EzCatDB: M00113

DB codeM00113
RLCP classification3.900.275800.990
CATH domainDomain 13.20.20.80Catalytic domain
Domain 22.60.40.1180
Domain 32.60.40.10
Domain 42.60.40.10
E.C.2.4.1.19
CSA1cdg,1eo7
MACiEM0045

CATH domainRelated DB codes (homologues)
2.60.40.10M00131,T00257,T00005,M00127,M00132,M00323,M00325,M00327,M00329,M00330,M00331,M00332,T00307,D00166,D00500,M00112,M00193,T00063,T00065,T00067,T00245
2.60.40.1180T00307,D00165,D00176,D00664,D00665,D00863,D00864,M00112,M00193,M00314,T00057,T00062,T00067
3.20.20.80S00202,S00210,S00748,S00906,S00907,S00911,S00912,S00915,M00134,M00160,D00479,S00204,S00205,S00206,S00207,S00203,S00208,S00209,S00211,S00213,S00214,T00307,D00165,D00166,D00169,D00176,D00501,D00502,D00503,D00844,D00861,D00864,M00026,M00112,M00193,M00346,T00057,T00062,T00063,T00066,T00067

Enzyme Name
Swiss-protKEGG

P05618P30920P43379P31797P26827
Protein nameCyclomaltodextrin glucanotransferaseCyclomaltodextrin glucanotransferaseCyclomaltodextrin glucanotransferaseCyclomaltodextrin glucanotransferaseCyclomaltodextrin glucanotransferasecyclomaltodextrin glucanotransferase
Bacillus macerans amylase
cyclodextrin glucanotransferase
alpha-cyclodextrin glucanotransferase
alpha-cyclodextrin glycosyltransferase
beta-cyclodextrin glucanotransferase
beta-cyclodextrin glycosyltransferase
gamma-cyclodextrin glycosyltransferase
cyclodextrin glycosyltransferase
cyclomaltodextrin glucotransferase
cyclomaltodextrin glycosyltransferase
konchizaimu
alpha-1,4-glucan 4-glycosyltransferase, cyclizing
BMA
CGTase
neutral-cyclodextrin glycosyltransferase
1,4-alpha-D-glucan 4-alpha-D-(1,4-alpha-D-glucano)-transferase(cyclizing)
SynonymsEC 2.4.1.19
Cyclodextrin-glycosyltransferase
CGTase
EC 2.4.1.19
Cyclodextrin-glycosyltransferase
CGTase
EC 2.4.1.19
Cyclodextrin-glycosyltransferase
CGTase
EC 2.4.1.19
Cyclodextrin-glycosyltransferase
CGTase
EC 2.4.1.19
Cyclodextrin-glycosyltransferase
CGTase


Swiss-prot:Accession NumberP05618P30920P43379P31797P26827
Entry nameCDGT_BACS0CDGT1_BACCICDGT2_BACCICDGT_BACSTCDGT_THETU
ActivityCyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.
SubunitMonomer.Monomer.Monomer.Monomer.
Subcellular locationSecreted (By similarity).Secreted (By similarity).Secreted (By similarity).Secreted (By similarity).Secreted.
CofactorBinds 2 calcium ions per subunit.Binds 2 calcium ions per subunit.Binds 2 calcium ions per subunit.Binds 2 calcium ions per subunit.Binds 2 calcium ions per subunit.


CofactorsSubstratesProductsintermediates
KEGG-idC00076C01935C00369C00973C01935

CompoundCalciumMaltodextrinStarchCyclomaltodextrinMaltodextrinTransition-state-1Covalent-bonded intermediate
Typedivalent metal (Ca2+, Mg2+)polysaccharidepolysaccharidepolysaccharidepolysaccharide

