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| Enzyme Name | | Swiss-prot | KEGG |
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| P0C1A8 | P83218 |
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| Protein name | Pectinesterase A | Pectinesterase | pectinesterasepectin demethoxylasepectin methoxylasepectin methylesterasepectasepectin methyl esterasepectinoesterase |
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| Synonyms | PE AEC 3.1.1.11Pectin methylesterase A | PEEC 3.1.1.11Pectin methylesterase |
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| KEGG pathways | | MAP code | Pathways |
|---|
| MAP00040 | Pentose and glucuronate interconversions | | MAP00500 | Starch and sucrose metabolism |
| Swiss-prot:Accession Number | P0C1A8 | P83218 |
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| Entry name | PMEA_ERWCH | PME_DAUCA |
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| Activity | Pectin + n H(2)O = n methanol + pectate. | Pectin + n H(2)O = n methanol + pectate. |
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| Subunit | Monomer (By similarity). |
|
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| Subcellular location | Secreted (By similarity). | Secreted, cell wall (Probable). |
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| Cofactor |
|
|
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| Substrates | Products |
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| KEGG-id | C00714 | C00001 | C00132 | C00470 |
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| Compound | Pectin | H2O | Methanol | Pectate |
|---|
| Type | carbohydrate,polysaccharide | H2O | carbohydrate | carboxyl group,polysaccharide |
|---|
| 1qjvA |  | Unbound |
| Unbound | Unbound |
|---|
| 1qjvB | | Unbound |
| Unbound | Unbound |
|---|
| 1gq8A | | Unbound |
| Unbound | Unbound |
|---|
| References for Catalytic Mechanism | | References | Sections | No. of steps in catalysis |
|---|
| [2] | Scheme 1, p.347-349 |
| | [8] | p.955-957 |
| | [11] | p.248 |
|
| references | | [1] |
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| PubMed ID | 2837615 |
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| Journal | Mol Microbiol |
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| Year | 1988 |
|---|
| Volume | 2 |
|---|
| Pages | 247-54 |
|---|
| Authors | Plastow GS |
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| Title | Molecular cloning and nucleotide sequence of the pectin methyl esterase gene of Erwinia chrysanthemi B374. |
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| [2] |
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| PubMed ID | 1953633 |
|---|
| Journal | Biochem J |
|---|
| Year | 1991 |
|---|
| Volume | 279 |
|---|
| Pages | 343-50 |
|---|
| Authors | Nari J, Noat G, Ricard J |
|---|
| Title | Pectin methylesterase, metal ions and plant cell-wall extension. Hydrolysis of pectin by plant cell-wall pectin methylesterase. |
|---|
| [3] |
|---|
| PubMed ID | 1868195 |
|---|
| Journal | Plant Mol Biol |
|---|
| Year | 1991 |
|---|
| Volume | 16 |
|---|
| Pages | 501-13 |
|---|
| Authors | Albani D, Altosaar I, Arnison PG, Fabijanski SF |
|---|
| Title | A gene showing sequence similarity to pectin esterase is specifically expressed in developing pollen of Brassica napus. Sequences in its 5' flanking region are conserved in other pollen-specific promoters. |
|---|
| [4] |
|---|
| PubMed ID | 8370537 |
|---|
| Journal | Gene |
|---|
| Year | 1993 |
|---|
| Volume | 131 |
|---|
| Pages | 17-25 |
|---|
| Authors | Laurent F, Kotoujansky A, Labesse G, Bertheau Y |
|---|
| Title | Characterization and overexpression of the pem gene encoding pectin methylesterase of Erwinia chrysanthemi strain 3937. |
|---|
| [5] |
|---|
| PubMed ID | 10564001 |
|---|
| Journal | J Agric Food Chem |
|---|
| Year | 1999 |
|---|
| Volume | 47 |
|---|
| Pages | 1471-5 |
|---|
| Authors | Sun D, Wicker L |
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| Title | Kinetic compensation and the role of cations in pectinesterase catalysis. |
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| [6] |
|---|
| PubMed ID | 10956097 |
|---|
| Journal | J Agric Food Chem |
|---|
| Year | 2000 |
|---|
| Volume | 48 |
|---|
| Pages | 3238-44 |
|---|
| Authors | Corredig M, Wicker L |
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| Title | Role of cations in the catalysis of thermostable pectinmethylesterase extracted from Marsh grapefruit pulp. |
|---|
| [7] |
|---|
| PubMed ID | 11120736 |
|---|
| Journal | J Biol Chem |
|---|
| Year | 2001 |
|---|
| Volume | 276 |
|---|
| Pages | 8841-7 |
|---|
| Authors | Goldberg R, Pierron M, Bordenave M, Breton C, Morvan C, du Penhoat CH |
|---|
| Title | Control of Mung bean pectinmethylesterase isoform activities. Influence of pH and carboxyl group distribution along the pectic chains. |
|---|
| [8] |
|---|
| Comments | X-ray crystallography |
|---|
| PubMed ID | 11162105 |
|---|
| Journal | J Mol Biol |
|---|
| Year | 2001 |
|---|
| Volume | 305 |
|---|
| Pages | 951-60 |
|---|
| Authors | Jenkins J, Mayans O, Smith D, Worboys K, Pickersgill RW |
|---|
| Title | Three-dimensional structure of Erwinia chrysanthemi pectin methylesterase reveals a novel esterase active site. |
|---|
| Related PDB | 1qjv |
|---|
| [9] |
|---|
| PubMed ID | 11544130 |
|---|
| Journal | Trends Plant Sci |
|---|
| Year | 2001 |
|---|
| Volume | 6 |
|---|
| Pages | 414-9 |
|---|
| Authors | Micheli F |
|---|
| Title | Pectin methylesterases: cell wall enzymes with important roles in plant physiology. |
|---|
| [10] |
|---|
| PubMed ID | 11934288 |
|---|
| Journal | Biotechnol Prog |
|---|
| Year | 2002 |
|---|
| Volume | 18 |
|---|
| Pages | 221-8 |
|---|
| Authors | Terefe NS, Hendrickx M |
|---|
| Title | Kinetics of the pectin methylesterase catalyzed de-esterification of pectin in frozen food model systems. |
|---|
| [11] |
|---|
| PubMed ID | 11943159 |
|---|
| Journal | FEBS Lett |
|---|
| Year | 2002 |
|---|
| Volume | 514 |
|---|
| Pages | 243-9 |
|---|
| Authors | Johansson K, El-Ahmad M, Friemann R, Jornvall H, Markovic O, Eklund H |
|---|
| Title | Crystal structure of plant pectin methylesterase. |
|---|
| Related PDB | 1gq8 |
|---|
| comments | According to the literature [11], the catalytic reaction proceeds as follwos:
(1) Arg225 (of PDB;1gq8) enhances the nucleophilicity of Asp157 by its interaction.
(2) Asp157 makes a nucleophilic attack on the carbonyl carbon, resulting in the covalent bond formation leading to a negatively charged tetrahedral intermediate. Here, Asp136 acts as a general acid to protonate the leaving group, methanol.
(3) Gln113 and Gln135 stabilize the negative charge of the tetrahedral intermediate.
(4) Asp136 acts as a general base to activate a water molecule, which would attack on the tetrahedral intermediate, to complete the reaction.
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| created | updated |
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| 2004-03-22 | 2009-02-26 |
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