EzCatDB: D00197

DB codeD00197
RLCP classification1.13.30000.10
CATH domainDomain 12.40.10.10Catalytic domain
Domain 22.40.10.10Catalytic domain
E.C.3.4.21.4
CSA1a0j

CATH domainRelated DB codes (homologues)
2.40.10.10M00139,D00214,M00167,D00426,M00133,D00428,D00429,D00430,D00431,D00432,D00433,D00434,D00435,M00227,M00209,D00194,D00211,D00212,D00216,M00212,D00224,D00497,M00217,M00216,D00528,D00848,D00850,D00851,D00852,D00855,M00152,M00155,M00157,M00181,M00315,M00316,M00317,M00348,M00349,T00074,T00410,T00411

Enzyme Name
Swiss-protKEGG

P00775P35049P35031P35033P00762P00763P00761P00760P07477
Protein nameTrypsinTrypsinTrypsin-1Trypsin-3Anionic trypsin-1Anionic trypsin-2TrypsinCationic trypsinTrypsin-1trypsin
alpha-trypsin
beta-trypsin
cocoonase
parenzyme
parenzymol
tryptar
trypure
pseudotrypsin
tryptase
tripcellim
sperm receptor hydrolase
SynonymsEC 3.4.21.4
SGT
EC 3.4.21.4
EC 3.4.21.4
Trypsin I
EC 3.4.21.4
Trypsin III
EC 3.4.21.4
Anionic trypsin I
Pretrypsinogen I
Serine protease 1
EC 3.4.21.4
Anionic trypsin II
Pretrypsinogen II
Serine protease 2
EC 3.4.21.4
EC 3.4.21.4
Beta-trypsin
EC 3.4.21.4
Trypsin I
Cationic trypsinogen
Serine protease 1
Beta-trypsin
ContainsNoneNoneNoneNoneNoneNoneNoneAlpha-trypsin chain 1
Alpha-trypsin chain 2
Alpha-trypsin chain 1
Alpha-trypsin chain 2


Swiss-prot:Accession NumberP00775P35049P35031P35033P00762P00763P00761P00760P07477
Entry nameTRYP_STRGRTRYP_FUSOXTRY1_SALSATRY3_SALSATRY1_RATTRY2_RATTRYP_PIGTRY1_BOVINTRY1_HUMAN
ActivityPreferential cleavage: Arg-|-Xaa, Lys-|-Xaa.Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subunit








Subcellular location
Secreted.Secreted, extracellular space.Secreted, extracellular space.Secreted, extracellular space.Secreted, extracellular space.Secreted, extracellular space.Secreted, extracellular space.Secreted, extracellular space.
Cofactor

Binds 1 calcium ion per subunit.Binds 1 calcium ion per subunit (By similarity).Binds 1 calcium ion per subunit.Binds 1 calcium ion per subunit.Binds 1 calcium ion per subunit.Binds 1 calcium ion per subunit.Binds 1 calcium ion per subunit.