1d7fA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1d7fB01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1dedA01Bound:2x_CAAnalogue:ACRUnboundUnboundUnboundUnboundUnbound
1dedB01Bound:2x_CAAnalogue:ACRUnboundUnboundUnboundUnboundUnbound
1i75A01Bound:2x_CAAnalogue:NOJUnboundUnboundUnboundUnboundUnbound
1i75B01Bound:2x_CAAnalogue:NOJUnboundUnboundUnboundUnboundUnbound
1pamA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1pamB01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1ukqA01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-ACI-G6D-BGCUnbound
1ukqB01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-ACI-G6D-BGCUnbound
1uksA01Bound:2x_CAAnalogue:GLC-ACI-GLD-GALUnboundUnboundUnboundUnboundUnbound
1uksB01Bound:2x_CAAnalogue:GLC-ACI-GLD-GALUnboundUnboundUnboundUnboundUnbound
1uktA01Bound:2x_CAAnalogue:ACI-GLD-GLC-GALUnboundUnboundUnboundUnboundUnbound
1uktB01Bound:2x_CAAnalogue:ACI-GLD-GLC-GALUnboundUnboundUnboundUnboundUnbound
1v3jA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1v3jB01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1v3kA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1v3kB01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1v3lA01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-ACI-GLD-GAL(chain D)Unbound
1v3lB01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-ACI-GLD-GLC(chain E)Unbound
1v3mA01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-ACI-GLD-GAL(chain D)Unbound
1v3mB01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-ACI-GLD-GAL(chain H)Unbound
1cgtA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1cguA01Bound:2x_CAAnalogue:GLC-GLCUnboundUnboundUnboundUnboundUnbound
3cgtA01Bound:2x_CAUnboundUnboundBound:BCDUnboundUnboundUnbound
4cgtA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
5cgtA01Bound:2x_CAAnalogue:GLC-GLCUnboundUnboundBound:GLC 711UnboundUnbound
6cgtA01Bound:2x_CAAnalogue:OPG-DAG-GLC-OPG-DAG-GLCUnboundUnboundUnboundUnboundUnbound
7cgtA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
8cgtA01Bound:2x_CAAnalogue:TM6UnboundUnboundUnboundUnboundUnbound
9cgtA01Bound:2x_CAAnalogue:TM5UnboundUnboundUnboundUnboundUnbound
1cdgA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1cgvA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1cgwA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1cgxA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1cgyA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1cxeA01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLCUnboundUnboundUnboundUnboundUnbound
1cxfA01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLCUnboundUnboundUnboundUnboundUnbound
1cxgA01Bound:2x_CAAnalogue:ACRUnboundUnboundUnboundUnboundUnbound
1cxhA01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLCUnboundUnboundUnboundUnboundUnbound
1cxiA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1cxkA01Bound:2x_CABound:GLC-GLC-GLC-GLC-GLC-GLC-GLC-GLC-GLC(chain C)UnboundUnboundUnboundUnboundUnbound
1cxlA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundIntermediate-bound:GLC-GLC-G4D
1d3cA01Bound:2x_CAUnboundUnboundBound:GLC-GLC-GLC-GLC-GLC-GLC-GLC-GLC(chain C)UnboundUnboundUnbound
1dijA01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLC-GLC-GLC-ACI-GLC-GLCUnboundUnboundUnboundUnboundUnbound
1dtuA01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-GLC-GLC-ADH-G6D-GLC(chain C)Unbound
1eo5A01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLC-GLC-GLC-GLC(chain B)UnboundUnboundUnboundUnboundUnbound
1eo7A01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLC-GLC-GLC(chain B)UnboundUnboundUnboundUnboundUnbound
1kckA01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-ADH-G6D-GLC(chain B)Unbound
1kclA01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLC(chain B)UnboundUnboundUnboundUnboundUnbound
1ot1A01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLC-GLC-GLC-GLC(chain C)UnboundUnboundUnboundUnboundUnbound
1ot2A01Bound:2x_CAAnalogue:GLC-GLC-GLC-GLC-GLC-GLC-GLC(chain C)UnboundUnboundUnboundUnboundUnbound
1pezA01Bound:2x_CAAnalogue:GLC-GLC(chain C)UnboundUnboundUnboundUnboundUnbound
1pj9A01Bound:2x_CAAnalogue:GLC-GLC(chain C)UnboundUnboundBound:GLC-GLC(chain B)UnboundUnbound
1tcmA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1tcmB01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
2cxgA01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-ACI-G6D-GLCUnbound
2dijA01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GLC-GLC-GLC-GLC-GLC-GLC-ADH-G6D-GLCUnbound
1cygA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1a47A01Bound:2x_CAUnboundUnboundUnboundUnboundTransition-state-analogue:GTE-GLC-GLC-ACI-G6D-GLC-GLCUnbound
1ciuA01Bound:2x_CAUnboundUnboundUnboundUnboundUnboundUnbound
1d7fA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1d7fB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dedA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dedB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1i75A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1i75B02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pamA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pamB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ukqA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ukqB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uksA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uksB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uktA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uktB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3jA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3jB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3kA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3kB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3lA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3lB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3mA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3mB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgtA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cguA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3cgtA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
4cgtA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
5cgtA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
6cgtA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
7cgtA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
8cgtA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
9cgtA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cdgA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgvA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgwA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgxA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgyA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxeA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxfA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxgA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxhA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxiA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxkA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxlA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1d3cA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dijA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dtuA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1eo5A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1eo7A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1kckA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1kclA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ot1A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ot2A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pezA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pj9A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1tcmA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1tcmB02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2cxgA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2dijA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cygA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1a47A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ciuA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1d7fA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1d7fB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dedA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dedB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1i75A03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1i75B03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pamA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pamB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ukqA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ukqB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uksA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uksB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uktA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uktB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3jA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3jB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3kA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3kB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3lA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3lB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3mA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3mB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgtA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cguA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3cgtA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
4cgtA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
5cgtA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
6cgtA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
7cgtA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
8cgtA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
9cgtA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cdgA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgvA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgwA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgxA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgyA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxeA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxfA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxgA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxhA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxiA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxkA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxlA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1d3cA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dijA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dtuA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1eo5A03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1eo7A03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1kckA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1kclA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ot1A03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ot2A03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pezA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pj9A03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1tcmA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1tcmB03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2cxgA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2dijA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cygA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1a47A03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ciuA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1d7fA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1d7fB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dedA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dedB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1i75A04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1i75B04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pamA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pamB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ukqA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ukqB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uksA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uksB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uktA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1uktB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3jA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3jB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3kA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3kB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3lA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3lB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3mA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1v3mB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgtA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cguA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3cgtA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
4cgtA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
5cgtA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
6cgtA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
7cgtA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
8cgtA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
9cgtA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cdgA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgvA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgwA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgxA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cgyA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxeA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxfA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxgA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxhA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxiA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxkA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cxlA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1d3cA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dijA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dtuA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1eo5A04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1eo7A04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1kckA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1kclA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ot1A04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ot2A04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pezA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1pj9A04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1tcmA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1tcmB04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2cxgA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2dijA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1cygA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1a47A04UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1ciuA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound

