EzCatDB: S00026

DB codeS00026
CATH domainDomain 11.10.600.10Catalytic domain
E.C.4.2.3.9
CSA1di1
MACiEM0261

CATH domainRelated DB codes (homologues)
1.10.600.10S00024,S00027

Enzyme Name
Swiss-protKEGG

Q03471
Protein nameAristolochene synthasearistolochene synthase
sesquiterpene cyclase
trans,trans-farnesyl diphosphate aristolochene-lyase
trans,trans-farnesyl-diphosphate diphosphate-lyase (cyclizing,aristolochene-forming)
SynonymsAS
EC 4.2.3.9
Sesquiterpene cyclase

KEGG pathways
MAP codePathways
MAP00900Terpenoid biosynthesis

Swiss-prot:Accession NumberQ03471
Entry nameARIS_PENRO
Activity2-trans,6-trans-farnesyl diphosphate = aristolochene + diphosphate.
Subunit
Subcellular locationCytoplasm.
CofactorMagnesium.,Manganese.


CofactorsSubstratesProducts
KEGG-idC00305C00034C00448C02004C00013
CompoundMagnesiumManganesetrans,trans-farnesyl diphosphateAristolochenediphosphate
Typedivalent metal (Ca2+, Mg2+)heavy metallipid,phosphate group/phosphate ionlipidphosphate group/phosphate ion
1dgpAUnboundUnboundAnalogue:FOHUnboundUnbound
1dgpBUnboundUnboundAnalogue:FOHUnboundUnbound
1di1AUnboundUnboundUnboundUnboundUnbound
1di1BUnboundUnboundUnboundUnboundUnbound

Active-site residues
resource
literature [5]
pdbCatalytic residues
1dgpATYR 92
1dgpBTYR 92
1di1ATYR 92
1di1BTYR 92

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[1]SCHEME I
[4]Fig.5, Fig.63
[5]Scheme 1p.7742

references
[1]
PubMed ID7986100
JournalArch Biochem Biophys
Year1994
Volume315
Pages527-32
AuthorsBack K, Yin S, Chappell J
TitleExpression of a plant sesquiterpene cyclase gene in Escherichia coli.
[2]
PubMed ID8563643
JournalProtein Sci
Year1995
Volume4
Pages2436-8
AuthorsLesburg CA, Lloyd MD, Cane DE, Christianson DW
TitleCrystallization and preliminary X-ray diffraction analysis of recombinant pentalenene synthase.
[3]
PubMed ID9324768
JournalScience
Year1997
Volume277
Pages1788-9
AuthorsSacchettini JC, Poulter CD
TitleCreating isoprenoid diversity.
[4]
CommentsX-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
Medline ID20400487
PubMed ID10825154
JournalJ Biol Chem
Year2000
Volume275
Pages25533-9
AuthorsCaruthers JM, Kang I, Rynkiewicz MJ, Cane DE, Christianson DW
TitleCrystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti.
Related PDB1dgp,1di1
Related Swiss-protQ03471
[5]
PubMed ID12820883
JournalBiochemistry
Year2003
Volume42
Pages7741-7
AuthorsDeligeorgopoulou A, Allemann RK
TitleEvidence for differential folding of farnesyl pyrophosphate in the active site of aristolochene synthase: a single-point mutation converts aristolochene synthase into an (E)-beta-farnesene synthase.


createdupdated
2004-05-242009-02-26


Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2010)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)

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