SubstratesProductsintermediates
KEGG-idC00012C00001C00012C03253
CompoundPeptideH2OPeptideAmino acid(Arg-, Lys-)
Typepeptide/proteinH2Opeptide/proteinamino acids
1sgtA01Unbound
UnboundUnboundUnbound
1fn8A01Unbound
Bound:GLY-ALA-ARG(chain B)UnboundUnbound
1fy4A01Unbound
UnboundUnboundIntermediate-bound:GLY-ALA-ARG(chain B)
1fy5A01Unbound
Bound:GLY-ALA-LYS(chain B)UnboundUnbound
1gdnA01Unbound
Bound:GLY-ALA-LYS(chain B)UnboundUnbound
1gdqA01Unbound
UnboundUnboundIntermediate-bound:ARG(chain B)
1gduA01Unbound
Bound:GLY-ALA-ARG(chain B)UnboundUnbound
1tryA01Unbound
UnboundUnboundIntermediate-analogue:ISP
1bitA01Unbound
UnboundUnboundUnbound
1bzxE01Bound:LYS 515-ALA 516(chain I)
UnboundUnboundUnbound
1tbsA01Unbound
UnboundUnboundUnbound
2staE01Bound:ARG 505-ILE 506(chain I)
UnboundUnboundUnbound
2stbE01Bound:LYS 505-ILE 506(chain I)
UnboundUnboundUnbound
2tbsA01Unbound
UnboundUnboundUnbound
1a0jA01Unbound
UnboundUnboundUnbound
1a0jB01Unbound
UnboundUnboundUnbound
1a0jC01Unbound
UnboundUnboundUnbound
1a0jD01Unbound
UnboundUnboundUnbound
1braA01Unbound
UnboundUnboundUnbound
1brbE01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
1brcE01Bound:ARG 15-ALA 16(chain I)
UnboundUnboundUnbound
1trmA01Unbound
UnboundUnboundUnbound
1trmB01Unbound
UnboundUnboundUnbound
2trmA01Unbound
UnboundUnboundUnbound
1amhA01Unbound
UnboundUnboundUnbound
1amhB01Unbound
UnboundUnboundUnbound
1anbA01Unbound
UnboundUnboundUnbound
1ancA01Unbound
UnboundUnboundUnbound
1andA01Unbound
UnboundUnboundUnbound
1aneA01Unbound
UnboundUnboundUnbound
1dpoA01Unbound
UnboundUnboundUnbound
1ezsC01Bound:ARG 284-MET 285(chain B)
UnboundUnboundUnbound
1ezsD01Bound:ARG 84-MET 85(chain A)
UnboundUnboundUnbound
1ezuC01Analogue:MET 284-MET 285(chain B)
UnboundUnboundUnbound
1ezuD01Analogue:MET 84-MET 85(chain A)
UnboundUnboundUnbound
1fy8E01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
1i99E01Bound:LYS 353-SER 354(chain I)
UnboundUnboundUnbound
1ql9A01Unbound
UnboundUnboundUnbound
1sluB01Analogue:MET 84-MET 85(chain A)
UnboundUnboundUnbound
1slvB01Analogue:MET 84-MET 85(chain A)
UnboundUnboundUnbound
1slwB01Analogue:MET 84-MET 85(chain A)
UnboundUnboundUnbound
1slxB01Analogue:MET 84-MET 85(chain A)
UnboundUnboundUnbound
3tgiE01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
3tgjE01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
1aksBUnbound
UnboundUnboundUnbound
1an1E01Bound:LYS 8-ILE 9(chain I)
UnboundUnboundUnbound
1avwA01Bound:ARG 563-ILE 564(chain B)
UnboundUnboundUnbound
1avxA01Bound:ARG 563-ILE 564(chain B)
UnboundUnboundUnbound
1ejaA01Bound:LYS 34-VAL 35(chain B)
UnboundUnboundUnbound
1eptCUnbound
UnboundUnboundUnbound
1fmgA01Unbound
UnboundUnboundUnbound
1fn6A01Unbound
UnboundUnboundUnbound
1fniA01Unbound
UnboundUnboundUnbound
1ldtT01Bound:LYS 8-ILE 9(chain L)
UnboundUnboundUnbound
1mctA01Bound:ARG 5-ILE 6(chain I)
UnboundUnboundUnbound
1qquA01Unbound
UnboundUnboundUnbound
1tfxA01Bound:ARG 15-GLY 16(chain C)
UnboundUnboundUnbound
1tfxB01Bound:ARG 15-GLY 16(chain D)
UnboundUnboundUnbound
1aq7A01Unbound
Analogue:AEBUnboundUnbound
1aujA01Unbound
UnboundUnboundIntermediate-analogue:PPB
1az8A01Unbound
UnboundUnboundUnbound
1bjuA01Unbound
UnboundUnboundUnbound
1bjvA01Unbound
UnboundUnboundUnbound
1btpA01Unbound
UnboundUnboundUnbound
1btwA01Unbound
UnboundUnboundIntermediate-analogue:BOC-ALA-VAL-BLY(chain H)
1btxA01Unbound
UnboundUnboundIntermediate-analogue:BOC-ALA-VAL-BLY-ETH(chain H)
1btyA01Unbound
UnboundUnboundUnbound
1btzA01Unbound
UnboundUnboundIntermediate-analogue:BOC-ALA-VAL-BLY-CH3(chain H)
1c1nA01Unbound
UnboundUnboundUnbound
1c1oA01Unbound
UnboundUnboundUnbound
1c1pA01Unbound
UnboundUnboundUnbound
1c1qA01Unbound
UnboundUnboundUnbound
1c1rA01Unbound
UnboundUnboundUnbound
1c1sA01Unbound
UnboundUnboundUnbound
1c1tA01Unbound
UnboundUnboundUnbound
1c2dA01Unbound
UnboundUnboundUnbound
1c2eA01Unbound
UnboundUnboundUnbound
1c2fA01Unbound
UnboundUnboundUnbound
1c2gA01Unbound
UnboundUnboundUnbound
1c2hA01Unbound
UnboundUnboundUnbound
1c2iA01Unbound
UnboundUnboundUnbound
1c2jA01Unbound
UnboundUnboundUnbound
1c2kA01Unbound
UnboundUnboundUnbound
1c2lA01Unbound
UnboundUnboundUnbound
1c2mA01Unbound
UnboundUnboundUnbound
1c5pA01Unbound
UnboundUnboundUnbound
1c5qA01Unbound
UnboundUnboundUnbound
1c5rA01Unbound
UnboundUnboundUnbound
1c5sA01Unbound
UnboundUnboundUnbound
1c5tA01Unbound
UnboundUnboundUnbound
1c5uA01Unbound
UnboundUnboundUnbound
1c5vA01Unbound
UnboundUnboundUnbound
1c9pA01Bound:LYS 34-VAL 35(chain B)
UnboundUnboundUnbound
1ce5A01Unbound
UnboundUnboundUnbound
1d6rA01Bound:LYS 16-SER 17(chain I)
UnboundUnboundUnbound
1ejmA01Bound:ARG 515-LEU 516(chain B)
UnboundUnboundUnbound
1ejmC01Bound:ARG 515-LEU 516(chain D)
UnboundUnboundUnbound
1ejmE01Bound:ARG 515-LEU 516(chain F)
UnboundUnboundUnbound
1f0tA01Unbound
UnboundUnboundUnbound
1f0uA01Unbound
UnboundUnboundUnbound
1f2sE01Bound:ARG 305-ILE 306(chain I)
UnboundUnboundUnbound
1g3bA01Unbound
UnboundUnboundUnbound
1g3cA01Unbound
UnboundUnboundUnbound
1g3dA01Unbound
UnboundUnboundUnbound
1g3eA01Unbound
UnboundUnboundUnbound
1g9iE01Bound:LYS 311-SER 312(chain I)
UnboundUnboundUnbound
1gbtA01Unbound
UnboundUnboundIntermediate-analogue:GBS
1jrsA01Unbound
UnboundUnboundIntermediate-bound:ACE-LEU-LEU-ARG(chain B)
1jrtA01Unbound
UnboundUnboundIntermediate-bound:ACE-LEU-LEU-ARG(chain B)
1maxA01Unbound
UnboundUnboundIntermediate-analogue:ZAP
1mayA01Unbound
UnboundUnboundIntermediate-analogue:ZAP
1mcuE01Bound:ARG 305-ILE 306(chain I)
UnboundUnboundUnbound
1mtsA01Unbound
UnboundUnboundUnbound
1mtuA01Unbound
UnboundUnboundUnbound
1mtvA01Unbound
UnboundUnboundUnbound
1mtwA01Unbound
UnboundUnboundUnbound
1ntpA01Unbound
UnboundUnboundIntermediate-analogue:MIP
1ppcE01Unbound
Analogue:NAS-GLY-APH-PIP(chain I)UnboundUnbound
1ppeE01Bound:ARG 5-ILE 6(chain I)
UnboundUnboundUnbound
1pphE01Unbound
Analogue:TOS-APM-PIP(chain I)UnboundUnbound
1qa0A01Unbound
UnboundUnboundUnbound
1qb1A01Unbound
UnboundUnboundUnbound
1qb6A01Unbound
UnboundUnboundUnbound
1qb9A01Unbound
UnboundUnboundUnbound
1qbnA01Unbound
UnboundUnboundUnbound
1qboA01Unbound
UnboundUnboundUnbound
1ql7A01Unbound
UnboundUnboundUnbound
1ql8A01Unbound
UnboundUnboundUnbound
1sbwA01Bound:LYS 20-SER 21(chain I)
UnboundUnboundUnbound
1sfiA01Bound:LYS 5-SER 6(chain I)
UnboundUnboundUnbound
1smfE01Bound:LYS 11-SER 12(chain I)
UnboundUnboundUnbound
1tabE01Bound:LYS 26-SER 27(chain I)
UnboundUnboundUnbound
1tawA01Bound:ARG 15-ALA 16(chain B)
UnboundUnboundUnbound
1tgbA01Unbound
UnboundUnboundUnbound
1tgcA01Unbound
UnboundUnboundUnbound
1tgnA01Unbound
UnboundUnboundUnbound
1tgsZ01Bound:LYS 18-ILE 19(chain I)
UnboundUnboundUnbound
1tgtA01Unbound
UnboundUnboundUnbound
1tioA01Unbound
UnboundUnboundUnbound
1tldA01Unbound
UnboundUnboundUnbound
1tngA01Unbound
UnboundUnboundUnbound
1tnhA01Unbound
UnboundUnboundUnbound
1tniA01Unbound
UnboundUnboundUnbound
1tnjA01Unbound
UnboundUnboundUnbound
1tnkA01Unbound
UnboundUnboundUnbound
1tnlA01Unbound
UnboundUnboundUnbound
1tpaE01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
1tpoA01Unbound
UnboundUnboundUnbound
1tppA01Unbound
UnboundUnboundIntermediate-analogue:APA
1tpsA01Analogue:A9A
UnboundUnboundUnbound
1tynA01Unbound
UnboundUnboundIntermediate-analogue:CTA
1xufA01Unbound
UnboundUnboundUnbound
1xugA01Unbound
UnboundUnboundUnbound
1xuhA01Unbound
UnboundUnboundUnbound
1xuiA01Unbound
UnboundUnboundUnbound
1xujA01Unbound
UnboundUnboundUnbound
1xukA01Unbound
UnboundUnboundUnbound
1yyy101Unbound
UnboundUnboundIntermediate-analogue:BNS-NVA-GLY-3GA
2btcE01Bound:LYS 505-ILE 506(chain I)
UnboundUnboundUnbound
2bzaA01Unbound
UnboundUnboundUnbound
2ptcE01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
2ptnA01Unbound
UnboundUnboundUnbound
2tgaA01Unbound
UnboundUnboundUnbound
2tgdA01Unbound
UnboundUnboundIntermediate-analogue:DFP
2tgpZ01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
2tgtA01Unbound
UnboundUnboundUnbound
2tioA01Unbound
UnboundUnboundUnbound
2tpiZ01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
3btdE01Analogue:ASP 515-ALA 516(chain I)
UnboundUnboundUnbound
3bteE01Analogue:GLU 515-ALA 516(chain I)
UnboundUnboundUnbound
3btfE01Analogue:PHE 515-ALA 516(chain I)
UnboundUnboundUnbound
3btgE01Analogue:GLY 515-ALA 516(chain I)
UnboundUnboundUnbound
3bthE01Analogue:HIS 515-ALA 516(chain I)
UnboundUnboundUnbound
3btkE01Bound:LYS 515-ALA 516(chain I)
UnboundUnboundUnbound
3btmE01Analogue:MET 515-ALA 516(chain I)
UnboundUnboundUnbound
3btqE01Analogue:GLN 515-ALA 516(chain I)
UnboundUnboundUnbound
3bttE01Analogue:THR 515-ALA 516(chain I)
UnboundUnboundUnbound
3btwE01Analogue:TRP 515-ALA 516(chain I)
UnboundUnboundUnbound
3ptbA01Unbound
UnboundUnboundUnbound
3ptnA01Unbound
UnboundUnboundUnbound
3tpiZ01Bound:LYS 15-ALA 16(chain I)
UnboundUnboundUnbound
4ptpA01Unbound
UnboundUnboundIntermediate-analogue:DIP
4tpiZ01Bound:ARG 15-ALA 16(chain I)
UnboundUnboundUnbound
5ptpA01Unbound
UnboundUnboundIntermediate-analogue:MIS
1ezxC01Unbound
Bound:SER 359(chain B)UnboundIntermediate-bound:MET 358(chain A)
1trnA01Unbound
UnboundUnboundIntermediate-analogue:ISP
1trnB01Unbound
UnboundUnboundIntermediate-analogue:ISP
1sgtA02Unbound
UnboundUnboundUnbound
1fn8A02Unbound
UnboundUnboundUnbound
1fy4A02Unbound
UnboundUnboundUnbound
1fy5A02Unbound
UnboundUnboundUnbound
1gdnA02Unbound
UnboundUnboundUnbound
1gdqA02Unbound
UnboundUnboundUnbound
1gduA02Unbound
UnboundUnboundUnbound
1tryA02Unbound
UnboundUnboundUnbound
1bitA02Unbound
UnboundUnboundUnbound
1bzxE02Unbound
UnboundUnboundUnbound
1tbsA02Unbound
UnboundUnboundUnbound
2staE02Unbound
UnboundUnboundUnbound
2stbE02Unbound
UnboundUnboundUnbound
2tbsA02Unbound
UnboundUnboundUnbound
1a0jA02Unbound
UnboundUnboundUnbound
1a0jB02Unbound
UnboundUnboundUnbound
1a0jC02Unbound
UnboundUnboundUnbound
1a0jD02Unbound
UnboundUnboundUnbound
1braA02Unbound
UnboundUnboundUnbound
1brbE02Unbound
UnboundUnboundUnbound
1brcE02Unbound
UnboundUnboundUnbound
1trmA02Unbound
UnboundUnboundUnbound
1trmB02Unbound
UnboundUnboundUnbound
2trmA02Unbound
UnboundUnboundUnbound
1amhA02Unbound
UnboundUnboundUnbound
1amhB02Unbound
UnboundUnboundUnbound
1anbA02Unbound
UnboundUnboundUnbound
1ancA02Unbound
UnboundUnboundUnbound
1andA02Unbound
UnboundUnboundUnbound
1aneA02Unbound
UnboundUnboundUnbound
1dpoA02Unbound
UnboundUnboundUnbound
1ezsC02Unbound
UnboundUnboundUnbound
1ezsD02Unbound
UnboundUnboundUnbound
1ezuC02Unbound
UnboundUnboundUnbound
1ezuD02Unbound
UnboundUnboundUnbound
1fy8E02Unbound
UnboundUnboundUnbound
1i99E02Unbound
UnboundUnboundUnbound
1ql9A02Unbound
UnboundUnboundUnbound
1sluB02Unbound
UnboundUnboundUnbound
1slvB02Unbound
UnboundUnboundUnbound
1slwB02Unbound
UnboundUnboundUnbound
1slxB02Unbound
UnboundUnboundUnbound
3tgiE02Unbound
UnboundUnboundUnbound
3tgjE02Unbound
UnboundUnboundUnbound
1aksAUnbound
UnboundUnboundUnbound
1an1E02Unbound
UnboundUnboundUnbound
1avwA02Unbound
UnboundUnboundUnbound
1avxA02Unbound
UnboundUnboundUnbound
1ejaA02Unbound
UnboundUnboundUnbound
1eptAUnbound
UnboundUnboundUnbound
1eptBUnbound
UnboundUnboundUnbound
1fmgA02Unbound
UnboundUnboundUnbound
1fn6A02Unbound
UnboundUnboundUnbound
1fniA02Unbound
UnboundUnboundUnbound
1ldtT02Unbound
UnboundUnboundUnbound
1mctA02Unbound
UnboundUnboundUnbound
1qquA02Unbound
UnboundUnboundUnbound
1tfxA02Unbound
UnboundUnboundUnbound
1tfxB02Unbound
UnboundUnboundUnbound
1aq7A02Unbound
UnboundUnboundUnbound
1aujA02Unbound
UnboundUnboundUnbound
1az8A02Unbound
UnboundUnboundUnbound
1bjuA02Unbound
UnboundUnboundUnbound
1bjvA02Unbound
UnboundUnboundUnbound
1btpA02Unbound
UnboundUnboundUnbound
1btwA02Unbound
UnboundUnboundUnbound
1btxA02Unbound
UnboundUnboundUnbound
1btyA02Unbound
UnboundUnboundUnbound
1btzA02Unbound
UnboundUnboundUnbound
1c1nA02Unbound
UnboundUnboundUnbound
1c1oA02Unbound
UnboundUnboundUnbound
1c1pA02Unbound
UnboundUnboundUnbound
1c1qA02Unbound
UnboundUnboundUnbound
1c1rA02Unbound
UnboundUnboundUnbound
1c1sA02Unbound
UnboundUnboundUnbound
1c1tA02Unbound
UnboundUnboundUnbound
1c2dA02Unbound
UnboundUnboundUnbound
1c2eA02Unbound
UnboundUnboundUnbound
1c2fA02Unbound
UnboundUnboundUnbound
1c2gA02Unbound
UnboundUnboundUnbound
1c2hA02Unbound
UnboundUnboundUnbound
1c2iA02Unbound
UnboundUnboundUnbound
1c2jA02Unbound
UnboundUnboundUnbound
1c2kA02Unbound
UnboundUnboundUnbound
1c2lA02Unbound
UnboundUnboundUnbound
1c2mA02Unbound
UnboundUnboundUnbound
1c5pA02Unbound
UnboundUnboundUnbound
1c5qA02Unbound
UnboundUnboundUnbound
1c5rA02Unbound
UnboundUnboundUnbound
1c5sA02Unbound
UnboundUnboundUnbound
1c5tA02Unbound
UnboundUnboundUnbound
1c5uA02Unbound
UnboundUnboundUnbound
1c5vA02Unbound
UnboundUnboundUnbound
1c9pA02Unbound
UnboundUnboundUnbound
1ce5A02Unbound
UnboundUnboundUnbound
1d6rA02Unbound
UnboundUnboundUnbound
1ejmA02Unbound
UnboundUnboundUnbound
1ejmC02Unbound
UnboundUnboundUnbound
1ejmE02Unbound
UnboundUnboundUnbound
1f0tA02Unbound
UnboundUnboundUnbound
1f0uA02Unbound
UnboundUnboundUnbound
1f2sE02Unbound
UnboundUnboundUnbound
1g3bA02Unbound
UnboundUnboundUnbound
1g3cA02Unbound
UnboundUnboundUnbound
1g3dA02Unbound
UnboundUnboundUnbound
1g3eA02Unbound
UnboundUnboundUnbound
1g9iE02Unbound
UnboundUnboundUnbound
1gbtA02Unbound
UnboundUnboundUnbound
1jrsA02Unbound
UnboundUnboundUnbound
1jrtA02Unbound
UnboundUnboundUnbound
1maxA02Unbound
UnboundUnboundUnbound
1mayA02Unbound
UnboundUnboundUnbound
1mcuE02Unbound
UnboundUnboundUnbound
1mtsA02Unbound
UnboundUnboundUnbound
1mtuA02Unbound
UnboundUnboundUnbound
1mtvA02Unbound
UnboundUnboundUnbound
1mtwA02Unbound
UnboundUnboundUnbound
1ntpA02Unbound
UnboundUnboundUnbound
1ppcE02Unbound
UnboundUnboundUnbound
1ppeE02Unbound
UnboundUnboundUnbound
1pphE02Unbound
UnboundUnboundUnbound
1qa0A02Unbound
UnboundUnboundUnbound
1qb1A02Unbound
UnboundUnboundUnbound
1qb6A02Unbound
UnboundUnboundUnbound
1qb9A02Unbound
UnboundUnboundUnbound
1qbnA02Unbound
UnboundUnboundUnbound
1qboA02Unbound
UnboundUnboundUnbound
1ql7A02Unbound
UnboundUnboundUnbound
1ql8A02Unbound
UnboundUnboundUnbound
1sbwA02Unbound
UnboundUnboundUnbound
1sfiA02Unbound
UnboundUnboundUnbound
1smfE02Unbound
UnboundUnboundUnbound
1tabE02Unbound
UnboundUnboundUnbound
1tawA02Unbound
UnboundUnboundUnbound
1tgbA02Unbound
UnboundUnboundUnbound
1tgcA02Unbound
UnboundUnboundUnbound
1tgnA02Unbound
UnboundUnboundUnbound
1tgsZ02Unbound
UnboundUnboundUnbound
1tgtA02Unbound
UnboundUnboundUnbound
1tioA02Unbound
UnboundUnboundUnbound
1tldA02Unbound
UnboundUnboundUnbound
1tngA02Unbound
UnboundUnboundUnbound
1tnhA02Unbound
UnboundUnboundUnbound
1tniA02Unbound
UnboundUnboundUnbound
1tnjA02Unbound
UnboundUnboundUnbound
1tnkA02Unbound
UnboundUnboundUnbound
1tnlA02Unbound
UnboundUnboundUnbound
1tpaE02Unbound
UnboundUnboundUnbound
1tpoA02Unbound
UnboundUnboundUnbound
1tppA02Unbound
UnboundUnboundUnbound
1tpsA02Unbound
UnboundUnboundUnbound
1tynA02Unbound
UnboundUnboundUnbound
1xufA02Unbound
UnboundUnboundUnbound
1xugA02Unbound
UnboundUnboundUnbound
1xuhA02Unbound
UnboundUnboundUnbound
1xuiA02Unbound
UnboundUnboundUnbound
1xujA02Unbound
UnboundUnboundUnbound
1xukA02Unbound
UnboundUnboundUnbound
1yyy102Unbound
UnboundUnboundUnbound
2btcE02Unbound
UnboundUnboundUnbound
2bzaA02Unbound
UnboundUnboundUnbound
2ptcE02Unbound
UnboundUnboundUnbound
2ptnA02Unbound
UnboundUnboundUnbound
2tgaA02Unbound
UnboundUnboundUnbound
2tgdA02Unbound
UnboundUnboundUnbound
2tgpZ02Unbound
UnboundUnboundUnbound
2tgtA02Unbound
UnboundUnboundUnbound
2tioA02Unbound
UnboundUnboundUnbound
2tpiZ02Unbound
UnboundUnboundUnbound
3btdE02Unbound
UnboundUnboundUnbound
3bteE02Unbound
UnboundUnboundUnbound
3btfE02Unbound
UnboundUnboundUnbound
3btgE02Unbound
UnboundUnboundUnbound
3bthE02Unbound
UnboundUnboundUnbound
3btkE02Unbound
UnboundUnboundUnbound
3btmE02Unbound
UnboundUnboundUnbound
3btqE02Unbound
UnboundUnboundUnbound
3bttE02Unbound
UnboundUnboundUnbound
3btwE02Unbound
UnboundUnboundUnbound
3ptbA02Unbound
UnboundUnboundUnbound
3ptnA02Unbound
UnboundUnboundUnbound
3tpiZ02Unbound
UnboundUnboundUnbound
4ptpA02Unbound
UnboundUnboundUnbound
4tpiZ02Unbound
UnboundUnboundUnbound
5ptpA02Unbound
UnboundUnboundUnbound
1ezxC02Unbound
UnboundUnboundUnbound
1trnA02Unbound
UnboundUnboundUnbound
1trnB02Unbound
UnboundUnboundUnbound