Active-site residues
resource
Swiss-prot;P05618, P30920, P43379, P31797, P26827 & literature [8], [11], [16]
pdbCatalytic residuesCofactor-binding residuescomment
1d7fA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;       (Calcium-2)
mutant H233N
1d7fB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;       (Calcium-2)
mutant H233N
1dedA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;       (Calcium-2)
mutant H233N
1dedB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;       (Calcium-2)
mutant H233N
1i75A01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1i75B01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1pamA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1pamB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1ukqA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1ukqB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1uksA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F183L, F259L
1uksB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F183L, F259L
1uktA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y100L
1uktB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y100L
1v3jA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F283L
1v3jB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F283L
1v3kA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F283Y
1v3kB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F283Y
1v3lA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F283L
1v3lB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F283L
1v3mA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F283Y
1v3mB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant F283Y
1cgtA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1cguA01       ;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D229A
3cgtA01ASP 229;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant E257A
4cgtA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant deletion 145-150, F151D
5cgtA01       ;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D229A
6cgtA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant L194T
7cgtA01       ;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D229A
8cgtA01ASP 229;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant E257A
9cgtA01ASP 229;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant E257A
1cdgA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1cgvA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y195F
1cgwA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y195G
1cgxA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y195L
1cgyA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y195W
1cxeA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1cxfA01       ;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D229N, E257Q
1cxgA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1cxhA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1cxiA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

1cxkA01       ;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D229N, E257Q
1cxlA01ASP 229;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant E257Q
1d3cA01       ;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D229N, E257Q
1dijA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y195F
1dtuA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y89D, S146P
1eo5A01       ;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D229A, E257A
1eo7A01       ;       ;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D229A, E257A
1kckA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant N193G
1kclA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant G179L
1ot1A01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D135A
1ot2A01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant D135N
1pezA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant A230V
1pj9A01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant T185S, T186Y, N188D, K192R
1tcmA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant W616A
1tcmB01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant W616A
2cxgA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)

2dijA01ASP 229;GLU 257;HIS 327;ASP 328
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 139;ILE 190;ASP 199;HIS 233(Calcium-2)
mutant Y195F
1cygA01ASP 225;GLU 253;HIS 323;ASP 324
ASP 24;ASN 26;ASN 29;ASN 30;GLY 48;ASP 50(Calcium-1);ASN 135;ILE 186;ASP 195;HIS 229(Calcium-2)

1a47A01ASP 230;GLU 258;HIS 328;ASP 329
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 140;ILE 191;ASP 200;HIS 234(Calcium-2)

