypsin, human alpha-thrombin and porcine pancreatic beta-kallikrein-B by 4',6-diamidino-2-phenylindole, 6-amidinoindole and benzamidine: a comparative thermodynamic and X-ray structural study. |
|---|
| [49] |
|---|
| PubMed ID | 7821429 |
|---|
| Journal | FEBS Lett |
|---|
| Year | 1995 |
|---|
| Volume | 358 |
|---|
| Pages | 53-6 |
|---|
| Authors | Sartori P, Djinovic Carugo K, Ferraccioli R, Balliano G, Milla P, Ascenzi P, Bolognesi M |
|---|
| Title | Active site titration of bovine beta-trypsin by N alpha-(N,N-dimethylcarbamoyl)-alpha-aza-lysine p-nitrophenyl ester: kinetic and crystallographic analysis. |
|---|
| [50] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 7498454 |
|---|
| Journal | FEBS Lett |
|---|
| Year | 1995 |
|---|
| Volume | 375 |
|---|
| Pages | 103-7 |
|---|
| Authors | Stubbs MT, Huber R, Bode W |
|---|
| Title | Crystal structures of factor Xa specific inhibitors in complex with trypsin: structural grounds for inhibition of factor Xa and selectivity against thrombin. |
|---|
| Related PDB | 1mts,1mtu,1mtv,1mtw |
|---|
| [51] |
|---|
| PubMed ID | 8749303 |
|---|
| Journal | J Biochem (Tokyo) |
|---|
| Year | 1995 |
|---|
| Volume | 118 |
|---|
| Pages | 882-94 |
|---|
| Authors | Yamane T, Iwasaki A, Suzuki A, Ashida T, Kawata Y |
|---|
| Title | Crystal structure of Streptomyces erythraeus trypsin at 1.9 A resolution. |
|---|
| [52] |
|---|
| PubMed ID | 7616573 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1995 |
|---|
| Volume | 250 |
|---|
| Pages | 553-70 |
|---|
| Authors | McDowell RS, Kossiakoff AA |
|---|
| Title | A comparison of neutron diffraction and molecular dynamics structures: hydroxyl group and water molecule orientations in trypsin. |
|---|
| [53] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 7795518 |
|---|
| Journal | Protein Sci |
|---|
| Year | 1995 |
|---|
| Volume | 4 |
|---|
| Pages | 337-60 |
|---|
| Authors | Perona JJ, Craik CS |
|---|
| Title | Structural basis of substrate specificity in the serine proteases. |
|---|
| Related PDB | 1amh |
|---|
| [54] |
|---|
| PubMed ID | 9092457 |
|---|
| Journal | Basic Life Sci |
|---|
| Year | 1996 |
|---|
| Volume | 64 |
|---|
| Pages | 273-87 |
|---|
| Authors | McDowell RS, Kossiakoff AA |
|---|
| Title | Hydroxyl and water molecule orientations in trypsin: comparison to molecular dynamic structures. |
|---|
| [55] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 8605148 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1996 |
|---|
| Volume | 35 |
|---|
| Pages | 3147-55 |
|---|
| Authors | Bertrand JA, Oleksyszyn J, Kam CM, Boduszek B, Presnell S, Plaskon RR, Suddath FL, Powers JC, Williams LD |
|---|
| Title | Inhibition of trypsin and thrombin by amino(4-amidinophenyl)methanephosphonate diphenyl ester derivatives: X-ray structures and molecular models. |
|---|
| Related PDB | 1max,1may |
|---|
| [56] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS). |
|---|
| Medline ID | 96214506 |
|---|
| PubMed ID | 8634241 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1996 |
|---|
| Volume | 35 |
|---|
| Pages | 5999-6009 |
|---|
| Authors | Brinen LS, Willett WS, Craik CS, Fletterick RJ |
|---|
| Title | X-ray structures of a designed binding site in trypsin show metal-dependent geometry. |
|---|
| Related PDB | 1slu,1slv,1slw,1slx |
|---|
| Related Swiss-prot | P00763 |
|---|
| [57] |
|---|
| PubMed ID | 8960786 |
|---|
| Journal | Clin Chim Acta |
|---|
| Year | 1996 |
|---|
| Volume | 256 |
|---|
| Pages | 37-51 |
|---|
| Authors | Finotti P |
|---|
| Title | Separation by heparin-affinity chromatography of catalytically active and inactive forms of trypsin which retain the (Na-K)ATPase stimulating property. |
|---|
| [58] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), AND PHOSPHORYLATION. |
|---|
| Medline ID | 96266496 |
|---|
| PubMed ID | 8683601 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1996 |
|---|
| Volume | 259 |
|---|
| Pages | 995-1010 |
|---|
| Authors | Gaboriaud C, Serre L, Guy-Crotte O, Forest E, Fontecilla-Camps JC |
|---|
| Title | Crystal structure of human trypsin 1: unexpected phosphorylation of Tyr151. |
|---|
| Related PDB | 1trn |
|---|
| Related Swiss-prot | P07477 |
|---|
| [59] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 8845765 |
|---|
| Journal | Protein Sci |
|---|
| Year | 1996 |
|---|
| Volume | 5 |
|---|
| Pages | 752-8 |
|---|
| Authors | Kurinov IV, Harrison RW |
|---|
| Title | Two crystal structures of the leupeptin-trypsin complex. |
|---|
| Related PDB | 1jrs,1jrt |
|---|
| [60] |
|---|
| Comments | X-ray crystallography |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 1997 |
|---|
| Volume | 53 |
|---|
| Pages | 311-5 |
|---|
| Authors | Johnson A, Krishnaswamy S, Sundaram PV, Pattabhi V |
|---|
| Title | The First Structure at 1.8 A Resolution of an Active Autolysate Form of Porcine [alpha]-Trysoin. |
|---|
| Related PDB | 1aks |