Active-site residues
pdbCatalytic residuesModified residuesMain-chain involved in catalysiscomment
1sgtA01SER 195

GLY 193;SER 195

1fn8A01SER 195

GLY 193;SER 195

1fy4A01SER 195

GLY 193;SER 195

1fy5A01SER 195

GLY 193;SER 195

1gdnA01SER 195

GLY 193;SER 195

1gdqA01SER 195

GLY 193;SER 195

1gduA01SER 195

GLY 193;SER 195

1tryA01SER 195

GLY 193;SER 195

1bitA01SER 195

GLY 193;SER 195

1bzxE01SER 195

GLY 193;SER 195

1tbsA01SER 195

GLY 193;SER 195

2staE01SER 195

GLY 193;SER 195

2stbE01SER 195

GLY 193;SER 195

2tbsA01SER 195

GLY 193;SER 195

1a0jA01SER 195

GLY 193;SER 195

1a0jB01SER 195

GLY 193;SER 195

1a0jC01SER 195

GLY 193;SER 195

1a0jD01SER 195

GLY 193;SER 195

1braA01SER 195

GLY 193;SER 195
mutant D189G, G226D
1brbE01SER 195

GLY 193;SER 195
mutant D189G, G226D
1brcE01SER 195

GLY 193;SER 195
mutant D189G, G226D
1trmA01SER 195

GLY 193;SER 195

1trmB01SER 195

GLY 193;SER 195

2trmA01SER 195

GLY 193;SER 195

1amhA01SER 195

GLY 193;SER 195
mutant D189S
1amhB01SER 195

GLY 193;SER 195
mutant D189S
1anbA01SER 195

GLY 193;SER 195
mutant S214E
1ancA01SER 195

GLY 193;SER 195
mutant S214K
1andA01SER 195

GLY 193;SER 195

1aneA01SER 195

GLY 193;SER 195

1dpoA01       

GLY 193;       
mutant S195C
1ezsC01SER 595

GLY 593;SER 595

1ezsD01SER 895

GLY 893;SER 895

1ezuC01SER 595

GLY 593;SER 595

1ezuD01SER 895

GLY 893;SER 895

1fy8E01SER 195

GLY 193;SER 195

1i99E01       

GLY 193;       
mutant S195A
1ql9A01SER 195

GLY 193;SER 195

1sluB01SER 195

GLY 193;SER 195
mutant N143H, E151H
1slvB01SER 195

GLY 193;SER 195
mutant N143H, E151H
1slwB01SER 195

GLY 193;SER 195
mutant V121I, N143H, E151H
1slxB01SER 195

GLY 193;SER 195
mutant N143H, E151H
3tgiE01SER 195

GLY 193;SER 195

3tgjE01       

GLY 193;       
mutant S195A
1aksBSER 195

GLY 193;SER 195

1an1E01SER 195

GLY 193;SER 195

1avwA01SER 195

GLY 193;SER 195

1avxA01SER 195

GLY 193;SER 195

1ejaA01SER 195

GLY 193;SER 195

1eptCSER 195

GLY 193;SER 195

1fmgA01SER 195

GLY 193;SER 195

1fn6A01SER 195

GLY 193;SER 195

1fniA01SER 195

GLY 193;SER 195

1ldtT01SER 195

GLY 193;SER 195

1mctA01SER 195

GLY 193;SER 195

1qquA01SER 195

GLY 193;SER 195

1tfxA01SER 195

GLY 193;SER 195

1tfxB01SER 195

GLY 193;SER 195

1aq7A01SER 195

GLY 193;SER 195

1aujA01SER 195

GLY 193;SER 195

1az8A01SER 195

GLY 193;SER 195

1bjuA01SER 195

GLY 193;SER 195

1bjvA01SER 195

GLY 193;SER 195

1btpA01SER 195

GLY 193;SER 195

1btwA01SER 195

GLY 193;SER 195

1btxA01SER 195

GLY 193;SER 195

1btyA01SER 195

GLY 193;SER 195

1btzA01SER 195

GLY 193;SER 195

1c1nA01SER 195

GLY 193;SER 195

1c1oA01SER 195

GLY 193;SER 195

1c1pA01SER 195

GLY 193;SER 195

1c1qA01SER 195

GLY 193;SER 195

1c1rA01SER 195

GLY 193;SER 195

1c1sA01SER 195

GLY 193;SER 195

1c1tA01SER 195

GLY 193;SER 195

1c2dA01SER 195

GLY 193;SER 195

1c2eA01SER 195

GLY 193;SER 195

1c2fA01SER 195

GLY 193;SER 195

1c2gA01SER 195

GLY 193;SER 195

1c2hA01SER 195

GLY 193;SER 195

1c2iA01SER 195

GLY 193;SER 195

1c2jA01SER 195

GLY 193;SER 195

1c2kA01SER 195

GLY 193;SER 195

1c2lA01SER 195

GLY 193;SER 195

1c2mA01SER 195

GLY 193;SER 195

1c5pA01SER 195

GLY 193;SER 195

1c5qA01SER 195

GLY 193;SER 195

1c5rA01SER 195

GLY 193;SER 195

1c5sA01SER 195

GLY 193;SER 195

1c5tA01SER 195

GLY 193;SER 195

1c5uA01SER 195

GLY 193;SER 195

1c5vA01SER 195

GLY 193;SER 195

1c9pA01SER 195

GLY 193;SER 195

1ce5A01SER 195

GLY 193;SER 195

1d6rA01SER 195

GLY 193;SER 195

1ejmA01SER 195

GLY 193;SER 195
mutant A16L
1ejmC01SER 195

GLY 193;SER 195
mutant A16L
1ejmE01SER 195

GLY 193;SER 195
mutant A16L
1f0tA01SER 195

GLY 193;SER 195

1f0uA01SER 195

GLY 193;SER 195

1f2sE01SER 195

GLY 193;SER 195

1g3bA01SER 195

GLY 193;SER 195

1g3cA01SER 195

GLY 193;SER 195

1g3dA01SER 195

GLY 193;SER 195

1g3eA01SER 195

GLY 193;SER 195

1g9iE01SER 195

GLY 193;SER 195

1gbtA01SER 195

GLY 193;SER 195

1jrsA01SER 195

GLY 193;SER 195

1jrtA01SER 195

GLY 193;SER 195

1maxA01SER 195

GLY 193;SER 195

1mayA01SER 195

GLY 193;SER 195

1mcuE01SER 195

GLY 193;SER 195

1mtsA01SER 195

GLY 193;SER 195

1mtuA01SER 195

GLY 193;SER 195

1mtvA01SER 195

GLY 193;SER 195

1mtwA01SER 195

GLY 193;SER 195

1ntpA01SER 195

GLY 193;SER 195

1ppcE01SER 195

GLY 193;SER 195

1ppeE01SER 195

GLY 193;SER 195

1pphE01SER 195

GLY 193;SER 195

1qa0A01SER 183

GLY 181;SER 183

1qb1A01SER 183

GLY 181;SER 183

1qb6A01SER 183

GLY 181;SER 183

1qb9A01SER 183

GLY 181;SER 183

1qbnA01SER 183

GLY 181;SER 183

1qboA01SER 183

GLY 181;SER 183

1ql7A01SER 195

GLY 193;SER 195

1ql8A01SER 195

GLY 193;SER 195

1sbwA01SER 195

GLY 193;SER 195

1sfiA01SER 195

GLY 193;SER 195

1smfE01SER 195

GLY 193;SER 195

1tabE01SER 195

GLY 193;SER 195

1tawA01SER 195

GLY 193;SER 195

1tgbA01SER 195

GLY 193;SER 195

1tgcA01SER 195

GLY 193;SER 195

1tgnA01SER 195

GLY 193;SER 195

1tgsZ01SER 195

GLY 193;SER 195

1tgtA01SER 195

GLY 193;SER 195

1tioA01SER 195

GLY 193;SER 195

1tldA01SER 195

GLY 193;SER 195

1tngA01SER 195

GLY 193;SER 195

1tnhA01SER 195

GLY 193;SER 195

1tniA01SER 195

GLY 193;SER 195

1tnjA01SER 195

GLY 193;SER 195

1tnkA01SER 195

GLY 193;SER 195

1tnlA01SER 195

GLY 193;SER 195

1tpaE01SER 195

GLY 193;SER 195

1tpoA01SER 195

GLY 193;SER 195

1tppA01SER 195

GLY 193;SER 195

1tpsA01SER 195

GLY 193;SER 195

1tynA01SER 195

GLY 193;SER 195

1xufA01SER 195

GLY 193;SER 195

1xugA01SER 195

GLY 193;SER 195

1xuhA01SER 195

GLY 193;SER 195

1xuiA01SER 195

GLY 193;SER 195

1xujA01SER 195

GLY 193;SER 195

1xukA01SER 195

GLY 193;SER 195

1yyy101SER 195

GLY 193;SER 195

2btcE01SER 195

GLY 193;SER 195

2bzaA01SER 195

GLY 193;SER 195

2ptcE01SER 195

GLY 193;SER 195

2ptnA01SER 195

GLY 193;SER 195

2tgaA01SER 195

GLY 193;SER 195

2tgdA01SER 195

GLY 193;SER 195

2tgpZ01SER 195

GLY 193;SER 195

2tgtA01SER 195

GLY 193;SER 195

2tioA01SER 195

GLY 193;SER 195

2tpiZ01SER 195

GLY 193;SER 195

3btdE01SER 195

GLY 193;SER 195

3bteE01SER 195

GLY 193;SER 195

3btfE01SER 195

GLY 193;SER 195

3btgE01SER 195

GLY 193;SER 195

3bthE01SER 195

GLY 193;SER 195

3btkE01SER 195

GLY 193;SER 195

3btmE01SER 195

GLY 193;SER 195

3btqE01SER 195

GLY 193;SER 195

3bttE01SER 195

GLY 193;SER 195

3btwE01SER 195

GLY 193;SER 195

3ptbA01SER 195

GLY 193;SER 195

3ptnA01SER 195

GLY 193;SER 195

3tpiZ01SER 195

GLY 193;SER 195

4ptpA01SER 195

GLY 193;SER 195

4tpiZ01SER 195

GLY 193;SER 195

5ptpA01       
MIS 195(monoisopropyl-phosphorylated)
GLY 193;MIS 195

1ezxC01SER 195

GLY 193;SER 195

1trnA01SER 195

GLY 193;SER 195

1trnB01SER 195

GLY 193;SER 195

1sgtA02HIS  57;ASP 102



1fn8A02HIS  57;ASP 102



1fy4A02HIS  57;ASP 102



1fy5A02HIS  57;ASP 102



1gdnA02HIS  57;ASP 102



1gdqA02HIS  57;ASP 102



1gduA02HIS  57;ASP 102



1tryA02HIS  57;ASP 102



1bitA02HIS  57;ASP 102



1bzxE02HIS  57;ASP 102



1tbsA02HIS  57;ASP 102



2staE02HIS  57;ASP 102



2stbE02HIS  57;ASP 102



2tbsA02HIS  57;ASP 102



1a0jA02HIS  57;ASP 102



1a0jB02HIS  57;ASP 102



1a0jC02HIS  57;ASP 102



1a0jD02HIS  57;ASP 102



1braA02HIS  57;ASP 102



1brbE02HIS  57;ASP 102



1brcE02HIS  57;ASP 102



1trmA02HIS  57;       