1ciuA01ASP 230;GLU 258;HIS 328;ASP 329
ASP 27;ASN 29;ASN 32;ASN 33;GLY 51;ASP 53(Calcium-1);ASN 140;ILE 191;ASP 200;HIS 234(Calcium-2)

1d7fA02                       


1d7fB02                       


1dedA02                       


1dedB02                       


1i75A02                       


1i75B02                       


1pamA02                       


1pamB02                       


1ukqA02                       


1ukqB02                       


1uksA02                       


1uksB02                       


1uktA02                       


1uktB02                       


1v3jA02                       


1v3jB02                       


1v3kA02                       


1v3kB02                       


1v3lA02                       


1v3lB02                       


1v3mA02                       


1v3mB02                       


1cgtA02                       


1cguA02                       


3cgtA02                       


4cgtA02                       


5cgtA02                       


6cgtA02                       


7cgtA02                       


8cgtA02                       


9cgtA02                       


1cdgA02                       


1cgvA02                       


1cgwA02                       


1cgxA02                       


1cgyA02                       


1cxeA02                       


1cxfA02                       


1cxgA02                       


1cxhA02                       


1cxiA02                       


1cxkA02                       


1cxlA02                       


1d3cA02                       


1dijA02                       


1dtuA02                       


1eo5A02                       


1eo7A02                       


1kckA02                       


1kclA02                       


1ot1A02                       


1ot2A02                       


1pezA02                       


1pj9A02                       


1tcmA02                       


1tcmB02                       


2cxgA02                       


2dijA02                       


1cygA02                       


1a47A02                       


1ciuA02                       


1d7fA03                       


1d7fB03                       


1dedA03                       


1dedB03                       


1i75A03                       


1i75B03                       


1pamA03                       


1pamB03                       


1ukqA03                       


1ukqB03                       


1uksA03                       


1uksB03                       


1uktA03                       


1uktB03                       


1v3jA03                       


1v3jB03                       


1v3kA03                       


1v3kB03                       


1v3lA03                       


1v3lB03                       


1v3mA03                       


1v3mB03                       


1cgtA03                       


1cguA03                       


3cgtA03                       


4cgtA03                       


5cgtA03                       


6cgtA03                       


7cgtA03                       


8cgtA03                       


9cgtA03                       


1cdgA03                       


1cgvA03                       


1cgwA03                       


1cgxA03                       


1cgyA03                       


1cxeA03                       


1cxfA03                       


1cxgA03                       


1cxhA03                       


1cxiA03                       


1cxkA03                       


1cxlA03                       


1d3cA03                       


1dijA03                       


1dtuA03                       


1eo5A03                       


1eo7A03                       


1kckA03                       


1kclA03                       


1ot1A03                       


1ot2A03                       


1pezA03                       


1pj9A03                       


1tcmA03                       


1tcmB03                       


2cxgA03                       


2dijA03                       


1cygA03                       


1a47A03                       


1ciuA03                       


1d7fA04                       


1d7fB04                       


1dedA04                       


1dedB04                       


1i75A04                       


1i75B04                       


1pamA04                       


1pamB04                       


1ukqA04                       


1ukqB04                       


1uksA04                       


1uksB04                       


1uktA04                       


1uktB04                       


1v3jA04                       


1v3jB04                       


1v3kA04                       


1v3kB04                       


1v3lA04                       


1v3lB04                       


1v3mA04                       


1v3mB04                       


1cgtA04                       


1cguA04                       


3cgtA04                       


4cgtA04                       


5cgtA04                       


6cgtA04                       


7cgtA04                       


8cgtA04                       


9cgtA04                       


1cdgA04                       


1cgvA04                       


1cgwA04                       


1cgxA04                       


1cgyA04                       


1cxeA04                       


1cxfA04                       


1cxgA04                       


1cxhA04                       


1cxiA04                       


1cxkA04                       


1cxlA04                       


1d3cA04                       


1dijA04                       


1dtuA04                       


1eo5A04                       


1eo7A04                       


1kckA04                       


1kclA04                       


1ot1A04                       


1ot2A04                       


1pezA04                       


1pj9A04                       


1tcmA04                       


1tcmB04                       


2cxgA04                       


2dijA04                       


1cygA04                       


1a47A04                       


1ciuA04                       



References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[11]Fig.6, p.9935
[12]p.70
[15]p.598
[20]p.2238-2240
[22]p.1143-1145
[23]p.5913-5914
[31]p.5578-5579
[34]Fig.1
[35]Fig.2, p.407-408
[36]Fig.1, p.432-433
[38]Fig.1, Fig.6
[39]Fig.4, p.342
[57]Scheme 1