|---|
| [61] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9341205 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1997 |
|---|
| Volume | 36 |
|---|
| Pages | 13180-6 |
|---|
| Authors | Lee SL, Alexander RS, Smallwood A, Trievel R, Mersinger L, Weber PC, Kettner C |
|---|
| Title | New inhibitors of thrombin and other trypsin-like proteases: hydrogen bonding of an aromatic cyano group with a backbone amide of the P1 binding site replaces binding of a basic side chain. |
|---|
| Related PDB | 1auj |
|---|
| [62] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF COMPLEX WITH LDTI. |
|---|
| Medline ID | 97390427 |
|---|
| PubMed ID | 9242660 |
|---|
| Journal | J Biol Chem |
|---|
| Year | 1997 |
|---|
| Volume | 272 |
|---|
| Pages | 19931-7 |
|---|
| Authors | Stubbs MT, Morenweiser R, Sturzebecher J, Bauer M, Bode W, Huber R, Piechottka GP, Matschiner G, Sommerhoff CP, Fritz H, Auerswald EA |
|---|
| Title | The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition. |
|---|
| Related PDB | 1ldt |
|---|
| Related Swiss-prot | P00761 |
|---|
| [63] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9199408 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1997 |
|---|
| Volume | 269 |
|---|
| Pages | 395-407 |
|---|
| Authors | Burgering MJ, Orbons LP, van der Doelen A, Mulders J, Theunissen HJ, Grootenhuis PD, Bode W, Huber R, Stubbs MT |
|---|
| Title | The second Kunitz domain of human tissue factor pathway inhibitor: cloning, structure determination and interaction with factor Xa. |
|---|
| Related PDB | 1tfx |
|---|
| [64] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9300481 |
|---|
| Journal | Protein Sci |
|---|
| Year | 1997 |
|---|
| Volume | 6 |
|---|
| Pages | 1806-24 |
|---|
| Authors | Scheidig AJ, Hynes TR, Pelletier LA, Wells JA, Kossiakoff AA |
|---|
| Title | Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities. |
|---|
| Related PDB | 1taw |
|---|
| [65] |
|---|
| Comments | X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF COMPLEX WITH LDTI. |
|---|
| Medline ID | 98046095 |
|---|
| PubMed ID | 9384562 |
|---|
| Journal | Structure |
|---|
| Year | 1997 |
|---|
| Volume | 5 |
|---|
| Pages | 1465-74 |
|---|
| Authors | Di Marco S, Priestle JP |
|---|
| Title | Structure of the complex of leech-derived tryptase inhibitor (LDTI) with trypsin and modeling of the LDTI-tryptase system. |
|---|
| Related PDB | 1an1 |
|---|
| Related Swiss-prot | P00761 |
|---|
| [66] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9757092 |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 1998 |
|---|
| Volume | 54 |
|---|
| Pages | 780-98 |
|---|
| Authors | Schroder HK, Willassen NP, Smalas AO |
|---|
| Title | Structure of a non-psychrophilic trypsin from a cold-adapted fish species. |
|---|
| Related PDB | 1a0j |
|---|
| [67] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9724521 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1998 |
|---|
| Volume | 37 |
|---|
| Pages | 12094-103 |
|---|
| Authors | Krishnan R, Zhang E, Hakansson K, Arni RK, Tulinsky A, Lim-Wilby MS, Levy OE, Semple JE, Brunck TK |
|---|
| Title | Highly selective mechanism-based thrombin inhibitors: structures of thrombin and trypsin inhibited with rigid peptidyl aldehydes. |
|---|
| Related PDB | 1yyy,1zzz |
|---|
| [68] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9836602 |
|---|
| Journal | Biochemistry |
|---|
| Year | 1998 |
|---|
| Volume | 37 |
|---|
| Pages | 17068-81 |
|---|
| Authors | Presnell SR, Patil GS, Mura C, Jude KM, Conley JM, Bertrand JA, Kam CM, Powers JC, Williams LD |
|---|
| Title | Oxyanion-mediated inhibition of serine proteases. |
|---|
| Related PDB | 1bju,1bjv |
|---|
| [69] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9920392 |
|---|
| Journal | Biochim Biophys Acta |
|---|
| Year | 1998 |
|---|
| Volume | 1429 |
|---|
| Pages | 142-50 |
|---|
| Authors | Zhu G, Huang Q, Wang Z, Qian M, Jia Y, Tang Y |
|---|
| Title | X-ray studies on two forms of bovine beta-trypsin crystals in neat cyclohexane. |
|---|
| Related PDB | 1tio,2tio |
|---|
| [70] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9760170 |
|---|
| Journal | Eur J Biochem |
|---|
| Year | 1998 |
|---|
| Volume | 256 |
|---|
| Pages | 317-24 |
|---|
| Authors | Helland R, Leiros I, Berglund GI, Willassen NP, Smalas AO |
|---|
| Title | The crystal structure of anionic salmon trypsin in complex with bovine pancreatic trypsin inhibitor. |
|---|
| Related PDB | 1bzx |
|---|
| [71] |
|---|
| Comments | X-ray crystallography |
|---|
| Journal | J Am Chem Soc |
|---|
| Year | 1998 |
|---|
| Volume | 120 |
|---|
| Pages | 595-6 |
|---|
| Authors | Sandler B, Murakami M, Clardy J |
|---|
| Title | Atomic Structure of the Trypsin-Aeruginosin 98-B Complex. |
|---|
| Related PDB | 1aq7 |
|---|
| [72] |
|---|
| PubMed ID | 9876114 |
|---|
| Journal | J Med Chem |
|---|
| Year | 1998 |
|---|
| Volume | 41 |
|---|