mutant D102N
1trmB02HIS  57;       


mutant D102N
2trmA02HIS  57;       


mutant D102N
1amhA02HIS  57;ASP 102



1amhB02HIS  57;ASP 102



1anbA02HIS  57;ASP 102



1ancA02HIS  57;ASP 102



1andA02HIS  57;ASP 102


mutant R96H
1aneA02HIS  57;ASP 102



1dpoA02HIS  57;ASP 102



1ezsC02HIS 457;       


mutant D502N
1ezsD02HIS 757;       


mutant D802N
1ezuC02HIS 457;       


mutant D502N
1ezuD02HIS 757;       


mutant D802N
1fy8E02HIS  57;ASP 102



1i99E02HIS  57;ASP 102



1ql9A02HIS  57;ASP 102



1sluB02HIS  57;ASP 102



1slvB02HIS  57;ASP 102



1slwB02HIS  57;ASP 102



1slxB02HIS  57;ASP 102



3tgiE02HIS  57;ASP 102



3tgjE02HIS  57;ASP 102



1aksAHIS  57;ASP 102



1an1E02HIS  57;ASP 102



1avwA02HIS  57;ASP 102



1avxA02HIS  57;ASP 102



1ejaA02HIS  57;ASP 102



1eptAHIS  57;       



1eptB       ;ASP 102



1fmgA02HIS  57;ASP 102



1fn6A02HIS  57;ASP 102



1fniA02HIS  57;ASP 102



1ldtT02HIS  57;ASP 102



1mctA02HIS  57;ASP 102



1qquA02HIS  57;ASP 102



1tfxA02HIS  57;ASP 102



1tfxB02HIS  57;ASP 102



1aq7A02HIS  57;ASP 102



1aujA02HIS  57;ASP 102



1az8A02HIS  57;ASP 102



1bjuA02HIS  57;ASP 102



1bjvA02HIS  57;ASP 102



1btpA02HIS  57;ASP 102



1btwA02HIS  57;ASP 102



1btxA02HIS  57;ASP 102



1btyA02HIS  57;ASP 102



1btzA02HIS  57;ASP 102



1c1nA02HIS  57;ASP 102



1c1oA02HIS  57;ASP 102



1c1pA02HIS  57;ASP 102



1c1qA02HIS  57;ASP 102



1c1rA02HIS  57;ASP 102



1c1sA02HIS  57;ASP 102



1c1tA02HIS  57;ASP 102



1c2dA02HIS  57;ASP 102



1c2eA02HIS  57;ASP 102



1c2fA02HIS  57;ASP 102



1c2gA02HIS  57;ASP 102



1c2hA02HIS  57;ASP 102



1c2iA02HIS  57;ASP 102



1c2jA02HIS  57;ASP 102



1c2kA02HIS  57;ASP 102



1c2lA02HIS  57;ASP 102



1c2mA02HIS  57;ASP 102



1c5pA02HIS  57;ASP 102



1c5qA02HIS  57;ASP 102



1c5rA02HIS  57;ASP 102



1c5sA02HIS  57;ASP 102



1c5tA02HIS  57;ASP 102



1c5uA02HIS  57;ASP 102



1c5vA02HIS  57;ASP 102



1c9pA02HIS  57;ASP 102



1ce5A02HIS  57;ASP 102



1d6rA02HIS  57;ASP 102



1ejmA02HIS  57;ASP 102



1ejmC02HIS  57;ASP 102



1ejmE02HIS  57;ASP 102



1f0tA02HIS  57;ASP 102



1f0uA02HIS  57;ASP 102



1f2sE02HIS  57;ASP 102



1g3bA02HIS  57;ASP 102



1g3cA02HIS  57;ASP 102



1g3dA02HIS  57;ASP 102



1g3eA02HIS  57;ASP 102



1g9iE02HIS  57;ASP 102



1gbtA02HIS  57;ASP 102



1jrsA02HIS  57;ASP 102



1jrtA02HIS  57;ASP 102



1maxA02HIS  57;ASP 102



1mayA02HIS  57;ASP 102



1mcuE02HIS  57;ASP 102



1mtsA02HIS  57;ASP 102



1mtuA02HIS  57;ASP 102



1mtvA02HIS  57;ASP 102



1mtwA02HIS  57;ASP 102



1ntpA02HIS  57;ASP 102



1ppcE02HIS  57;ASP 102



1ppeE02HIS  57;ASP 102



1pphE02HIS  57;ASP 102



1qa0A02HIS  46;ASP  90



1qb1A02HIS  46;ASP  90



1qb6A02HIS  46;ASP  90



1qb9A02HIS  46;ASP  90



1qbnA02HIS  46;ASP  90



1qboA02HIS  46;ASP  90



1ql7A02HIS  57;ASP 102



1ql8A02HIS  57;ASP 102



1sbwA02HIS  57;ASP 102



1sfiA02HIS  57;ASP 102



1smfE02HIS  57;ASP 102



1tabE02HIS  57;ASP 102



1tawA02HIS  57;ASP 102



1tgbA02HIS  57;ASP 102



1tgcA02HIS  57;ASP 102



1tgnA02HIS  57;ASP 102



1tgsZ02HIS  57;ASP 102



1tgtA02HIS  57;ASP 102



1tioA02HIS  57;ASP 102



1tldA02HIS  57;ASP 102



1tngA02HIS  57;ASP 102



1tnhA02HIS  57;ASP 102



1tniA02HIS  57;ASP 102



1tnjA02HIS  57;ASP 102



1tnkA02HIS  57;ASP 102



1tnlA02HIS  57;ASP 102



1tpaE02HIS  57;ASP 102



1tpoA02HIS  57;ASP 102



1tppA02HIS  57;ASP 102



1tpsA02HIS  57;ASP 102



1tynA02HIS  57;ASP 102



1xufA02HIS  57;ASP 102



1xugA02HIS  57;ASP 102



1xuhA02HIS  57;ASP 102



1xuiA02HIS  57;ASP 102



1xujA02HIS  57;ASP 102



1xukA02HIS  57;ASP 102



1yyy102HIS  57;ASP 102



2btcE02HIS  57;ASP 102



2bzaA02HIS  57;ASP 102



2ptcE02HIS  57;ASP 102



2ptnA02HIS  57;ASP 102



2tgaA02HIS  57;ASP 102



2tgdA02HIS  57;ASP 102



2tgpZ02HIS  57;ASP 102



2tgtA02HIS  57;ASP 102



2tioA02HIS  57;ASP 102



2tpiZ02HIS  57;ASP 102



3btdE02HIS  57;ASP 102



3bteE02HIS  57;ASP 102



3btfE02HIS  57;ASP 102



3btgE02HIS  57;ASP 102



3bthE02HIS  57;ASP 102



3btkE02HIS  57;ASP 102



3btmE02HIS  57;ASP 102



3btqE02HIS  57;ASP 102



3bttE02HIS  57;ASP 102



3btwE02HIS  57;ASP 102



3ptbA02HIS  57;ASP 102



3ptnA02HIS  57;ASP 102



3tpiZ02HIS  57;ASP 102



4ptpA02HIS  57;ASP 102



4tpiZ02HIS  57;ASP 102



5ptpA02HIS  57;ASP 102



1ezxC02HIS  57;ASP 102



1trnA02HIS  57;ASP 102



1trnB02HIS  57;ASP 102




References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[10]p.905
[47]Fig.9
[53]Fig. 2, p.338-3403
[68]p.17079
[73]p.355-356
[78]p.146
[129]Fig.2, p.433624