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[8]
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Medline ID91171298
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JournalJ Mol Biol
Year1991
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Related PDB1cgt
Related Swiss-protP30920
[9]
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[10]
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Year1992
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Related PDB1cgu
[11]
PubMed ID8061001
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Year1994
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Pages9929-36
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[12]
PubMed ID8276116
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Year1994
Volume337
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[13]
PubMed ID7764720
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Year1994
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[14]
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Year1994
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[15]
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PubMed ID8107143
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Year1994
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[16]
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Year1994
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Volume61
Pages1257-65
AuthorsWind RD, Liebl W, Buitelaar RM, Penninga D, Spreinat A, Dijkhuizen L, Bahl H
TitleCyclodextrin formation by the thermostable alpha-amylase of Thermoanaerobacterium thermosulfurigenes EM1 and reclassification of the enzyme as a cyclodextrin glycosyltransferase.
[18]
PubMed ID7832788
JournalBiochem J
Year1995
Volume305
Pages685-6
AuthorsJanecek S, MacGregor EA, Svensson B
TitleCharacteristic differences in the primary structure allow discrimination of cyclodextrin glucanotransferases from alpha-amylases.
[19]
CommentsX-ray crystallography
PubMed ID7880832
JournalBiochemistry
Year1995
Volume34
Pages3368-76
AuthorsPenninga D, Strokopytov B, Rozeboom HJ, Lawson CL, Dijkstra BW, Bergsma J, Dijkhuizen L
TitleSite-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity.
Related PDB1cgv,1cgw,1cgx,1cgy
[20]
CommentsX-ray crystallography
PubMed ID7857935
JournalBiochemistry
Year1995
Volume34
Pages2234-40
AuthorsStrokopytov B, Penninga D, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW. X-ray structure of cyclodextrin glycosyltransferase complexed with acarbose
TitleImplications for the catalytic mechanism of glycosidases.
Related PDB1cxg,2cxg
[21]
CommentsX-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
Medline ID96094317
PubMed ID7493956
JournalJ Biol Chem
Year1995
Volume270
Pages29256-64
AuthorsKnegtel RM, Strokopytov B, Penninga D, Faber OG, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW
TitleCrystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products.
Related PDB1cxe,1cxf,1cxh,1cxi
Related Swiss-protP43379
[22]
CommentsX-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
JournalActa Crystallogr D Biol Crystallogr
Year1996
Volume52
Pages1136-45
AuthorsHarata K, Haga K, Nakamura A, Aoyagi M, Yamane K
TitleX-ray structure of cyclodextrin glucanotransferase from alkalophilic Bacillus Sp. 1011. Comparison of two independent molecules at 1.8-A resolution.
Related PDB1pam
Related Swiss-protP05618
[23]
CommentsX-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF COMPLEX WITH INHIBITOR.
Medline ID96264806
PubMed ID8672460
JournalBiochemistry
Year1996
Volume35
Pages4241-9
AuthorsStrokopytov B, Knegtel RM, Penninga D, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW
TitleStructure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity.
Related PDB1dij,2dij
Related Swiss-protP43379
[24]
CommentsX-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
Medline ID97115811
PubMed ID8955113
JournalJ Biol Chem
Year1996
Volume271
Pages32777-84
AuthorsPenninga D, van der Veen BA, Knegtel RM, van Hijum SA, Rozeboom HJ, Kalk KH, Dijkstra BW, Dijkhuizen L
TitleThe raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251.
Related PDB1tcm
Related Swiss-protP43379