| Pages | 5445-56 |
|---|
| Authors | Renatus M, Bode W, Huber R, Sturzebecher J, Stubbs MT |
|---|
| Title | Structural and functional analyses of benzamidine-based inhibitors in complex with trypsin: implications for the inhibition of factor Xa, tPA, and urokinase. |
|---|
| [73] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9466914 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1998 |
|---|
| Volume | 275 |
|---|
| Pages | 347-63 |
|---|
| Authors | Song HK, Suh SW |
|---|
| Title | Kunitz-type soybean trypsin inhibitor revisited: refined structure of its complex with porcine trypsin reveals an insight into the interaction between a homologous inhibitor from Erythrina caffra and tissue-type plasminogen activator. |
|---|
| Related PDB | 1avw,1avx |
|---|
| [74] |
|---|
| PubMed ID | 9495588 |
|---|
| Journal | J Pept Res |
|---|
| Year | 1998 |
|---|
| Volume | 51 |
|---|
| Pages | 29-37 |
|---|
| Authors | Bemquerer MP, Liria CW, Kitagawa K, Miranda MT, Tominaga M |
|---|
| Title | Mixtures of trifluoroethanol or hexafluoroisopropanol and dimethylformamide are not of general applicability for peptide condensations catalyzed by trypsin. |
|---|
| [75] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9468142 |
|---|
| Journal | Nature |
|---|
| Year | 1998 |
|---|
| Volume | 391 |
|---|
| Pages | 608-12 |
|---|
| Authors | Katz BA, Clark JM, Finer-Moore JS, Jenkins TE, Johnson CR, Ross MJ, Luong C, Moore WR, Stroud RM |
|---|
| Title | Design of potent selective zinc-mediated serine protease inhibitors. |
|---|
| Related PDB | 1c1n,1c1o,1c1p,1c1q,1c1r,1c1s,1c1t,1c2d,1c2e,1c2f,1c2g,1c2h,1c2i,1c2j,1c2k,1c2l,1c2m,1xuf,1xug,1xuh,1xui,1xuj,1xuk |
|---|
| [76] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 9707558 |
|---|
| Journal | Proc Natl Acad Sci U S A |
|---|
| Year | 1998 |
|---|
| Volume | 95 |
|---|
| Pages | 9813-8 |
|---|
| Authors | Hopfner KP, Kopetzki E, Kresse GB, Bode W, Huber R, Engh RA |
|---|
| Title | New enzyme lineages by subdomain shuffling. |
|---|
| Related PDB | 1fxy |
|---|
| [77] |
|---|
| PubMed ID | 9749919 |
|---|
| Journal | Protein Eng |
|---|
| Year | 1998 |
|---|
| Volume | 11 |
|---|
| Pages | 669-73 |
|---|
| Authors | Hung SH, Hedstrom L |
|---|
| Title | Converting trypsin to elastase: substitution of the S1 site and adjacent loops reconstitutes esterase specificity but not amidase activity. |
|---|
| [78] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10089404 |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 1999 |
|---|
| Volume | 55 |
|---|
| Pages | 139-48 |
|---|
| Authors | Helland R, Berglund GI, Otlewski J, Apostoluk W, Andersen OA, Willassen NP, Smalas AO |
|---|
| Title | High-resolution structures of three new trypsin-squash-inhibitor complexes: a detailed comparison with other trypsins and their complexes. |
|---|
| Related PDB | 2btc,2sta,2stb |
|---|
| [79] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10531473 |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 1999 |
|---|
| Volume | 55 |
|---|
| Pages | 1785-91 |
|---|
| Authors | Recacha R, Carson M, Costanzo MJ, Maryanoff B, DeLucas LJ, Chattopadhyay D |
|---|
| Title | Structure of the RWJ-51084-bovine pancreatic beta-trypsin complex at 1.8 A. |
|---|
| Related PDB | 1qcp |
|---|
| [80] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10417407 |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 1999 |
|---|
| Volume | 55 |
|---|
| Pages | 1395-404 |
|---|
| Authors | Whitlow M, Arnaiz DO, Buckman BO, Davey DD, Griedel B, Guilford WJ, Koovakkat SK, Liang A, Mohan R, Phillips GB, Seto M, Shaw KJ, Xu W, Zhao Z, Light DR, Morrissey MM |
|---|
| Title | Crystallographic analysis of potent and selective factor Xa inhibitors complexed to bovine trypsin. |
|---|
| Related PDB | 1qa0,1qb1,1qb6,1qb9,1qbn,1qbo |
|---|
| [81] |
|---|
| PubMed ID | 9989934 |
|---|
| Journal | Arch Biochem Biophys |
|---|
| Year | 1999 |
|---|
| Volume | 362 |
|---|
| Pages | 254-64 |
|---|
| Authors | Kaslik G, Westler WM, Graf L, Markley JL |
|---|
| Title | Properties of the His57-Asp102 dyad of rat trypsin D189S in the zymogen, activated enzyme, and alpha1-proteinase inhibitor complexed forms. |
|---|
| [82] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10561533 |
|---|
| Journal | Biochim Biophys Acta |
|---|
| Year | 1999 |
|---|
| Volume | 1435 |
|---|
| Pages | 7-21 |
|---|
| Authors | Johnson A, Gautham N, Pattabhi V |
|---|
| Title | Crystal structure at 1.63 A resolution of the native form of porcine beta-trypsin: revealing an acetate ion binding site and functional water network. |
|---|
| Related PDB | 1qqu |
|---|
| [83] |
|---|
| PubMed ID | 10429182 |
|---|
| Journal | Eur J Biochem |
|---|
| Year | 1999 |
|---|
| Volume | 263 |
|---|
| Pages | 20-6 |
|---|
| Authors | Szabo E, Bocskei Z, Naray-Szabo G, Graf L |
|---|
| Title | The three-dimensional structure of Asp189Ser trypsin provides evidence for an inherent structural plasticity of the protease. |
