EzCatDB: S00017

DB codeS00017
CATH domainDomain 11.10.489.10Catalytic domain
E.C.1.11.1.10
CSA2cpo
MACiEM0250


Enzyme Name
Swiss-protKEGG

P04963
Protein nameChloroperoxidasechloride peroxidase
chloroperoxidase
SynonymsEC 1.11.1.10
Chloride peroxidase
CPO


Swiss-prot:Accession NumberP04963
Entry namePRXC_CALFU
ActivityRH + Cl(-) + H(2)O(2) = RCl + 2 H(2)O.
Subunit
Subcellular location
CofactorBinds 1 heme B (iron-protoporphyrin IX) group per subunit.,Binds 1 manganese ion per subunit.


CofactorsSubstratesProducts
KEGG-idC00032C00034C00027C00698C00708C01324C01371C00001C00720C01321C01334
CompoundHemeManganeseH2O2Cl-I-Br-RHH2ORBrRIRCl
Typearomatic ring (with nitrogen atoms),carboxyl group,heavy metalheavy metalothershalidehalidehalidelipidH2Ohalidehalidehalide
1cpoABound:HEMBound:_MNUnboundUnboundUnboundUnboundUnboundBound:HOH 801UnboundUnboundUnbound
2cpoABound:HEMBound:_MNUnboundUnboundUnboundUnboundUnboundBound:HOH 801UnboundUnboundUnbound

Active-site residues
resource
Swiss-prot;P04963
pdbCatalytic residuesCofactor-binding residues
1cpoAHIS 105;GLU 183
CYS 29(Fe binding);GLU 104;HIS 105;SER 108(Manganese binding)
2cpoAHIS 105;GLU 183
CYS 29(Fe binding);GLU 104;HIS 105;SER 108(Manganese binding)

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[4]p.1370-1374
[5]Fig.1, Fig.2, Fig.6, Fig.86
[10]Scheme 1p.670

references
[1]
PubMed ID7200984
JournalJ Biol Chem
Year1982
Volume257
Pages7768-9
AuthorsRubin B, VanMiddlesworth J, Thomas K, Hager L
TitleCrystallization and preliminary X-ray data for chloroperoxidase.
[2]
CommentsDETERMINATION OF HEME LIGAND
Medline ID89066662
PubMed ID3198598
JournalJ Biol Chem
Year1988
Volume263
Pages18739-43
AuthorsBlanke SR, Hager LP
TitleIdentification of the fifth axial heme ligand of chloroperoxidase.
Related Swiss-protP04963
[3]
PubMed ID8188677
JournalJ Biol Chem
Year1994
Volume269
Pages13966-78
AuthorsHosten CM, Sullivan AM, Palaniappan V, Fitzgerald MM, Terner J
TitleResonance Raman spectroscopy of the catalytic intermediates and derivatives of chloroperoxidase from Caldariomyces fumago.
[4]
CommentsX-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)
Medline ID96363674
PubMed ID8747463
JournalStructure
Year1995
Volume3
Pages1367-77
AuthorsSundaramoorthy M, Terner J, Poulos TL
TitleThe crystal structure of chloroperoxidase: a heme peroxidase--cytochrome P450 functional hybrid.
Related PDB1cpo,2cpo
Related Swiss-protP04963
[5]
PubMed ID9195874
JournalChem Biol
Year1997
Volume4
Pages367-72
AuthorsWagenknecht HA, Woggon WD
TitleIdentification of intermediates in the catalytic cycle of chloroperoxidase.
[6]
PubMed ID9671545
JournalChem Res Toxicol
Year1998
Volume11
Pages816-23
AuthorsToy PH, Newcomb M, Hager LP
TitleHypersensitive radical probe studies of chloroperoxidase-catalyzed hydroxylation reactions.
[7]
CommentsHomologous enzyme
PubMed ID9551547
JournalStructure
Year1998
Volume6
Pages255-8
AuthorsPoulos TL, Raman CS, Li H
TitleNO news is good news.
[8]
CommentsMUTAGENESIS OF CYS-50
Medline ID20006248
PubMed ID10535936
JournalProc Natl Acad Sci U S A
Year1999
Volume96
Pages12412-7
AuthorsYi X, Mroczko M, Manoj KM, Wang X, Hager LP
TitleReplacement of the proximal heme thiolate ligand in chloroperoxidase with a histidine residue.
Related Swiss-protP04963
[9]
PubMed ID11460242
JournalBiotechnol Bioeng
Year2001
Volume72
Pages523-9
Authorsvan de Velde F, Bakker M, van Rantwijk F, Sheldon RA
TitleChloroperoxidase-catalyzed enantioselective oxidations in hydrophobic organic media.
[10]
PubMed ID11168405
JournalEur J Biochem
Year2001
Volume268
Pages665-72
AuthorsBaciocchi E, Fabbrini M, Lanzalunga O, Manduchi L, Pochetti G
TitleProchiral selectivity in H(2)O(2)-promoted oxidation of arylalkanols catalysed by chloroperoxidase. The role of the interactions between the OH group and the amino-acid residues in the enzyme active site.
[11]
PubMed ID11278701
JournalJ Biol Chem
Year2001
Volume276
Pages17635-40
AuthorsConesa A, van De Velde F, van Rantwijk F, Sheldon RA, van Den Hondel CA, Punt PJ
TitleExpression of the Caldariomyces fumago chloroperoxidase in Aspergillus niger and characterization of the recombinant enzyme.
[12]
PubMed ID11684191
JournalPhytochemistry
Year2001
Volume58
Pages929-33
AuthorsVazquez-Duhalt R, Ayala M, Marquez-Rocha FJ
TitleBiocatalytic chlorination of aromatic hydrocarbons by chloroperoxidase of Caldariomyces fumago.
[13]
PubMed ID12127969
JournalBiochem Biophys Res Commun
Year2002
Volume295
Pages828-31
AuthorsAyala M, Horjales E, Pickard MA, Vazquez-Duhalt R
TitleCross-linked crystals of chloroperoxidase.


createdupdated
2004-01-262009-02-26
Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2010)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)

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