[25]
CommentsX-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
Medline ID96177766
PubMed ID8604143
JournalJ Mol Biol
Year1996
Volume256
Pages611-22
AuthorsKnegtel RM, Wind RD, Rozeboom HJ, Kalk KH, Buitelaar RM, Dijkhuizen L, Dijkstra BW
TitleCrystal structure at 2.3 A resolution and revised nucleotide sequence of the thermostable cyclodextrin glycosyltransferase from Thermonanaerobacterium thermosulfurigenes EM1.
Related PDB1ciu
Related Swiss-protP26827
[26]
PubMed ID9401418
JournalProg Biophys Mol Biol
Year1997
Volume67
Pages67-97
AuthorsJanecek S
Titlealpha-Amylase family: molecular biology and evolution.
[27]
PubMed ID9860832
JournalBiochemistry
Year1998
Volume37
Pages17192-8
AuthorsMosi R, Sham H, Uitdehaag JC, Ruiterkamp R, Dijkstra BW, Withers SG
TitleReassessment of acarbose as a transition state analogue inhibitor of cyclodextrin glycosyltransferase.
[28]
CommentsX-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
Medline ID98226626
PubMed ID9558324
JournalBiochemistry
Year1998
Volume37
Pages5909-15
AuthorsSchmidt AK, Cottaz S, Driguez H, Schulz GE
TitleStructure of cyclodextrin glycosyltransferase complexed with a derivative of its main product beta-cyclodextrin.
Related PDB3cgt
Related Swiss-protP30920
[29]
CommentsX-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
Medline ID98409292
PubMed ID9738912
JournalEur J Biochem
Year1998
Volume255
Pages710-7
AuthorsParsiegla G, Schmidt AK, Schulz GE
TitleSubstrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production.
Related PDB4cgt,5cgt,6cgt,7cgt,8cgt,9cgt
Related Swiss-protP30920
[30]
PubMed ID9654055
JournalEur J Biochem
Year1998
Volume253
Pages598-605
AuthorsWind RD, Buitelaar RM, Dijkhuizen L
TitleEngineering of factors determining alpha-amylase and cyclodextrin glycosyltransferase specificity in the cyclodextrin glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1.
[31]
CommentsX-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS).
Medline ID98157977
PubMed ID9488711
JournalJ Biol Chem
Year1998
Volume273
Pages5771-9
AuthorsWind RD, Uitdehaag JC, Buitelaar RM, Dijkstra BW, Dijkhuizen L
TitleEngineering of cyclodextrin product specificity and pH optima of the thermostable cyclodextrin glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1.
Related PDB1a47
Related Swiss-protP26827
[32]
PubMed ID9828462
JournalJ Biotechnol
Year1998
Volume65
Pages191-202
AuthorsChang HY, Irwin PM, Nikolov ZL
TitleEffects of mutations in the starch-binding domain of Bacillus macerans cyclodextrin glycosyltransferase.
[33]
PubMed ID10094484
JournalFEBS Lett
Year1999
Volume445
Pages333-7
AuthorsAlcalde M, Plou FJ, Andersen C, Martin MT, Pedersen S, Ballesteros A
TitleChemical modification of lysine side chains of cyclodextrin glycosyltransferase from Thermoanaerobacter causes a shift from cyclodextrin glycosyltransferase to alpha-amylase specificity.
[34]
CommentsX-ray crystallography
PubMed ID10574960
JournalJ Biol Chem
Year1999
Volume274
Pages34868-76
AuthorsUitdehaag JC, Kalk KH, van Der Veen BA, Dijkhuizen L, Dijkstra BW
TitleThe cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution.
Related PDB1d3c
[35]
PubMed ID10331861
JournalNat Struct Biol
Year1999
Volume6
Pages406-8
AuthorsDavies GJ, Wilson KS
TitleTrapped in the act of catalysis.
[36]
CommentsX-ray crystallography
PubMed ID10331869
JournalNat Struct Biol
Year1999
Volume6
Pages432-6
AuthorsUitdehaag JC, Mosi R, Kalk KH, van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW
TitleX-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.
Related PDB1cxk,1cxl
[37]
PubMed ID10877806
JournalAppl Environ Microbiol
Year2000
Volume66
Pages3058-64
AuthorsOhdan K, Kuriki T, Takata H, Kaneko H, Okada S
TitleIntroduction of raw starch-binding domains into Bacillus subtilis alpha-amylase by fusion with the starch-binding domain of Bacillus cyclomaltodextrin glucanotransferase.
[38]
CommentsX-ray crystallography
PubMed ID10869182
JournalBiochemistry
Year2000
Volume39
Pages7772-80
AuthorsUitdehaag JC, van Alebeek GJ, van Der Veen BA, Dijkhuizen L, Dijkstra BW