|---|
| [84] |
|---|
| PubMed ID | 10641732 |
|---|
| Journal | Free Radic Biol Med |
|---|
| Year | 1999 |
|---|
| Volume | 27 |
|---|
| Pages | 1378-85 |
|---|
| Authors | Finotti P, Corvaja C, Pagetta A |
|---|
| Title | Differential mechanisms for structural and functional alterations of trypsin by heparin, evidence for a specific, radical-generating mechanism at low heparin concentrations. |
|---|
| [85] |
|---|
| PubMed ID | 10517520 |
|---|
| Journal | Int J Biol Macromol |
|---|
| Year | 1999 |
|---|
| Volume | 26 |
|---|
| Pages | 135-44 |
|---|
| Authors | Finotti P, Pagetta A, Corvaja C |
|---|
| Title | Role of reducing terminals in unfractionated and low-molecular-mass heparins in causing free radical generation and loss of structure and activity of trypsin. |
|---|
| [86] |
|---|
| PubMed ID | 10373424 |
|---|
| Journal | J Biol Chem |
|---|
| Year | 1999 |
|---|
| Volume | 274 |
|---|
| Pages | 18231-6 |
|---|
| Authors | Lin CY, Anders J, Johnson M, Sang QA, Dickson RB |
|---|
| Title | Molecular cloning of cDNA for matriptase, a matrix-degrading serine protease with trypsin-like activity. |
|---|
| [87] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10447205 |
|---|
| Journal | J Biomol Struct Dyn |
|---|
| Year | 1999 |
|---|
| Volume | 16 |
|---|
| Pages | 1219-24 |
|---|
| Authors | Zhu Y, Huang Q, Chi C |
|---|
| Title | Crystal structure of mung bean inhibitor lysine active fragment complex with bovine beta-trypsin at 1.8A resolution. |
|---|
| Related PDB | 1sbw |
|---|
| [88] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10222201 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1999 |
|---|
| Volume | 287 |
|---|
| Pages | 923-42 |
|---|
| Authors | Helland R, Otlewski J, Sundheim O, Dadlez M, Smalas AO |
|---|
| Title | The crystal structures of the complexes between bovine beta-trypsin and ten P1 variants of BPTI. |
|---|
| Related PDB | 3btd,3bte,3btf,3btg,3bth,3btk,3btm,3btq,3btt,3btw |
|---|
| [89] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10390350 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1999 |
|---|
| Volume | 290 |
|---|
| Pages | 525-33 |
|---|
| Authors | Luckett S, Garcia RS, Barker JJ, Konarev AV, Shewry PR, Clarke AR, Brady RL |
|---|
| Title | High-resolution structure of a potent, cyclic proteinase inhibitor from sunflower seeds. |
|---|
| Related PDB | 1sfi |
|---|
| [90] |
|---|
| PubMed ID | 10356328 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1999 |
|---|
| Volume | 289 |
|---|
| Pages | 547-64 |
|---|
| Authors | Nakasako M |
|---|
| Title | Large-scale networks of hydration water molecules around bovine beta-trypsin revealed by cryogenic X-ray crystal structure analysis. |
|---|
| [91] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10512718 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 1999 |
|---|
| Volume | 293 |
|---|
| Pages | 93-106 |
|---|
| Authors | Rester U, Bode W, Moser M, Parry MA, Huber R, Auerswald E |
|---|
| Title | Structure of the complex of the antistasin-type inhibitor bdellastasin with trypsin and modelling of the bdellastasin-microplasmin system. |
|---|
| Related PDB | 1c9p,1c9t |
|---|
| [92] |
|---|
| PubMed ID | 10591040 |
|---|
| Journal | J Nat Toxins |
|---|
| Year | 1999 |
|---|
| Volume | 8 |
|---|
| Pages | 363-84 |
|---|
| Authors | Azim MK, Grossmann JG, Zaidi ZH |
|---|
| Title | Predicted three-dimensional structural models of venom serine protease inhibitors and their interactions with trypsin and chymotrypsin. |
|---|
| [93] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10524768 |
|---|
| Journal | J Protein Chem |
|---|
| Year | 1999 |
|---|
| Volume | 18 |
|---|
| Pages | 505-9 |
|---|
| Authors | Zhu Y, Huang Q, Qian M, Jia Y, Tang Y |
|---|
| Title | Crystal structure of the complex formed between bovine beta-trypsin and MCTI-A, a trypsin inhibitor of squash family, at 1.8-A resolution. |
|---|
| Related PDB | 1f2s,1mcu |
|---|
| [94] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10210204 |
|---|
| Journal | Protein Sci |
|---|
| Year | 1999 |
|---|
| Volume | 8 |
|---|
| Pages | 253-8 |
|---|
| Authors | Pasternak A, Ringe D, Hedstrom L |
|---|
| Title | Comparison of anionic and cationic trypsinogens: the anionic activation domain is more flexible in solution and differs in its mode of BPTI binding in the crystal structure. |
|---|
| Related PDB | 3tgi,3tgj |
|---|
| [95] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10651279 |
|---|
| Journal | Proteins |
|---|
| Year | 1999 |
|---|
| Volume | 37 |
|---|
| Pages | 641-53 |
|---|
| Authors | Ota N, Stroupe C, Ferreira-da-Silva JM, Shah SA, Mares-Guia M, Brunger AT |
|---|
| Title | Non-Boltzmann thermodynamic integration (NBTI) for macromolecular systems: relative free energy of binding of trypsin to benzamidine and benzylamine. |
|---|
| Related PDB | 1ce5,2bza |
|---|