references
[1]
CommentsX-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF CALCIUM-BINDING SITE.
Medline ID76072097
PubMed ID512
JournalJ Mol Biol
Year1975
Volume98
Pages693-717
AuthorsBode W, Schwager P
TitleThe refined crystal structure of bovine beta-trypsin at 1.8 A resolution. II. Crystallographic refinement, calcium binding site, benzamidine binding site and active site at pH 7.0.
Related Swiss-protP00760
[2]
CommentsX-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
Medline ID77112431
PubMed ID556951
JournalBiochemistry
Year1977
Volume16
Pages654-64
AuthorsKossiakoff AA, Chambers JL, Kay LM, Stroud RM
TitleStructure of bovine trypsinogen at 1.9 A resolution.
Related PDB1tgn
Related Swiss-protP00760
[3]
CommentsX-ray crystallography
PubMed ID864704
JournalJ Mol Biol
Year1977
Volume111
Pages415-38
AuthorsFehlhammer H, Bode W, Huber R
TitleCrystal structure of bovine trypsinogen at 1-8 A resolution. II. Crystallographic refinement, refined crystal structure and comparison with bovine trypsin.
Related PDB1tgb
[4]
CommentsX-ray crystallography
JournalActa Crystallogr B
Year1982
Volume38
Pages1462-72
AuthorsWalter J, Steigemann W, Singh TP, Bartunik H, Bode W, Huber R
TitleOn the disordered activation domain in trypsinogen: chemical labelling and low-temperature crystallography.
Related PDB1tgc,1tgt,2ptn,2tga,2tgt,2tpi,3ptn
[5]
CommentsX-ray crystallography
PubMed ID7169635
JournalJ Mol Biol
Year1982
Volume162
Pages839-68
AuthorsBolognesi M, Gatti G, Menagatti E, Guarneri M, Marquart M, Papamokos E, Huber R
TitleThree-dimensional structure of the complex between pancreatic secretory trypsin inhibitor (Kazal type) and trypsinogen at 1.8 A resolution. Structure solution, crystallographic refinement and preliminary structural interpretation.
Related PDB1tgs
[6]
CommentsX-ray crystallography
JournalActa Crystallogr B
Year1983
Volume39
Pages480-90
AuthorsMarquart M, Walter J, Deisenhofer J, Bode W, Huber R
TitleThe geometry of the reactive site and of the peptide groups in trypsin, trypsinogen and its complexes with inhibitors.
Related PDB1tpa,1tpo,1tpp,2ptc,2tgp,3ptb,3tpi,1tpo,1tpp,2ptc,2tgp,3ptb,3tpi
[7]
CommentsNeutron Diffraction
PubMed ID6712567
JournalBasic Life Sci
Year1984
Volume27
Pages281-304
AuthorsKossiakoff AA
TitleUse of the neutron diffraction--H/D exchange technique to determine the conformational dynamics of trypsin.
Related PDB1ntp
[8]
CommentsX-ray crystallography
PubMed ID6207021
JournalEur J Biochem
Year1984
Volume144
Pages185-90
AuthorsBode W, Walter J, Huber R, Wenzel HR, Tschesche H
TitleThe refined 2.2-A (0.22-nm) X-ray crystal structure of the ternary complex formed by bovine trypsinogen, valine-valine and the Arg15 analogue of bovine pancreatic trypsin inhibitor.
Related PDB4tpi
[9]
CommentsX-ray crystallography
PubMed ID3032921
JournalJ Biochem (Tokyo)
Year1986
Volume100
Pages1637-46
AuthorsTsunogae Y, Tanaka I, Yamane T, Kikkawa J, Ashida T, Ishikawa C, Watanabe K, Nakamura S, Takahashi K
TitleStructure of the trypsin-binding domain of Bowman-Birk type protease inhibitor and its interaction with trypsin.
Related PDB1tab
[10]
CommentsX-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
Medline ID87292123
PubMed ID3112942
JournalScience
Year1987
Volume237
Pages905-9
AuthorsSprang S, Standing T, Fletterick RJ, Stroud RM, Finer-Moore J, Xuong NH, Hamlin R, Rutter WJ, Craik CS
TitleThe three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis.
Related PDB1trm,2trm
Related Swiss-protP00762
[11]
CommentsX-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
Medline ID88286735
PubMed ID3135412
JournalJ Mol Biol
Year1988
Volume200
Pages523-51
AuthorsRead RJ, James MN
TitleRefined crystal structure of Streptomyces griseus trypsin at 1.7 A resolution.
Related PDB1sgt
Related Swiss-protP00775
[12]
CommentsX-ray crystallography
PubMed ID2914611
JournalFEBS Lett
Year1989
Volume242
Pages285-92
AuthorsBode W, Greyling HJ, Huber R, Otlewski J, Wilusz T
TitleThe refined 2.0 A X-ray crystal structure of the complex formed between bovine beta-trypsin and CMTI-I, a trypsin inhibitor from squash seeds (Cucurbita maxima). Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes.
Related PDB1ppe
[13]
CommentsX-ray crystallography
PubMed ID2614845
JournalJ Mol Biol
Year1989
Volume210
Pages813-28
AuthorsBartunik HD, Summers LJ, Bartsch HH
TitleCrystal structure of bovine beta-trypsin at 1.5 A resolution in a crystal form with low molecular packing density. Active site geometry, ion pairs and solvent structure.
Related PDB1tld
[14]
PubMed ID2125487
JournalBiochemistry
Year1990
Volume29
Pages10018-22
AuthorsHynes TR, Randal M, Kennedy LA, Eigenbrot C, Kossiakoff AA
TitleX-ray crystal structure of the protease inhibitor domain of Alzheimer's amyloid beta-protein precursor.
[15]
CommentsX-ray crystallography
PubMed ID2252895
JournalBiochemistry
Year1990
Volume29
Pages8351-7
AuthorsMangel WF, Singer PT, Cyr DM, Umland TC, Toledo DL, Stroud RM, Pflugrath JW, Sweet RM
TitleStructure of an acyl-enzyme intermediate during catalysis: (guanidinobenzoyl)trypsin.
Related PDB1gbt
[16]
CommentsX-ray crystallography
PubMed ID2226434
JournalEur J Biochem
Year1990
Volume193
Pages175-82
AuthorsBode W, Turk D, Sturzebecher J
TitleGeometry of binding of the benzamidine- and arginine-based inhibitors N alpha-(2-naphthyl-sulphonyl-glycyl)-DL-p-amidinophenylalanyl-pipe ridine (NAPAP) and (2R,4R)-4-methyl-1-[N alpha-(3-methyl-1,2,3,4-tetrahydro-8- quinolinesulphonyl)-L-arginyl]-2-piperidine carboxylic acid (MQPA) to human alpha-thrombin. X-ray crystallographic determination of the NAPAP-trypsin complex and modeling of NAPAP-thrombin and MQPA-thrombin.
Related PDB1ppc
[17]
PubMed ID1988014
JournalBiochemistry
Year1991
Volume30
Pages133-43
AuthorsPrestrelski SJ, Byler DM, Liebman MN
TitleComparison of various molecular forms of bovine trypsin: correlation of infrared spectra with X-ray crystal structures.
[18]
CommentsX-ray crystallography
PubMed ID1879520
JournalFEBS Lett
Year1991
Volume287
Pages133-8
AuthorsTurk D, Sturzebecher J, Bode W
TitleGeometry of binding of the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino phenylalanine to thrombin and trypsin. X-ray crystal structures of their trypsin complexes and modeling of their thrombin complexes.
Related PDB1pph
[19]
PubMed ID1794983
JournalJ Biochem (Tokyo)
Year1991
Volume110
Pages945-50
AuthorsYamane T, Kobuke M, Tsutsui H, Toida T, Suzuki A, Ashida T, Kawata Y, Sakiyama F
TitleCrystal structure of Streptomyces erythraeus trypsin at 2.7 A resolution.
[20]
PubMed ID1714504
JournalJ Mol Biol
Year1991
Volume220
Pages757-70
AuthorsHousset D, Kim KS, Fuchs J, Woodward C, Wlodawer A
TitleCrystal structure of a Y35G mutant of bovine pancreatic trypsin inhibitor.
[21]
PubMed ID1904942
JournalJ Mol Biol
Year1991
Volume219
Pages511-23
AuthorsWilke ME, Higaki JN, Craik CS, Fletterick RJ
TitleCrystal structure of rat trypsin-S195C at -150 degrees C. Analysis of low activity of recombinant and semisynthetic thiol proteases.
[22]
PubMed ID2051486
JournalJ Mol Biol
Year1991
Volume219
Pages525-32
AuthorsWilke ME, Higaki JN, Craik CS, Fletterick RJ
TitleCrystallographic analysis of trypsin-G226A. A specificity pocket mutant of rat trypsin with altered binding and catalysis.
[23]
CommentsX-RAY CRYSTALLOGRAPHY (1.59 ANGSTROMS).
Medline ID91351998
PubMed ID1881877
JournalProteins
Year1991
Volume10
Pages171-87
AuthorsEarnest T, Fauman E, Craik CS, Stroud R
Title1.59 A structure of trypsin at 120 K: comparison of low temperature and room temperature structures.
Related PDB1dpo
Related Swiss-protP00763
[24]
CommentsX-ray crystallography
PubMed ID1554694
JournalBiochemistry
Year1992
Volume31
Pages3059-64
AuthorsMcGrath ME, Vasquez JR, Craik CS, Yang AS, Honig B, Fletterick RJ
TitlePerturbing the polar environment of Asp102 in trypsin: consequences of replacing conserved Ser214.
Related PDB1anb,1anc
[25]
CommentsX-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
Medline ID92201369
PubMed ID1551419
JournalFEBS Lett
Year1992
Volume297
Pages143-6
AuthorsHuang Q, Liu S, Tang Y, Zeng F, Qian R
TitleAmino acid sequencing of a trypsin inhibitor by refined 1.6 A X-ray crystal structure of its complex with porcine beta-trypsin.
Related Swiss-protP00761
[26]
PubMed ID1383552
JournalJ Mol Biol
Year1992
Volume227
Pages757-75
AuthorsBerndt KD, Guntert P, Orbons LP, Wuthrich K
TitleDetermination of a high-quality nuclear magnetic resonance solution structure of the bovine pancreatic trypsin inhibitor and comparison with three crystal structures.
[27]
CommentsX-ray crystallography
PubMed ID1584773
JournalProc Natl Acad Sci U S A
Year1992
Volume89
Pages4407-11
AuthorsTakeuchi Y, Nonaka T, Nakamura KT, Kojima S, Miura K, Mitsui Y
TitleCrystal structure of an engineered subtilisin inhibitor complexed with bovine trypsin.
Related PDB2tld
[28]
CommentsX-ray crystallography
PubMed ID1557349
JournalProteins
Year1992
Volume12
Pages203-22
AuthorsFiner-Moore JS, Kossiakoff AA, Hurley JH, Earnest T, Stroud RM
TitleSolvent structure in crystals of trypsin determined by X-ray and neutron diffraction.
Related PDB4ptp,5ptp
[29]
PubMed ID1557350
JournalProteins
Year1992
Volume12
Pages223-36
AuthorsKossiakoff AA, Sintchak MD, Shpungin J, Presta LG
TitleAnalysis of solvent structure in proteins using neutron D2O-H2O solvent maps: pattern of primary and secondary hydration of trypsin.
[30]
CommentsX-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).
JournalActa Crystallogr D Biol Crystallogr
Year1993
Volume49
Pages318-30
AuthorsSmalas AO, Hordvik A
TitleStructure determination and refinement of benzamidine-inhibited trypsin from the North Atlantic salmon (Salmo salar) at 1.82-A resolution.
Related Swiss-protP35031
[31]
CommentsX-ray crystallography
PubMed ID8448149
JournalBiochemistry
Year1993
Volume32
Pages1914-9
AuthorsMcGrath ME, Haymore BL, Summers NL, Craik CS, Fletterick RJ
TitleStructure of an engineered, metal-actuated switch in trypsin.
Related PDB1and
[32]
PubMed ID8444191
JournalEur J Biochem
Year1993
Volume212
Pages549-55
AuthorsLin G, Bode W, Huber R, Chi C, Engh RA
TitleThe 0.25-nm X-ray structure of the Bowman-Birk-type inhibitor from mung bean in ternary complex with porcine trypsin.
[33]
CommentsX-ray crystallography
JournalJ Am Chem Soc
Year1993
Volume115
Pages12619-20
AuthorsLee AY, Hagihara M, Karmacharya R, Albers MW, Schreiber SL, Clardy J
TitleAtomic structure of the trypsin-cyclotheonamide A complex: lessons for the design of serine protease inhibitors.
Related PDB1tyn
[34]
CommentsX-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
Medline ID93187998
PubMed ID8445634
JournalJ Mol Biol
Year1993
Volume229
Pages1022-36
AuthorsHuang Q, Liu S, Tang Y
TitleRefined 1.6 A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex.
Related PDB1mct
Related Swiss-protP00761
[35]
CommentsX-ray crystallography
PubMed ID7683059
JournalJ Mol Biol
Year1993
Volume230
Pages919-33
AuthorsPerona JJ, Tsu CA, Craik CS, Fletterick RJ
TitleCrystal structures of rat anionic trypsin complexed with the protein inhibitors APPI and BPTI.
Related PDB1brb
[36]
CommentsX-ray crystallography
PubMed ID8478942
JournalJ Mol Biol
Year1993
Volume230
Pages934-49
AuthorsPerona JJ, Tsu CA, McGrath ME, Craik CS, Fletterick RJ
TitleRelocating a negative charge in the binding pocket of trypsin.
Related PDB1bra,1brc
[37]
PubMed ID8332590
JournalProtein Eng
Year1993
Volume6
Pages341-8
AuthorsRypniewski WR, Hastrup S, Betzel C, Dauter M, Dauter Z, Papendorf G, Branner S, Wilson KS
TitleThe sequence and X-ray structure of the trypsin from Fusarium oxysporum.
[38]
CommentsX-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
Medline ID95035057
PubMed ID7947985
JournalBiochim Biophys Acta
Year1994
Volume1209
Pages77-82
AuthorsHuang Q, Wang Z, Li Y, Liu S, Tang Y
TitleRefined 1.8 A resolution crystal structure of the porcine epsilon-trypsin.
Related PDB1ept
Related Swiss-protP00761
[39]
CommentsX-ray crystallography
PubMed ID9383379
JournalChem Biol
Year1994
Volume1
Pages113-7
AuthorsLee AY, Smitka TA, Bonjouklian R, Clardy J
TitleAtomic structure of the trypsin-A90720A complex: a unified approach to structure and function.
Related PDB1tps
[40]
CommentsX-ray crystallography
PubMed ID7798176
JournalJ Biochem (Tokyo)
Year1994
Volume116
Pages18-25
AuthorsLi Y, Huang Q, Zhang S, Liu S, Chi C, Tang Y
TitleStudies on an artificial trypsin inhibitor peptide derived from the mung bean trypsin inhibitor: chemical synthesis, refolding, and crystallographic analysis of its complex with trypsin.
Related PDB1g9i,1smf
[41]
CommentsX-ray crystallography
PubMed ID7634078
JournalNat Struct Biol
Year1994
Volume1
Pages735-43
AuthorsKurinov IV, Harrison RW
TitlePrediction of new serine proteinase inhibitors.
Related PDB1tng,1tnh,1tni,1tnj,1tnk,1tnl
[42]
CommentsX-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).
Medline ID95148588
PubMed ID7846025
JournalProteins
Year1994
Volume20
Pages149-66
AuthorsSmalas AO, Heimstad ES, Hordvik A, Willassen NP, Male R
TitleCold adaption of enzymes: structural comparison between salmon and bovine trypsins.
Related PDB1tbs,2tbs
Related Swiss-protP35031
[43]
CommentsX-ray crystallography
JournalActa Crystallogr D Biol Crystallogr
Year1995
Volume51
Pages725-30
AuthorsBerglund GI, Smalas AO, Hordvik A, Willassen NP
TitleStructure of anionic salmon trypsin in a second crystal form
Related PDB1bit
[44]
CommentsX-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS).
JournalActa Crystallogr D Biol Crystallogr
Year1995
Volume51
Pages73-84
AuthorsRypniewski WR, Dambmann C, von der Osten C, Dauter M, Wilson K.S
TitleStructure of inhibited trypsin from Fusarium oxysporum at 1.55 A.
Related PDB1try
Related Swiss-protP35049
[45]
PubMed ID7503719
JournalBiochem Biophys Res Commun
Year1995
Volume217
Pages437-44
AuthorsAscenzi P, Balliano G, Milla P, Ferraccioli R, Sartori P, Djinovic-Carugo K, Bolognesi M
TitleInhibition of bovine beta-trypsin by the active site titrant N alpha-(N,N-dimethylcarbamoyl)-alpha-azaornithine p-nitrophenyl ester: a kinetic and X-ray crystallographic study.
[46]
CommentsX-ray crystallography
PubMed ID7599119
JournalBiochemistry
Year1995
Volume34
Pages8264-80
AuthorsKatz BA, Finer-Moore J, Mortezaei R, Rich DH, Stroud RM
TitleEpiselection: novel Ki approximately nanomolar inhibitors of serine proteases selected by binding or chemistry on an enzyme surface.