TitleStructures of maltohexaose and maltoheptaose bound at the donor sites of cyclodextrin glycosyltransferase give insight into the mechanisms of transglycosylation activity and cyclodextrin size specificity.
Related PDB1eo5,1eo7
[39]
PubMed ID11150613
JournalBiochim Biophys Acta
Year2000
Volume1543
Pages336-360
Authorsvan der Veen BA, Uitdehaag JC, Dijkstra BW, Dijkhuizen L
TitleEngineering of cyclodextrin glycosyltransferase reaction and product specificity.
[40]
PubMed ID10651801
JournalEur J Biochem
Year2000
Volume267
Pages658-65
Authorsvan der Veen BA, van Alebeek GJ, Uitdehaag JC, Dijkstra BW, Dijkhuizen L
TitleThe three transglycosylation reactions catalyzed by cyclodextrin glycosyltransferase from Bacillus circulans (strain 251) proceed via different kinetic mechanisms.
[41]
CommentsX-ray crystallography
PubMed ID10731709
JournalJ Biochem (Tokyo)
Year2000
Volume127
Pages383-91
AuthorsIshii N, Haga K, Yamane K, Harata K
TitleCrystal structure of alkalophilic asparagine 233-replaced cyclodextrin glucanotransferase complexed with an inhibitor, acarbose, at 2.0 A resolution.
Related PDB1ded
[42]
CommentsX-ray crystallography
PubMed ID10686101
JournalJ Mol Biol
Year2000
Volume296
Pages1027-38
Authorsvan der Veen BA, Uitdehaag JC, Penninga D, van Alebeek GJ, Smith LM, Dijkstra BW, Dijkhuizen L
TitleRational design of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 to increase alpha-cyclodextrin production.
Related PDB1dtu
[43]
CommentsX-ray crystallography
PubMed ID10679895
JournalJ Mol Recognit
Year2000
Volume13
Pages35-43
AuthorsIshii N, Haga K, Yamane K, Harata K
TitleCrystal structure of asparagine 233-replaced cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011 determined at 1.9 A resolution.
Related PDB1d7f
[44]
PubMed ID11675025
JournalCarbohydr Res
Year2001
Volume336
Pages47-53
AuthorsLee SB, Robyt JF
TitleTrapping of a covalent enzyme intermediate in the reaction of Bacillus macerans cyclomaltodextrin glucanyltransferase with cyclomaltohexaose.
[45]
CommentsX-ray crystallography
PubMed ID11275559
JournalJ Biochem (Tokyo)
Year2001
Volume129
Pages593-8
AuthorsKanai R, Haga K, Yamane K, Harata K
TitleCrystal structure of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011 complexed with 1-deoxynojirimycin at 2.0 A resolution.
Related PDB1i75
[46]
PubMed ID11555657
JournalJ Biol Chem
Year2001
Volume276
Pages44557-62
Authorsvan der Veen BA, Leemhuis H, Kralj S, Uitdehaag JC, Dijkstra BW, Dijkhuizen L
TitleHydrophobic amino acid residues in the acceptor binding site are main determinants for reaction mechanism and specificity of cyclodextrin-glycosyltransferase.
[47]
PubMed ID11288183
JournalProteins
Year2001
Volume43
Pages327-35
AuthorsUitdehaag JC, van der Veen BA, Dijkhuizen L, Elber R, Dijkstra BW
TitleEnzymatic circularization of a malto-octaose linear chain studied by stochastic reaction path calculations on cyclodextrin glycosyltransferase.
[48]
PubMed ID11943149
JournalFEBS Lett
Year2002
Volume514
Pages189-92
AuthorsLeemhuis H, Dijkstra BW, Dijkhuizen L
TitleMutations converting cyclodextrin glycosyltransferase from a transglycosylase into a starch hydrolase.
[49]
PubMed ID11879012
JournalJ Agric Food Chem
Year2002
Volume50
Pages1411-5
AuthorsLee SH, Kim YW, Lee S, Auh JH, Yoo SS, Kim TJ, Kim JW, Kim ST, Rho HJ, Choi JH, Kim YB, Park KH
TitleModulation of cyclizing activity and thermostability of cyclodextrin glucanotransferase and its application as an antistaling enzyme.
[50]
PubMed ID11790748
JournalJ Bacteriol
Year2002
Volume184
Pages777-84
AuthorsRashid N, Cornista J, Ezaki S, Fukui T, Atomi H, Imanaka T
TitleCharacterization of an archaeal cyclodextrin glucanotransferase with a novel C-terminal domain.
[51]
CommentsX-ray crystallography
PubMed ID11696539
JournalJ Biol Chem
Year2002
Volume277
Pages1113-9
AuthorsLeemhuis H, Uitdehaag JC, Rozeboom HJ, Dijkstra BW, Dijkhuizen L
TitleThe remote substrate binding subsite -6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanism.
Related PDB1kck,1kcl
[52]
PubMed ID12554949
JournalActa Crystallogr D Biol Crystallogr
Year2003
Volume59
Pages348-9
AuthorsChoe HW, Park KS, Labahn J, Granzin J, Kim CJ, Buldt G
TitleCrystallization and preliminary X-ray diffraction studies of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans.