| [96] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10771427 |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 2000 |
|---|
| Volume | 56 |
|---|
| Pages | 581-8 |
|---|
| Authors | Rester U, Moser M, Huber R, Bode W |
|---|
| Title | L-Isoaspartate 115 of porcine beta-trypsin promotes crystallization of its complex with bdellastasin. |
|---|
| Related PDB | 1eja |
|---|
| [97] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10779411 |
|---|
| Journal | Chem Biol |
|---|
| Year | 2000 |
|---|
| Volume | 7 |
|---|
| Pages | 299-312 |
|---|
| Authors | Katz BA, Mackman R, Luong C, Radika K, Martelli A, Sprengeler PA, Wang J, Chan H, Wong L |
|---|
| Title | Structural basis for selectivity of a small molecule, S1-binding, submicromolar inhibitor of urokinase-type plasminogen activator. |
|---|
| Related PDB | 1c5p,1c5q,1c5r,1c5s,1c5t,1c5u,1c5v |
|---|
| [98] |
|---|
| PubMed ID | 11126764 |
|---|
| Journal | Comp Biochem Physiol B Biochem Mol Biol |
|---|
| Year | 2000 |
|---|
| Volume | 127 |
|---|
| Pages | 337-46 |
|---|
| Authors | Sekizaki H, Itoh K, Murakami M, Toyota E, Tanizawa K |
|---|
| Title | Anionic trypsin from chum salmon: activity with p-amidinophenyl ester and comparison with bovine and Streptomyces griseus trypsins. |
|---|
| [99] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10966741 |
|---|
| Journal | J Med Chem |
|---|
| Year | 2000 |
|---|
| Volume | 43 |
|---|
| Pages | 3226-32 |
|---|
| Authors | Maignan S, Guilloteau JP, Pouzieux S, Choi-Sledeski YM, Becker MR, Klein SI, Ewing WR, Pauls HW, Spada AP, Mikol V |
|---|
| Title | Crystal structures of human factor Xa complexed with potent inhibitors. |
|---|
| Related PDB | 1f0t,1f0u |
|---|
| [100] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10843853 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2000 |
|---|
| Volume | 299 |
|---|
| Pages | 993-1003 |
|---|
| Authors | Gillmor SA, Takeuchi T, Yang SQ, Craik CS, Fletterick RJ |
|---|
| Title | Compromise and accommodation in ecotin, a dimeric macromolecular inhibitor of serine proteases. |
|---|
| Related PDB | 1ezs,1ezu |
|---|
| [101] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10926503 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2000 |
|---|
| Volume | 301 |
|---|
| Pages | 205-17 |
|---|
| Authors | Grzesiak A, Helland R, Smalas AO, Krowarsch D, Dadlez M, Otlewski J |
|---|
| Title | Substitutions at the P(1) position in BPTI strongly affect the association energy with serine proteinases. |
|---|
| Related PDB | 1ejm |
|---|
| [102] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 10772864 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2000 |
|---|
| Volume | 298 |
|---|
| Pages | 477-91 |
|---|
| Authors | Koepke J, Ermler U, Warkentin E, Wenzl G, Flecker P |
|---|
| Title | Crystal structure of cancer chemopreventive Bowman-Birk inhibitor in ternary complex with bovine trypsin at 2.3 A resolution. Structural basis of Janus-faced serine protease inhibitor specificity. |
|---|
| Related PDB | 1d6r |
|---|
| [103] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11057674 |
|---|
| Journal | Nature |
|---|
| Year | 2000 |
|---|
| Volume | 407 |
|---|
| Pages | 923-6 |
|---|
| Authors | Huntington JA, Read RJ, Carrell RW |
|---|
| Title | Structure of a serpin-protease complex shows inhibition by deformation. |
|---|
| Related PDB | 1ezx |
|---|
| [104] |
|---|
| PubMed ID | 10944388 |
|---|
| Journal | Proteins |
|---|
| Year | 2000 |
|---|
| Volume | 41 |
|---|
| Pages | 8-16 |
|---|
| Authors | Pletnev VZ, Zamolodchikova TS, Pangborn WA, Duax WL |
|---|
| Title | Crystal structure of bovine duodenase, a serine protease, with dual trypsin and chymotrypsin-like specificities. |
|---|
| [105] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11679713 |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 2001 |
|---|
| Volume | 57 |
|---|
| Pages | 1506-12 |
|---|
| Authors | Deepthi S, Johnson A, Pattabhi V |
|---|
| Title | Structures of porcine beta-trypsin-detergent complexes: the stabilization of proteins through hydrophilic binding of polydocanol. |
|---|
| Related PDB | 1fmg,1fn6,1fni |
|---|
| [106] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11264577 |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 2001 |
|---|
| Volume | 57 |
|---|
| Pages | 488-97 |
|---|
| Authors | Leiros HK, McSweeney SM, Smalas AO |
|---|
| Title | Atomic resolution structures of trypsin provide insight into structural radiation damage. |
|---|
| Related PDB | 1hj8,1hj9 |
|---|
| [107] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11134922 |
|---|
| Journal | Acta Crystallogr D Biol Crystallogr |
|---|
| Year | 2001 |
|---|
| Volume | 57 |
|---|
| Pages | 8-19 |
|---|
| Authors | Rypniewski WR, Ostergaard PR, Norregaard-Madsen M, Dauter M, Wilson KS |
|---|
| Title | Fusarium oxysporum trypsin at atomic resolution at 100 and 283 K: a study of ligand binding. |