Related PDB1btw,1btx,1bty,1btz
[47]
CommentsX-ray crystallography
PubMed ID7548040
JournalBiochemistry
Year1995
Volume34
Pages12849-53
AuthorsOdagaki Y, Nakai H, Senokuchi K, Kawamura M, Hamanaka N, Nakamura M, Tomoo K, Ishida T
TitleUnique binding of a novel synthetic inhibitor, N-[3-[4-[4-(amidinophenoxy)carbonyl]phenyl]-2-methyl-2-propenoyl]- N-allylglycine methanesulfonate, to bovine trypsin, revealed by the crystal structure of the complex.
Related PDB1btp
[48]
PubMed ID7703351
JournalBiophys Chem
Year1995
Volume54
Pages75-81
AuthorsCasale E, Collyer C, Ascenzi P, Balliano G, Milla P, Viola F, Fasano M, Menegatti E, Bolognesi M
TitleInhibition of bovine beta-trypsin, human alpha-thrombin and porcine pancreatic beta-kallikrein-B by 4',6-diamidino-2-phenylindole, 6-amidinoindole and benzamidine: a comparative thermodynamic and X-ray structural study.
[49]
PubMed ID7821429
JournalFEBS Lett
Year1995
Volume358
Pages53-6
AuthorsSartori P, Djinovic Carugo K, Ferraccioli R, Balliano G, Milla P, Ascenzi P, Bolognesi M
TitleActive site titration of bovine beta-trypsin by N alpha-(N,N-dimethylcarbamoyl)-alpha-aza-lysine p-nitrophenyl ester: kinetic and crystallographic analysis.
[50]
CommentsX-ray crystallography
PubMed ID7498454
JournalFEBS Lett
Year1995
Volume375
Pages103-7
AuthorsStubbs MT, Huber R, Bode W
TitleCrystal structures of factor Xa specific inhibitors in complex with trypsin: structural grounds for inhibition of factor Xa and selectivity against thrombin.
Related PDB1mts,1mtu,1mtv,1mtw
[51]
PubMed ID8749303
JournalJ Biochem (Tokyo)
Year1995
Volume118
Pages882-94
AuthorsYamane T, Iwasaki A, Suzuki A, Ashida T, Kawata Y
TitleCrystal structure of Streptomyces erythraeus trypsin at 1.9 A resolution.
[52]
PubMed ID7616573
JournalJ Mol Biol
Year1995
Volume250
Pages553-70
AuthorsMcDowell RS, Kossiakoff AA
TitleA comparison of neutron diffraction and molecular dynamics structures: hydroxyl group and water molecule orientations in trypsin.
[53]
CommentsX-ray crystallography
PubMed ID7795518
JournalProtein Sci
Year1995
Volume4
Pages337-60
AuthorsPerona JJ, Craik CS
TitleStructural basis of substrate specificity in the serine proteases.
Related PDB1amh
[54]
PubMed ID9092457
JournalBasic Life Sci
Year1996
Volume64
Pages273-87
AuthorsMcDowell RS, Kossiakoff AA
TitleHydroxyl and water molecule orientations in trypsin: comparison to molecular dynamic structures.
[55]
CommentsX-ray crystallography
PubMed ID8605148
JournalBiochemistry
Year1996
Volume35
Pages3147-55
AuthorsBertrand JA, Oleksyszyn J, Kam CM, Boduszek B, Presnell S, Plaskon RR, Suddath FL, Powers JC, Williams LD
TitleInhibition of trypsin and thrombin by amino(4-amidinophenyl)methanephosphonate diphenyl ester derivatives: X-ray structures and molecular models.
Related PDB1max,1may
[56]
CommentsX-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
Medline ID96214506
PubMed ID8634241
JournalBiochemistry
Year1996
Volume35
Pages5999-6009
AuthorsBrinen LS, Willett WS, Craik CS, Fletterick RJ
TitleX-ray structures of a designed binding site in trypsin show metal-dependent geometry.
Related PDB1slu,1slv,1slw,1slx
Related Swiss-protP00763
[57]
PubMed ID8960786
JournalClin Chim Acta
Year1996
Volume256
Pages37-51
AuthorsFinotti P
TitleSeparation by heparin-affinity chromatography of catalytically active and inactive forms of trypsin which retain the (Na-K)ATPase stimulating property.
[58]
CommentsX-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), AND PHOSPHORYLATION.
Medline ID96266496
PubMed ID8683601
JournalJ Mol Biol
Year1996
Volume259
Pages995-1010
AuthorsGaboriaud C, Serre L, Guy-Crotte O, Forest E, Fontecilla-Camps JC
TitleCrystal structure of human trypsin 1: unexpected phosphorylation of Tyr151.
Related PDB1trn
Related Swiss-protP07477
[59]
CommentsX-ray crystallography
PubMed ID8845765
JournalProtein Sci
Year1996
Volume5
Pages752-8
AuthorsKurinov IV, Harrison RW
TitleTwo crystal structures of the leupeptin-trypsin complex.
Related PDB1jrs,1jrt
[60]
CommentsX-ray crystallography
JournalActa Crystallogr D Biol Crystallogr
Year1997
Volume53
Pages311-5
AuthorsJohnson A, Krishnaswamy S, Sundaram PV, Pattabhi V
TitleThe First Structure at 1.8 A Resolution of an Active Autolysate Form of Porcine [alpha]-Trysoin.
Related PDB1aks
[61]
CommentsX-ray crystallography
PubMed ID9341205
JournalBiochemistry
Year1997
Volume36
Pages13180-6
AuthorsLee SL, Alexander RS, Smallwood A, Trievel R, Mersinger L, Weber PC, Kettner C
TitleNew inhibitors of thrombin and other trypsin-like proteases: hydrogen bonding of an aromatic cyano group with a backbone amide of the P1 binding site replaces binding of a basic side chain.
Related PDB1auj
[62]
CommentsX-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF COMPLEX WITH LDTI.
Medline ID97390427
PubMed ID9242660
JournalJ Biol Chem
Year1997
Volume272
Pages19931-7
AuthorsStubbs MT, Morenweiser R, Sturzebecher J, Bauer M, Bode W, Huber R, Piechottka GP, Matschiner G, Sommerhoff CP, Fritz H, Auerswald EA
TitleThe three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition.
Related PDB1ldt
Related Swiss-protP00761
[63]
CommentsX-ray crystallography
PubMed ID9199408
JournalJ Mol Biol
Year1997
Volume269
Pages395-407
AuthorsBurgering MJ, Orbons LP, van der Doelen A, Mulders J, Theunissen HJ, Grootenhuis PD, Bode W, Huber R, Stubbs MT
TitleThe second Kunitz domain of human tissue factor pathway inhibitor: cloning, structure determination and interaction with factor Xa.
Related PDB1tfx
[64]
CommentsX-ray crystallography
PubMed ID9300481
JournalProtein Sci
Year1997
Volume6
Pages1806-24
AuthorsScheidig AJ, Hynes TR, Pelletier LA, Wells JA, Kossiakoff AA
TitleCrystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities.
Related PDB1taw
[65]
CommentsX-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF COMPLEX WITH LDTI.
Medline ID98046095
PubMed ID9384562
JournalStructure
Year1997
Volume5
Pages1465-74
AuthorsDi Marco S, Priestle JP
TitleStructure of the complex of leech-derived tryptase inhibitor (LDTI) with trypsin and modeling of the LDTI-tryptase system.
Related PDB1an1
Related Swiss-protP00761
[66]
CommentsX-ray crystallography
PubMed ID9757092
JournalActa Crystallogr D Biol Crystallogr
Year1998
Volume54
Pages780-98
AuthorsSchroder HK, Willassen NP, Smalas AO
TitleStructure of a non-psychrophilic trypsin from a cold-adapted fish species.
Related PDB1a0j
[67]
CommentsX-ray crystallography
PubMed ID9724521
JournalBiochemistry
Year1998
Volume37
Pages12094-103
AuthorsKrishnan R, Zhang E, Hakansson K, Arni RK, Tulinsky A, Lim-Wilby MS, Levy OE, Semple JE, Brunck TK
TitleHighly selective mechanism-based thrombin inhibitors: structures of thrombin and trypsin inhibited with rigid peptidyl aldehydes.
Related PDB1yyy,1zzz
[68]
CommentsX-ray crystallography
PubMed ID9836602
JournalBiochemistry
Year1998
Volume37
Pages17068-81
AuthorsPresnell SR, Patil GS, Mura C, Jude KM, Conley JM, Bertrand JA, Kam CM, Powers JC, Williams LD
TitleOxyanion-mediated inhibition of serine proteases.
Related PDB1bju,1bjv
[69]
CommentsX-ray crystallography
PubMed ID9920392
JournalBiochim Biophys Acta
Year1998
Volume1429
Pages142-50
AuthorsZhu G, Huang Q, Wang Z, Qian M, Jia Y, Tang Y
TitleX-ray studies on two forms of bovine beta-trypsin crystals in neat cyclohexane.
Related PDB1tio,2tio
[70]
CommentsX-ray crystallography
PubMed ID9760170
JournalEur J Biochem
Year1998
Volume256
Pages317-24
AuthorsHelland R, Leiros I, Berglund GI, Willassen NP, Smalas AO
TitleThe crystal structure of anionic salmon trypsin in complex with bovine pancreatic trypsin inhibitor.
Related PDB1bzx
[71]
CommentsX-ray crystallography
JournalJ Am Chem Soc
Year1998
Volume120
Pages595-6
AuthorsSandler B, Murakami M, Clardy J
TitleAtomic Structure of the Trypsin-Aeruginosin 98-B Complex.
Related PDB1aq7
[72]
PubMed ID9876114
JournalJ Med Chem
Year1998
Volume41
Pages5445-56
AuthorsRenatus M, Bode W, Huber R, Sturzebecher J, Stubbs MT
TitleStructural and functional analyses of benzamidine-based inhibitors in complex with trypsin: implications for the inhibition of factor Xa, tPA, and urokinase.
[73]
CommentsX-ray crystallography
PubMed ID9466914
JournalJ Mol Biol
Year1998
Volume275
Pages347-63
AuthorsSong HK, Suh SW
TitleKunitz-type soybean trypsin inhibitor revisited: refined structure of its complex with porcine trypsin reveals an insight into the interaction between a homologous inhibitor from Erythrina caffra and tissue-type plasminogen activator.
Related PDB1avw,1avx
[74]
PubMed ID9495588
JournalJ Pept Res
Year1998
Volume51
Pages29-37
AuthorsBemquerer MP, Liria CW, Kitagawa K, Miranda MT, Tominaga M
TitleMixtures of trifluoroethanol or hexafluoroisopropanol and dimethylformamide are not of general applicability for peptide condensations catalyzed by trypsin.
[75]
CommentsX-ray crystallography
PubMed ID9468142
JournalNature
Year1998
Volume391
Pages608-12
AuthorsKatz BA, Clark JM, Finer-Moore JS, Jenkins TE, Johnson CR, Ross MJ, Luong C, Moore WR, Stroud RM
TitleDesign of potent selective zinc-mediated serine protease inhibitors.
Related PDB1c1n,1c1o,1c1p,1c1q,1c1r,1c1s,1c1t,1c2d,1c2e,1c2f,1c2g,1c2h,1c2i,1c2j,1c2k,1c2l,1c2m,1xuf,1xug,1xuh,1xui,1xuj,1xuk
[76]
CommentsX-ray crystallography
PubMed ID9707558
JournalProc Natl Acad Sci U S A
Year1998
Volume95
Pages9813-8
AuthorsHopfner KP, Kopetzki E, Kresse GB, Bode W, Huber R, Engh RA
TitleNew enzyme lineages by subdomain shuffling.
Related PDB1fxy
[77]
PubMed ID9749919
JournalProtein Eng
Year1998
Volume11
Pages669-73
AuthorsHung SH, Hedstrom L
TitleConverting trypsin to elastase: substitution of the S1 site and adjacent loops reconstitutes esterase specificity but not amidase activity.
[78]
CommentsX-ray crystallography
PubMed ID10089404
JournalActa Crystallogr D Biol Crystallogr
Year1999
Volume55
Pages139-48
AuthorsHelland R, Berglund GI, Otlewski J, Apostoluk W, Andersen OA, Willassen NP, Smalas AO
TitleHigh-resolution structures of three new trypsin-squash-inhibitor complexes: a detailed comparison with other trypsins and their complexes.
Related PDB2btc,2sta,2stb
[79]
CommentsX-ray crystallography
PubMed ID10531473
JournalActa Crystallogr D Biol Crystallogr
Year1999
Volume55
Pages1785-91
AuthorsRecacha R, Carson M, Costanzo MJ, Maryanoff B, DeLucas LJ, Chattopadhyay D
TitleStructure of the RWJ-51084-bovine pancreatic beta-trypsin complex at 1.8 A.
Related PDB1qcp
[80]
CommentsX-ray crystallography
PubMed ID10417407
JournalActa Crystallogr D Biol Crystallogr
Year1999
Volume55
Pages1395-404
AuthorsWhitlow M, Arnaiz DO, Buckman BO, Davey DD, Griedel B, Guilford WJ, Koovakkat SK, Liang A, Mohan R, Phillips GB, Seto M, Shaw KJ, Xu W, Zhao Z, Light DR, Morrissey MM
TitleCrystallographic analysis of potent and selective factor Xa inhibitors complexed to bovine trypsin.
Related PDB1qa0,1qb1,1qb6,1qb9,1qbn,1qbo
[81]
PubMed ID9989934
JournalArch Biochem Biophys
Year1999
Volume362
Pages254-64
AuthorsKaslik G, Westler WM, Graf L, Markley JL
TitleProperties of the His57-Asp102 dyad of rat trypsin D189S in the zymogen, activated enzyme, and alpha1-proteinase inhibitor complexed forms.
[82]
CommentsX-ray crystallography
PubMed ID10561533
JournalBiochim Biophys Acta
Year1999
Volume1435
Pages7-21
AuthorsJohnson A, Gautham N, Pattabhi V
TitleCrystal structure at 1.63 A resolution of the native form of porcine beta-trypsin: revealing an acetate ion binding site and functional water network.
Related PDB1qqu
[83]
PubMed ID10429182
JournalEur J Biochem
Year1999
Volume263
Pages20-6
AuthorsSzabo E, Bocskei Z, Naray-Szabo G, Graf L
TitleThe three-dimensional structure of Asp189Ser trypsin provides evidence for an inherent structural plasticity of the protease.
[84]
PubMed ID10641732
JournalFree Radic Biol Med
Year1999
Volume27
Pages1378-85
AuthorsFinotti P, Corvaja C, Pagetta A
TitleDifferential mechanisms for structural and functional alterations of trypsin by heparin, evidence for a specific, radical-generating mechanism at low heparin concentrations.
[85]
PubMed ID10517520
JournalInt J Biol Macromol
Year1999
Volume26
Pages135-44
AuthorsFinotti P, Pagetta A, Corvaja C
TitleRole of reducing terminals in unfractionated and low-molecular-mass heparins in causing free radical generation and loss of structure and activity of trypsin.
[86]
PubMed ID10373424
JournalJ Biol Chem
Year1999
Volume274
Pages18231-6
AuthorsLin CY, Anders J, Johnson M, Sang QA, Dickson RB
TitleMolecular cloning of cDNA for matriptase, a matrix-degrading serine protease with trypsin-like activity.
[87]
CommentsX-ray crystallography
PubMed ID10447205
JournalJ Biomol Struct Dyn
Year1999
Volume16
Pages1219-24
AuthorsZhu Y, Huang Q, Chi C
TitleCrystal structure of mung bean inhibitor lysine active fragment complex with bovine beta-trypsin at 1.8A resolution.
Related PDB1sbw
[88]
CommentsX-ray crystallography
PubMed ID10222201
JournalJ Mol Biol
Year1999
Volume287
Pages923-42
AuthorsHelland R, Otlewski J, Sundheim O, Dadlez M, Smalas AO
TitleThe crystal structures of the complexes between bovine beta-trypsin and ten P1 variants of BPTI.
Related PDB3btd,3bte,3btf,3btg,3bth,3btk,3btm,3btq,3btt,3btw
[89]
CommentsX-ray crystallography
PubMed ID10390350
JournalJ Mol Biol
Year1999
Volume290
Pages525-33
AuthorsLuckett S, Garcia RS, Barker JJ, Konarev AV, Shewry PR, Clarke AR, Brady RL
TitleHigh-resolution structure of a potent, cyclic proteinase inhibitor from sunflower seeds.
Related PDB1sfi
[90]
PubMed ID10356328
JournalJ Mol Biol
Year1999
Volume289
Pages547-64
AuthorsNakasako M
TitleLarge-scale networks of hydration water molecules around bovine beta-trypsin revealed by cryogenic X-ray crystal structure analysis.
[91]
CommentsX-ray crystallography
PubMed ID10512718
JournalJ Mol Biol
Year1999
Volume293
Pages93-106
AuthorsRester U, Bode W, Moser M, Parry MA, Huber R, Auerswald E
TitleStructure of the complex of the antistasin-type inhibitor bdellastasin with trypsin and modelling of the bdellastasin-microplasmin system.
Related PDB1c9p,1c9t
[92]
PubMed ID10591040
JournalJ Nat Toxins
Year1999
Volume8
Pages363-84
AuthorsAzim MK, Grossmann JG, Zaidi ZH
TitlePredicted three-dimensional structural models of venom serine protease inhibitors and their interactions with trypsin and chymotrypsin.
[93]
CommentsX-ray crystallography
PubMed ID10524768
JournalJ Protein Chem
Year1999