[53]
CommentsX-ray crystallography
PubMed ID12809508
JournalBiochemistry
Year2003
Volume42
Pages7518-26
AuthorsLeemhuis H, Rozeboom HJ, Wilbrink M, Euverink GJ, Dijkstra BW, Dijkhuizen L
TitleConversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: the role of alanine 230 in acceptor subsite +1.
Related PDB1pez
[54]
CommentsX-ray crystallography
PubMed ID12706817
JournalFEBS Lett
Year2003
Volume541
Pages47-51
AuthorsLeemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L
TitleThe fully conserved Asp residue in conserved sequence region I of the alpha-amylase family is crucial for the catalytic site architecture and activity.
Related PDB1ot1,1ot2
[55]
CommentsX-ray crystallography
PubMed ID14769878
JournalJ Biochem (Tokyo)
Year2003
Volume134
Pages881-91
AuthorsHaga K, Kanai R, Sakamoto O, Aoyagi M, Harata K, Yamane K
TitleEffects of essential carbohydrate/aromatic stacking interaction with Tyr100 and Phe259 on substrate binding of cyclodextrin glycosyltransferase from alkalophilic Bacillus sp. 1011.
Related PDB1ukq,1uks,1ukt
[56]
PubMed ID12890607
JournalJ Biotechnol
Year2003
Volume103
Pages203-12
AuthorsLeemhuis H, Kragh KM, Dijkstra BW, Dijkhuizen L
TitleEngineering cyclodextrin glycosyltransferase into a starch hydrolase with a high exo-specificity.
[57]
PubMed ID15023061
JournalBiochemistry
Year2004
Volume43
Pages3104-10
AuthorsJensen MH, Mirza O, Albenne C, Remaud-Simeon M, Monsan P, Gajhede M, Skov LK
TitleCrystal structure of the covalent intermediate of amylosucrase from Neisseria polysaccharea.
[58]
PubMed ID15476414
JournalBiochemistry
Year2004
Volume43
Pages13204-13
AuthorsLeemhuis H, Wehmeier UF, Dijkhuizen L
TitleSingle amino acid mutations interchange the reaction specificities of cyclodextrin glycosyltransferase and the acarbose-modifying enzyme acarviosyl transferase.
[59]
PubMed ID15096580
JournalProtein Eng Des Sel
Year2004
Volume17
Pages205-11
AuthorsShim JH, Kim YW, Kim TJ, Chae HY, Park JH, Cha H, Kim JW, Kim YR, Schaefer T, Spendler T, Moon TW, Park KH
TitleImprovement of cyclodextrin glucanotransferase as an antistaling enzyme by error-prone PCR.
[60]
CommentsX-ray crystallography
PubMed ID14739329
JournalProtein Sci
Year2004
Volume13
Pages457-65
AuthorsKanai R, Haga K, Akiba T, Yamane K, Harata K
TitleRole of Phe283 in enzymatic reaction of cyclodextrin glycosyltransferase from alkalophilic Bacillus sp.1011: Substrate binding and arrangement of the catalytic site.
Related PDB1v3j,1v3k,1v3l,1v3m
[61]
CommentsX-ray crystallography
PubMed ID14705029
JournalProteins
Year2004
Volume54
Pages128-34
AuthorsLeemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L
TitleImproved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge.
Related PDB1pj9

comments
This enzyme belongs to the glycosidase family-13 (or family of alpha-amylase).
Although this enzyme binds two calcium ions on the catalytic domain, they are not involved in catalytic reaction.
According to the literature [20], [31], [34], [35], [36] & [39], the reaction proceeds as follows:
(1) Asp328 (and/or His327) modulate(s) the activity of Glu257 by raising its pKa, so that the carboxylate of Glu257 is protonated to act as a general acid.
(2) Glu257 acts as a general acid, to protonate the leaving O4 atom, leading to the formation of oxocarbonium transition-state (SN1-like reaction). This transition state is stabilized by Asp328 and His327.
(3) Asp229 acts as a nucleophile, attacking the C1 atom to form a covalent bond with it, which is an intermediate.
(4) The acceptor group, O4 hydroxyl group of the non-reducing end, approaches the active site.
(5) Glu257 acts as a general base to deprotonate the acceptor, O4 hydroxyl group.
(6) The O4 hydroxyl group makes a nucleophilic attack on the C1 atom, leading to the formation of oxocarbonium transition-state (SN1-like reaction). This transition state is stabilized by Asp328 and His327.
(7) Finally, Asp229 is releaased.

createdupdated
2005-10-052011-12-01
Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2010)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)

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