|---|
| Related PDB | 1fn8,1fy4,1fy5,1gdn,1gdq,1gdu |
|---|
| [108] |
|---|
| PubMed ID | 11371189 |
|---|
| Journal | Biochemistry |
|---|
| Year | 2001 |
|---|
| Volume | 40 |
|---|
| Pages | 6275-83 |
|---|
| Authors | Peterson FC, Gordon NC, Gettins PG |
|---|
| Title | High-level bacterial expression and 15N-alanine-labeling of bovine trypsin. Application to the study of trypsin-inhibitor complexes and trypsinogen activation by NMR spectroscopy. |
|---|
| [109] |
|---|
| PubMed ID | 11731085 |
|---|
| Journal | Biochim Biophys Acta |
|---|
| Year | 2001 |
|---|
| Volume | 1568 |
|---|
| Pages | 53-9 |
|---|
| Authors | Park H, Chi YM |
|---|
| Title | The enhancement of electrostriction caused by lowering the solvent dielectric constant leads to the decrease of activation energy in trypsin catalysis. |
|---|
| [110] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11731301 |
|---|
| Journal | Chem Biol |
|---|
| Year | 2001 |
|---|
| Volume | 8 |
|---|
| Pages | 1107-21 |
|---|
| Authors | Katz BA, Sprengeler PA, Luong C, Verner E, Elrod K, Kirtley M, Janc J, Spencer JR, Breitenbucher JG, Hui H, McGee D, Allen D, Martelli A, Mackman RL |
|---|
| Title | Engineering inhibitors highly selective for the S1 sites of Ser190 trypsin-like serine protease drug targets. |
|---|
| Related PDB | 1gj6 |
|---|
| [111] |
|---|
| PubMed ID | 11508835 |
|---|
| Journal | Eur Biophys J |
|---|
| Year | 2001 |
|---|
| Volume | 30 |
|---|
| Pages | 163-70 |
|---|
| Authors | Caracciolo G, Amiconi G, Bencivenni L, Boumis G, Caminiti R, Finocchiaro E, Maras B, Paolinelli C, Congiu Castellano A |
|---|
| Title | Conformational study of proteins by SAXS and EDXD: the case of trypsin and trypsinogen. |
|---|
| [112] |
|---|
| PubMed ID | 11226886 |
|---|
| Journal | J Biochem (Tokyo) |
|---|
| Year | 2001 |
|---|
| Volume | 129 |
|---|
| Pages | 455-60 |
|---|
| Authors | Tomoo K, Satoh K, Tsuda Y, Wanaka K, Okamoto S, Hijikata-Okunomiya A, Okada Y, Ishida T |
|---|
| Title | Binding diversity of a noncovalent-type low-molecular-weight serine protease inhibitor and function of a catalytic water molecule: X-ray crystal structure of PKSI-527--inhibited trypsin. |
|---|
| [113] |
|---|
| PubMed ID | 11312265 |
|---|
| Journal | J Biol Chem |
|---|
| Year | 2001 |
|---|
| Volume | 276 |
|---|
| Pages | 24574-80 |
|---|
| Authors | Szilagyi L, Kenesi E, Katona G, Kaslik G, Juhasz G, Graf L |
|---|
| Title | Comparative in vitro studies on native and recombinant human cationic trypsins. Cathepsin B is a possible pathological activator of trypsinogen in pancreatitis. |
|---|
| [114] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11676542 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2001 |
|---|
| Volume | 313 |
|---|
| Pages | 593-614 |
|---|
| Authors | Dullweber F, Stubbs MT, Musil D, Sturzebecher J, Klebe G |
|---|
| Title | Factorising ligand affinity: a combined thermodynamic and crystallographic study of trypsin and thrombin inhibition. |
|---|
| Related PDB | 1k1i,1k1j,1k1l,1k1m,1k1n,1k1o,1k1p |
|---|
| [115] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11292354 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2001 |
|---|
| Volume | 307 |
|---|
| Pages | 1451-86 |
|---|
| Authors | Katz BA, Elrod K, Luong C, Rice MJ, Mackman RL, Sprengeler PA, Spencer J, Hataye J, Janc J, Link J, Litvak J, Rai R, Rice K, Sideris S, Verner E, Young W |
|---|
| Title | A novel serine protease inhibition motif involving a multi-centered short hydrogen bonding network at the active site. |
|---|
| Related PDB | 1ghz,1gi0,1gi1,1gi2,1gi3,1gi4,1gi5,1gi6 |
|---|
| [116] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11152605 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2001 |
|---|
| Volume | 305 |
|---|
| Pages | 471-9 |
|---|
| Authors | Toyota E, Ng KK, Sekizaki H, Itoh K, Tanizawa K, James MN |
|---|
| Title | X-ray crystallographic analyses of complexes between bovine beta-trypsin and Schiff base copper(II) or iron(III) chelates. |
|---|
| Related PDB | 1g3b,1g3c,1g3d,1g3e |
|---|
| [117] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11685246 |
|---|
| Journal | Nat Struct Biol |
|---|
| Year | 2001 |
|---|
| Volume | 8 |
|---|
| Pages | 979-83 |
|---|
| Authors | Ye S, Cech AL, Belmares R, Bergstrom RC, Tong Y, Corey DR, Kanost MR, Goldsmith EJ |
|---|
| Title | The structure of a Michaelis serpin-protease complex. |
|---|
| Related PDB | 1i99,1k9o |
|---|
| [118] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11420435 |
|---|
| Journal | Protein Sci |
|---|
| Year | 2001 |
|---|
| Volume | 10 |
|---|
| Pages | 1331-42 |
|---|
| Authors | Pasternak A, White A, Jeffery CJ, Medina N, Cahoon M, Ringe D, Hedstrom L |
|---|
| Title | The energetic cost of induced fit catalysis: Crystal structures of trypsinogen mutants with enhanced activity and inhibitor affinity. |
|---|
| Related PDB | 1fy8,1f5r,1f7z,3tgk |
|---|