Volume18
Pages505-9
AuthorsZhu Y, Huang Q, Qian M, Jia Y, Tang Y
TitleCrystal structure of the complex formed between bovine beta-trypsin and MCTI-A, a trypsin inhibitor of squash family, at 1.8-A resolution.
Related PDB1f2s,1mcu
[94]
CommentsX-ray crystallography
PubMed ID10210204
JournalProtein Sci
Year1999
Volume8
Pages253-8
AuthorsPasternak A, Ringe D, Hedstrom L
TitleComparison of anionic and cationic trypsinogens: the anionic activation domain is more flexible in solution and differs in its mode of BPTI binding in the crystal structure.
Related PDB3tgi,3tgj
[95]
CommentsX-ray crystallography
PubMed ID10651279
JournalProteins
Year1999
Volume37
Pages641-53
AuthorsOta N, Stroupe C, Ferreira-da-Silva JM, Shah SA, Mares-Guia M, Brunger AT
TitleNon-Boltzmann thermodynamic integration (NBTI) for macromolecular systems: relative free energy of binding of trypsin to benzamidine and benzylamine.
Related PDB1ce5,2bza
[96]
CommentsX-ray crystallography
PubMed ID10771427
JournalActa Crystallogr D Biol Crystallogr
Year2000
Volume56
Pages581-8
AuthorsRester U, Moser M, Huber R, Bode W
TitleL-Isoaspartate 115 of porcine beta-trypsin promotes crystallization of its complex with bdellastasin.
Related PDB1eja
[97]
CommentsX-ray crystallography
PubMed ID10779411
JournalChem Biol
Year2000
Volume7
Pages299-312
AuthorsKatz BA, Mackman R, Luong C, Radika K, Martelli A, Sprengeler PA, Wang J, Chan H, Wong L
TitleStructural basis for selectivity of a small molecule, S1-binding, submicromolar inhibitor of urokinase-type plasminogen activator.
Related PDB1c5p,1c5q,1c5r,1c5s,1c5t,1c5u,1c5v
[98]
PubMed ID11126764
JournalComp Biochem Physiol B Biochem Mol Biol
Year2000
Volume127
Pages337-46
AuthorsSekizaki H, Itoh K, Murakami M, Toyota E, Tanizawa K
TitleAnionic trypsin from chum salmon: activity with p-amidinophenyl ester and comparison with bovine and Streptomyces griseus trypsins.
[99]
CommentsX-ray crystallography
PubMed ID10966741
JournalJ Med Chem
Year2000
Volume43
Pages3226-32
AuthorsMaignan S, Guilloteau JP, Pouzieux S, Choi-Sledeski YM, Becker MR, Klein SI, Ewing WR, Pauls HW, Spada AP, Mikol V
TitleCrystal structures of human factor Xa complexed with potent inhibitors.
Related PDB1f0t,1f0u
[100]
CommentsX-ray crystallography
PubMed ID10843853
JournalJ Mol Biol
Year2000
Volume299
Pages993-1003
AuthorsGillmor SA, Takeuchi T, Yang SQ, Craik CS, Fletterick RJ
TitleCompromise and accommodation in ecotin, a dimeric macromolecular inhibitor of serine proteases.
Related PDB1ezs,1ezu
[101]
CommentsX-ray crystallography
PubMed ID10926503
JournalJ Mol Biol
Year2000
Volume301
Pages205-17
AuthorsGrzesiak A, Helland R, Smalas AO, Krowarsch D, Dadlez M, Otlewski J
TitleSubstitutions at the P(1) position in BPTI strongly affect the association energy with serine proteinases.
Related PDB1ejm
[102]
CommentsX-ray crystallography
PubMed ID10772864
JournalJ Mol Biol
Year2000
Volume298
Pages477-91
AuthorsKoepke J, Ermler U, Warkentin E, Wenzl G, Flecker P
TitleCrystal structure of cancer chemopreventive Bowman-Birk inhibitor in ternary complex with bovine trypsin at 2.3 A resolution. Structural basis of Janus-faced serine protease inhibitor specificity.
Related PDB1d6r
[103]
CommentsX-ray crystallography
PubMed ID11057674
JournalNature
Year2000
Volume407
Pages923-6
AuthorsHuntington JA, Read RJ, Carrell RW
TitleStructure of a serpin-protease complex shows inhibition by deformation.
Related PDB1ezx
[104]
PubMed ID10944388
JournalProteins
Year2000
Volume41
Pages8-16
AuthorsPletnev VZ, Zamolodchikova TS, Pangborn WA, Duax WL
TitleCrystal structure of bovine duodenase, a serine protease, with dual trypsin and chymotrypsin-like specificities.
[105]
CommentsX-ray crystallography
PubMed ID11679713
JournalActa Crystallogr D Biol Crystallogr
Year2001
Volume57
Pages1506-12
AuthorsDeepthi S, Johnson A, Pattabhi V
TitleStructures of porcine beta-trypsin-detergent complexes: the stabilization of proteins through hydrophilic binding of polydocanol.
Related PDB1fmg,1fn6,1fni
[106]
CommentsX-ray crystallography
PubMed ID11264577
JournalActa Crystallogr D Biol Crystallogr
Year2001
Volume57
Pages488-97
AuthorsLeiros HK, McSweeney SM, Smalas AO
TitleAtomic resolution structures of trypsin provide insight into structural radiation damage.
Related PDB1hj8,1hj9
[107]
CommentsX-ray crystallography
PubMed ID11134922
JournalActa Crystallogr D Biol Crystallogr
Year2001
Volume57
Pages8-19
AuthorsRypniewski WR, Ostergaard PR, Norregaard-Madsen M, Dauter M, Wilson KS
TitleFusarium oxysporum trypsin at atomic resolution at 100 and 283 K: a study of ligand binding.
Related PDB1fn8,1fy4,1fy5,1gdn,1gdq,1gdu
[108]
PubMed ID11371189
JournalBiochemistry
Year2001
Volume40
Pages6275-83
AuthorsPeterson FC, Gordon NC, Gettins PG
TitleHigh-level bacterial expression and 15N-alanine-labeling of bovine trypsin. Application to the study of trypsin-inhibitor complexes and trypsinogen activation by NMR spectroscopy.
[109]
PubMed ID11731085
JournalBiochim Biophys Acta
Year2001
Volume1568
Pages53-9
AuthorsPark H, Chi YM
TitleThe enhancement of electrostriction caused by lowering the solvent dielectric constant leads to the decrease of activation energy in trypsin catalysis.
[110]
CommentsX-ray crystallography
PubMed ID11731301
JournalChem Biol
Year2001
Volume8
Pages1107-21
AuthorsKatz BA, Sprengeler PA, Luong C, Verner E, Elrod K, Kirtley M, Janc J, Spencer JR, Breitenbucher JG, Hui H, McGee D, Allen D, Martelli A, Mackman RL
TitleEngineering inhibitors highly selective for the S1 sites of Ser190 trypsin-like serine protease drug targets.
Related PDB1gj6
[111]
PubMed ID11508835
JournalEur Biophys J
Year2001
Volume30
Pages163-70
AuthorsCaracciolo G, Amiconi G, Bencivenni L, Boumis G, Caminiti R, Finocchiaro E, Maras B, Paolinelli C, Congiu Castellano A
TitleConformational study of proteins by SAXS and EDXD: the case of trypsin and trypsinogen.
[112]
PubMed ID11226886
JournalJ Biochem (Tokyo)
Year2001
Volume129
Pages455-60
AuthorsTomoo K, Satoh K, Tsuda Y, Wanaka K, Okamoto S, Hijikata-Okunomiya A, Okada Y, Ishida T
TitleBinding diversity of a noncovalent-type low-molecular-weight serine protease inhibitor and function of a catalytic water molecule: X-ray crystal structure of PKSI-527--inhibited trypsin.
[113]
PubMed ID11312265
JournalJ Biol Chem
Year2001
Volume276
Pages24574-80
AuthorsSzilagyi L, Kenesi E, Katona G, Kaslik G, Juhasz G, Graf L
TitleComparative in vitro studies on native and recombinant human cationic trypsins. Cathepsin B is a possible pathological activator of trypsinogen in pancreatitis.
[114]
CommentsX-ray crystallography
PubMed ID11676542
JournalJ Mol Biol
Year2001
Volume313
Pages593-614
AuthorsDullweber F, Stubbs MT, Musil D, Sturzebecher J, Klebe G
TitleFactorising ligand affinity: a combined thermodynamic and crystallographic study of trypsin and thrombin inhibition.
Related PDB1k1i,1k1j,1k1l,1k1m,1k1n,1k1o,1k1p
[115]
CommentsX-ray crystallography
PubMed ID11292354
JournalJ Mol Biol
Year2001
Volume307
Pages1451-86
AuthorsKatz BA, Elrod K, Luong C, Rice MJ, Mackman RL, Sprengeler PA, Spencer J, Hataye J, Janc J, Link J, Litvak J, Rai R, Rice K, Sideris S, Verner E, Young W
TitleA novel serine protease inhibition motif involving a multi-centered short hydrogen bonding network at the active site.
Related PDB1ghz,1gi0,1gi1,1gi2,1gi3,1gi4,1gi5,1gi6
[116]
CommentsX-ray crystallography
PubMed ID11152605
JournalJ Mol Biol
Year2001
Volume305
Pages471-9
AuthorsToyota E, Ng KK, Sekizaki H, Itoh K, Tanizawa K, James MN
TitleX-ray crystallographic analyses of complexes between bovine beta-trypsin and Schiff base copper(II) or iron(III) chelates.
Related PDB1g3b,1g3c,1g3d,1g3e
[117]
CommentsX-ray crystallography
PubMed ID11685246
JournalNat Struct Biol
Year2001
Volume8
Pages979-83
AuthorsYe S, Cech AL, Belmares R, Bergstrom RC, Tong Y, Corey DR, Kanost MR, Goldsmith EJ
TitleThe structure of a Michaelis serpin-protease complex.
Related PDB1i99,1k9o
[118]
CommentsX-ray crystallography
PubMed ID11420435
JournalProtein Sci
Year2001
Volume10
Pages1331-42
AuthorsPasternak A, White A, Jeffery CJ, Medina N, Cahoon M, Ringe D, Hedstrom L
TitleThe energetic cost of induced fit catalysis: Crystal structures of trypsinogen mutants with enhanced activity and inhibitor affinity.
Related PDB1fy8,1f5r,1f7z,3tgk
[119]
CommentsX-ray crystallography
PubMed ID11342132
JournalStructure (Camb)
Year2001
Volume9
Pages29-37
AuthorsNar H, Bauer M, Schmid A, Stassen JM, Wienen W, Priepke HW, Kauffmann IK, Ries UJ, Hauel NH
TitleStructural basis for inhibition promiscuity of dual specific thrombin and factor Xa blood coagulation inhibitors.
Related PDB1g36,1oyq
[120]
PubMed ID11779198
JournalBiochem Biophys Res Commun
Year2002
Volume290
Pages494-7
AuthorsMarana SR, Lopes AR, Juliano L, Juliano MA, Ferreira C, Terra WR
TitleSubsites of trypsin active site favor catalysis or substrate binding.
[121]
PubMed ID11921406
JournalChembiochem
Year2002
Volume3
Pages246-9
AuthorsStubbs MT, Reyda S, Dullweber F, Moller M, Klebe G, Dorsch D, Mederski WW, Wurziger H
TitlepH-dependent binding modes observed in trypsin crystals: lessons for structure-based drug design.
Related PDB1ql7,1ql8,1ql9
[122]
CommentsX-ray crystallography
PubMed ID12061878
JournalJ Med Chem
Year2002
Volume45
Pages2749-69
AuthorsMatter H, Defossa E, Heinelt U, Blohm PM, Schneider D, Muller A, Herok S, Schreuder H, Liesum A, Brachvogel V, Lonze P, Walser A, Al-Obeidi F, Wildgoose P
TitleDesign and quantitative structure-activity relationship of 3-amidinobenzyl-1H-indole-2-carboxamides as potent, nonchiral, and selective inhibitors of blood coagulation factor Xa.
Related PDB1lqe
[123]
PubMed ID11827488
JournalJ Mol Biol
Year2002
Volume315
Pages1209-18
AuthorsKatona G, Berglund GI, Hajdu J, Graf L, Szilagyi L
TitleCrystal structure reveals basis for the inhibitor resistance of human brain trypsin.
[124]
PubMed ID12445776
JournalJ Mol Biol
Year2002
Volume324
Pages391-7
AuthorsToyota E, Ng KK, Kuninaga S, Sekizaki H, Itoh K, Tanizawa K, James MN
TitleCrystal structure and nucleotide sequence of an anionic trypsin from chum salmon (Oncorhynchus keta) in comparison with Atlantic salmon (Salmo salar) and bovine trypsin.
[125]
PubMed ID12705877
JournalBiochem Biophys Res Commun
Year2003
Volume304
Pages18-21
AuthorsKotorman M, Laczko I, Szabo A, Simon LM
TitleEffects of Ca2+ on catalytic activity and conformation of trypsin and alpha-chymotrypsin in aqueous ethanol.
[126]
CommentsX-ray crystallography
PubMed ID12885239
JournalBiochemistry
Year2003
Volume42
Pages9060-6
AuthorsPage MJ, Wong SL, Hewitt J, Strynadka NC, MacGillivray RT
TitleEngineering the primary substrate specificity of Streptomyces griseus trypsin.
Related PDB1os8,1oss
[127]
PubMed ID12837799
JournalJ Bacteriol
Year2003
Volume185
Pages4233-42
AuthorsSudom A, Walters R, Pastushok L, Goldie D, Prasad L, Delbaere LT, Goldie H
TitleMechanisms of activation of phosphoenolpyruvate carboxykinase from Escherichia coli by Ca2+ and of desensitization by trypsin.
[128]
CommentsX-ray crystallography
PubMed ID12860985
JournalJ Biol Chem
Year2003
Volume278
Pages37881-7
AuthorsDementiev A, Simonovic M, Volz K, Gettins PG
TitleCanonical inhibitor-like interactions explain reactivity of alpha1-proteinase inhibitor Pittsburgh and antithrombin with proteinases.
Related PDB1oph
[129]
CommentsX-ray crystallography
PubMed ID12937176
JournalJ Biol Chem
Year2003
Volume278
Pages43357-62
AuthorsSchmidt A, Jelsch C, Ostergaard P, Rypniewski W, Lamzin VS
TitleTrypsin revisited: crystallography AT (SUB) atomic resolution and quantum chemistry revealing details of catalysis.
Related PDB1ppz,1pq5,1pq7,1pq8,1pqa
[130]
CommentsX-ray crystallography
PubMed ID12930148
JournalJ Med Chem
Year2003
Volume46
Pages3865-76
AuthorsCostanzo MJ, Yabut SC, Almond HR Jr, Andrade-Gordon P, Corcoran TW, De Garavilla L, Kauffman JA, Abraham WM, Recacha R, Chattopadhyay D, Maryanoff BE
TitlePotent, small-molecule inhibitors of human mast cell tryptase. Antiasthmatic action of a dipeptide-based transition-state analogue containing a benzothiazole ketone.
Related PDB1nc6
[131]
PubMed ID14568540
JournalJ Mol Biol
Year2003
Volume333
Pages845-61
AuthorsHelland R, Czapinska H, Leiros I, Olufsen M, Otlewski J, Smalas AO
TitleStructural consequences of accommodation of four non-cognate amino acid residues in the S1 pocket of bovine trypsin and chymotrypsin.
[132]
CommentsX-ray crystallography
PubMed ID12742021
JournalJ Mol Biol
Year2003
Volume329
Pages93-120
AuthorsKatz BA, Elrod K, Verner E, Mackman RL, Luong C, Shrader WD, Sendzik M, Spencer JR, Sprengeler PA, Kolesnikov A, Tai VW, Hui HC, Breitenbucher JG, Allen D, Janc JW
TitleElaborate manifold of short hydrogen bond arrays mediating binding of active site-directed serine protease inhibitors.
Related PDB1o2h,1o2i,1o2j,1o2k,1o2l,1o2m,1o2n,1o2o,1o2p,1o2q,1o2r,1o2s,1o2t,1o2u,1o2v,1o2w,1o2x,1o2y,1o2z,1o30,1o31,1o32,1o33,1o34,1o35,1o36,1o37,1o38,1o39,1o3a,1o3b,1o3c,1o3d,1o3e,1o3f,1o3g,1o3h,1o3i,1o3j,1o3k,1o3l,1o3m,1o3n,1o3o
[133]
PubMed ID12527302
JournalJ Mol Biol
Year2003
Volume325
Pages963-77
AuthorsReyda S, Sohn C, Klebe G, Rall K, Ullmann D, Jakubke HD, Stubbs MT
TitleReconstructing the binding site of factor Xa in trypsin reveals ligand-induced structural plasticity.
Related PDB1j14,1j15,1j16,1j17
[134]
CommentsX-ray crystallography
PubMed ID12575941
JournalStructure (Camb)
Year2003
Volume11
Pages217-24
AuthorsRavelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S
TitleSpecific radiation damage can be used to solve macromolecular crystal structures.
Related PDB1n6x,1n6y
[135]
CommentsX-ray crystallography
PubMed ID14672690
JournalBiochem Biophys Res Commun
Year2004
Volume313
Pages8-16
AuthorsIbrahim BS, Pattabhi V
TitleCrystal structure of trypsin-turkey egg white inhibitor complex.
Related PDB1r0t
[136]
PubMed ID14729347
JournalJ Mol Biol
Year2004
Volume335
Pages1325-41
AuthorsRauh D, Klebe G, Stubbs MT
TitleUnderstanding protein-ligand interactions: the price of protein flexibility.
[137]
CommentsX-ray crystallography
PubMed ID15044735
JournalProtein Sci
Year2004
Volume13
Pages1056-70
AuthorsLeiros HK, Brandsdal BO, Andersen OA, Os V, Leiros I, Helland R, Otlewski J, Willassen NP, Smalas AO
TitleTrypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements.
Related PDB1utj,1utk,1utl,1utm,1utn,1uto,1utp,1utq