| [119] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11342132 |
|---|
| Journal | Structure (Camb) |
|---|
| Year | 2001 |
|---|
| Volume | 9 |
|---|
| Pages | 29-37 |
|---|
| Authors | Nar H, Bauer M, Schmid A, Stassen JM, Wienen W, Priepke HW, Kauffmann IK, Ries UJ, Hauel NH |
|---|
| Title | Structural basis for inhibition promiscuity of dual specific thrombin and factor Xa blood coagulation inhibitors. |
|---|
| Related PDB | 1g36,1oyq |
|---|
| [120] |
|---|
| PubMed ID | 11779198 |
|---|
| Journal | Biochem Biophys Res Commun |
|---|
| Year | 2002 |
|---|
| Volume | 290 |
|---|
| Pages | 494-7 |
|---|
| Authors | Marana SR, Lopes AR, Juliano L, Juliano MA, Ferreira C, Terra WR |
|---|
| Title | Subsites of trypsin active site favor catalysis or substrate binding. |
|---|
| [121] |
|---|
| PubMed ID | 11921406 |
|---|
| Journal | Chembiochem |
|---|
| Year | 2002 |
|---|
| Volume | 3 |
|---|
| Pages | 246-9 |
|---|
| Authors | Stubbs MT, Reyda S, Dullweber F, Moller M, Klebe G, Dorsch D, Mederski WW, Wurziger H |
|---|
| Title | pH-dependent binding modes observed in trypsin crystals: lessons for structure-based drug design. |
|---|
| Related PDB | 1ql7,1ql8,1ql9 |
|---|
| [122] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 12061878 |
|---|
| Journal | J Med Chem |
|---|
| Year | 2002 |
|---|
| Volume | 45 |
|---|
| Pages | 2749-69 |
|---|
| Authors | Matter H, Defossa E, Heinelt U, Blohm PM, Schneider D, Muller A, Herok S, Schreuder H, Liesum A, Brachvogel V, Lonze P, Walser A, Al-Obeidi F, Wildgoose P |
|---|
| Title | Design and quantitative structure-activity relationship of 3-amidinobenzyl-1H-indole-2-carboxamides as potent, nonchiral, and selective inhibitors of blood coagulation factor Xa. |
|---|
| Related PDB | 1lqe |
|---|
| [123] |
|---|
| PubMed ID | 11827488 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2002 |
|---|
| Volume | 315 |
|---|
| Pages | 1209-18 |
|---|
| Authors | Katona G, Berglund GI, Hajdu J, Graf L, Szilagyi L |
|---|
| Title | Crystal structure reveals basis for the inhibitor resistance of human brain trypsin. |
|---|
| [124] |
|---|
| PubMed ID | 12445776 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2002 |
|---|
| Volume | 324 |
|---|
| Pages | 391-7 |
|---|
| Authors | Toyota E, Ng KK, Kuninaga S, Sekizaki H, Itoh K, Tanizawa K, James MN |
|---|
| Title | Crystal structure and nucleotide sequence of an anionic trypsin from chum salmon (Oncorhynchus keta) in comparison with Atlantic salmon (Salmo salar) and bovine trypsin. |
|---|
| [125] |
|---|
| PubMed ID | 12705877 |
|---|
| Journal | Biochem Biophys Res Commun |
|---|
| Year | 2003 |
|---|
| Volume | 304 |
|---|
| Pages | 18-21 |
|---|
| Authors | Kotorman M, Laczko I, Szabo A, Simon LM |
|---|
| Title | Effects of Ca2+ on catalytic activity and conformation of trypsin and alpha-chymotrypsin in aqueous ethanol. |
|---|
| [126] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 12885239 |
|---|
| Journal | Biochemistry |
|---|
| Year | 2003 |
|---|
| Volume | 42 |
|---|
| Pages | 9060-6 |
|---|
| Authors | Page MJ, Wong SL, Hewitt J, Strynadka NC, MacGillivray RT |
|---|
| Title | Engineering the primary substrate specificity of Streptomyces griseus trypsin. |
|---|
| Related PDB | 1os8,1oss |
|---|
| [127] |
|---|
| PubMed ID | 12837799 |
|---|
| Journal | J Bacteriol |
|---|
| Year | 2003 |
|---|
| Volume | 185 |
|---|
| Pages | 4233-42 |
|---|
| Authors | Sudom A, Walters R, Pastushok L, Goldie D, Prasad L, Delbaere LT, Goldie H |
|---|
| Title | Mechanisms of activation of phosphoenolpyruvate carboxykinase from Escherichia coli by Ca2+ and of desensitization by trypsin. |
|---|
| [128] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 12860985 |
|---|
| Journal | J Biol Chem |
|---|
| Year | 2003 |
|---|
| Volume | 278 |
|---|
| Pages | 37881-7 |
|---|
| Authors | Dementiev A, Simonovic M, Volz K, Gettins PG |
|---|
| Title | Canonical inhibitor-like interactions explain reactivity of alpha1-proteinase inhibitor Pittsburgh and antithrombin with proteinases. |
|---|
| Related PDB | 1oph |
|---|
| [129] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 12937176 |
|---|
| Journal | J Biol Chem |
|---|
| Year | 2003 |
|---|
| Volume | 278 |
|---|
| Pages | 43357-62 |
|---|
| Authors | Schmidt A, Jelsch C, Ostergaard P, Rypniewski W, Lamzin VS |
|---|
| Title | Trypsin revisited: crystallography AT (SUB) atomic resolution and quantum chemistry revealing details of catalysis. |
|---|
| Related PDB | 1ppz,1pq5,1pq7,1pq8,1pqa |
|---|
| [130] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 12930148 |
|---|
| Journal | J Med Chem |
|---|
| Year | 2003 |
|---|
| Volume | 46 |
|---|
| Pages | 3865-76 |
|---|
| Authors | Costanzo MJ, Yabut SC, Almond HR Jr, Andrade-Gordon P, Corcoran TW, De Garavilla L, Kauffman JA, Abraham WM, Recacha R, Chattopadhyay D, Maryanoff BE |
|---|
| Title | Potent, small-molecule inhibitors of human mast cell tryptase. Antiasthmatic action of a dipeptide-based transition-state analogue containing a benzothiazole ketone. |
|---|
| Related PDB | 1nc6 |
|---|
| [131] |
|---|
| PubMed ID | 14568540 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2003 |
|---|
| Volume | 333 |
|---|
| Pages | 845-61 |
|---|
| Authors | Helland R, Czapinska H, Leiros I, Olufsen M, Otlewski J, Smalas AO |
|---|
| Title | Structural consequences of accommodation of four non-cognate amino acid residues in the S1 pocket of bovine trypsin and chymotrypsin. |
|---|
| [132] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 12742021 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2003 |
|---|
| Volume | 329 |
|---|
| Pages | 93-120 |
|---|
| Authors | Katz BA, Elrod K, Verner E, Mackman RL, Luong C, Shrader WD, Sendzik M, Spencer JR, Sprengeler PA, Kolesnikov A, Tai VW, Hui HC, Breitenbucher JG, Allen D, Janc JW |
|---|
| Title | Elaborate manifold of short hydrogen bond arrays mediating binding of active site-directed serine protease inhibitors. |
|---|
| Related PDB | 1o2h,1o2i,1o2j,1o2k,1o2l,1o2m,1o2n,1o2o,1o2p,1o2q,1o2r,1o2s,1o2t,1o2u,1o2v,1o2w,1o2x,1o2y,1o2z,1o30,1o31,1o32,1o33,1o34,1o35,1o36,1o37,1o38,1o39,1o3a,1o3b,1o3c,1o3d,1o3e,1o3f,1o3g,1o3h,1o3i,1o3j,1o3k,1o3l,1o3m,1o3n,1o3o |
|---|
| [133] |
|---|
| PubMed ID | 12527302 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2003 |
|---|
| Volume | 325 |
|---|
| Pages | 963-77 |
|---|
| Authors | Reyda S, Sohn C, Klebe G, Rall K, Ullmann D, Jakubke HD, Stubbs MT |
|---|
| Title | Reconstructing the binding site of factor Xa in trypsin reveals ligand-induced structural plasticity. |
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| Related PDB | 1j14,1j15,1j16,1j17 |
|---|
| [134] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 12575941 |
|---|
| Journal | Structure (Camb) |
|---|
| Year | 2003 |
|---|
| Volume | 11 |
|---|
| Pages | 217-24 |
|---|
| Authors | Ravelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S |
|---|
| Title | Specific radiation damage can be used to solve macromolecular crystal structures. |
|---|
| Related PDB | 1n6x,1n6y |
|---|
| [135] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 14672690 |
|---|
| Journal | Biochem Biophys Res Commun |
|---|
| Year | 2004 |
|---|
| Volume | 313 |
|---|
| Pages | 8-16 |
|---|
| Authors | Ibrahim BS, Pattabhi V |
|---|
| Title | Crystal structure of trypsin-turkey egg white inhibitor complex. |
|---|
| Related PDB | 1r0t |
|---|
| [136] |
|---|
| PubMed ID | 14729347 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2004 |
|---|
| Volume | 335 |
|---|
| Pages | 1325-41 |
|---|
| Authors | Rauh D, Klebe G, Stubbs MT |
|---|
| Title | Understanding protein-ligand interactions: the price of protein flexibility. |
|---|
| [137] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 15044735 |
|---|
| Journal | Protein Sci |
|---|
| Year | 2004 |
|---|
| Volume | 13 |
|---|
| Pages | 1056-70 |
|---|
| Authors | Leiros HK, Brandsdal BO, Andersen OA, Os V, Leiros I, Helland R, Otlewski J, Willassen NP, Smalas AO |
|---|
| Title | Trypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements. |
|---|
| Related PDB | 1utj,1utk,1utl,1utm,1utn,1uto,1utp,1utq |
|---|
| comments | The chains of the enzymes deposited in the following PDB structures are split into two or three fragments.
PDB;1aksA, Swiss-prot;TRYP_PIG;P00761, residues 9-133 (PDB;16-145)
PDB;1aksB, Swiss-prot;TRYP_PIG;P00761, residues 134-231 (PDB;146-245)
PDB;1eptA, Swiss-prot;TRYP_PIG;P00761, residues 9-51 (PDB;16-60)
PDB;1eptB, Swiss-prot;TRYP_PIG;P00761, residues 52-133 (PDB;61-145)
PDB;1eptC, Swiss-prot;TRYP_PIG;P00761, residues 132-231 (PDB;146-245)
This enzyme, trypsin, is a calssical serine protease, whose catalytic mechanism has been elucidated by various studies. This enzyme has got a catalytic triad, composed of Ser195/His57/Asp102.
According to the literature [10] and [53], the catalytic reaction proceeds as follows:
(1) His57 acts as a general base, by abstracting a proton from the hydroxyl group of Ser195. Here, Asp102 stabilizes the charged His57, and orients the conformation of His57 correctly (see [10]).
(2) Ser195, whose nucleophilicity was enhanced by His57, makes a nucleophilic attack on the carbonyl carbon atom of the scissile bond, peptide bond, leading to the formation of a tetrahedral acyl-intermediate.
(3) The oxyanion of the intermediate is stabilized by the mainchain amide nitrogens of Gly193 and Ser195.
(4) His57 acts as a general acid, by protonating to the newly formed amine group. This leads to the dissociation of the first product.
(5) A water molecule makes a nucleophilic attack on the acyl group, resulting in the deacylation.
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| created | updated |
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| 2004-09-17 | 2009-02-26 |
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