comments
The chains of the enzymes deposited in the following PDB structures are split into two or three fragments.
PDB;1aksA, Swiss-prot;TRYP_PIG;P00761, residues 9-133 (PDB;16-145)
PDB;1aksB, Swiss-prot;TRYP_PIG;P00761, residues 134-231 (PDB;146-245)
PDB;1eptA, Swiss-prot;TRYP_PIG;P00761, residues 9-51 (PDB;16-60)
PDB;1eptB, Swiss-prot;TRYP_PIG;P00761, residues 52-133 (PDB;61-145)
PDB;1eptC, Swiss-prot;TRYP_PIG;P00761, residues 132-231 (PDB;146-245)
This enzyme, trypsin, is a calssical serine protease, whose catalytic mechanism has been elucidated by various studies. This enzyme has got a catalytic triad, composed of Ser195/His57/Asp102.
According to the literature [10] and [53], the catalytic reaction proceeds as follows:
(1) His57 acts as a general base, by abstracting a proton from the hydroxyl group of Ser195. Here, Asp102 stabilizes the charged His57, and orients the conformation of His57 correctly (see [10]).
(2) Ser195, whose nucleophilicity was enhanced by His57, makes a nucleophilic attack on the carbonyl carbon atom of the scissile bond, peptide bond, leading to the formation of a tetrahedral acyl-intermediate.
(3) The oxyanion of the intermediate is stabilized by the mainchain amide nitrogens of Gly193 and Ser195.
(4) His57 acts as a general acid, by protonating to the newly formed amine group. This leads to the dissociation of the first product.
(5) A water molecule makes a nucleophilic attack on the acyl group, resulting in the deacylation.

createdupdated
2004-09-172009-02-26


Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2010)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)

© Computational Biology Research Center, AIST, 2004 All Rights Reserved.