EzCatDB: T00018

DB codeT00018
CATH domainDomain 13.10.120.10Catalytic domain
Domain 23.90.420.10Catalytic domain
Domain 32.60.40.650
E.C.1.8.3.1
CSA1sox
MACiEM0121

CATH domainRelated DB codes (homologues)
3.10.120.10D00014

Enzyme Name
Swiss-protKEGG

P07850P51687
Protein nameSulfite oxidaseSulfite oxidase, mitochondrialsulfite oxidase
SynonymsEC 1.8.3.1
EC 1.8.3.1

KEGG pathways
MAP codePathways
MAP00920Sulfur metabolism

Swiss-prot:Accession NumberP07850P51687
Entry nameSUOX_CHICKSUOX_HUMAN
ActivitySulfite + O(2) + H(2)O = sulfate + H(2)O(2).Sulfite + O(2) + H(2)O = sulfate + H(2)O(2).
SubunitHomodimer.Homodimer (By similarity).
Subcellular locationMitochondrion intermembrane space.Mitochondrion intermembrane space.
CofactorBinds 1 protoheme group.,Molybdenum (molybdopterin).Binds 1 protoheme group.,Molybdenum (molybdopterin).


CofactorsSubstratesProducts
KEGG-idC00150C05924C00032C00094C00007C00001C00059C00027
CompoundMolybdenumMolybdopterinHemeSulfiteO2H2OSulfateH2O2
Typeheavy metalamide group,amine group,aromatic ring (with nitrogen atoms),carbohydrate,phosphate group/phosphate ion,sulfhydryl grouparomatic ring (with nitrogen atoms),carboxyl group,heavy metalsulfiteothersH2Osulfate groupothers
1soxA1UnboundUnboundBound:HEMUnboundUnbound
UnboundUnbound
1soxB1UnboundUnboundBound:HEMUnboundUnbound
UnboundUnbound
1mj4AUnboundUnboundBound:HEMUnboundUnbound
UnboundUnbound
1soxA2Bound:_MO-MTEBound:MTE-_MOUnboundUnboundUnbound
Bound:SO4 503Unbound
1soxB2Bound:_MO-MTEBound:MTE-_MOUnboundUnboundUnbound
Bound:SO4 503Unbound
1soxA3UnboundUnboundUnboundUnboundUnbound
UnboundUnbound
1soxB3UnboundUnboundUnboundUnboundUnbound
UnboundUnbound

Active-site residues
resource
Swiss-prot;P07850
pdbCatalytic residuesCofactor-binding residues
1soxA1
HIS 40;HIS 65(Heme iron binding)
1soxB1
HIS 40;HIS 65(Heme iron binding)
1mj4A
HIS 40;HIS 65(Heme iron binding)
1soxA2TYR 322
CYS 185(Molybdenum binding)
1soxB2TYR 322
CYS 185(Molybdenum binding)
1soxA3

1soxB3


References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[12]p.976-981
[14]Scheme 1, p.6672-6676
[15]Fig.1, Fig.2, Fig.8, Fig.9
[19]Scheme 1
[20]Scheme 2, p.69
[26]Scheme 1
[28]p.363-366
[29]

[30]Fig.2
[31]Scheme 1
[32]Fig.1, Fig.2
[35]Fig.7, p.15112-15113

references
[1]
PubMed ID6258584
JournalBiochem J
Year1980
Volume191
Pages285-8
AuthorsGutteridge S, Lamy MT, Bray RC
TitleThe nature of the phosphate inhibitor complex of sulphite oxidase from electron-paramagnetic-resonance studies using oxygen-17.
[2]
PubMed ID6249254
JournalBiochem J
Year1980
Volume185
Pages397-403
AuthorsLamy MT, Gutteridge S, Bary RC
TitleElectron-paramagnetic-resonance parameters of molybdenum(V) in sulphite oxidase from chicken liver.
[3]
PubMed ID6244290
JournalJ Biol Chem
Year1980
Volume255
Pages2694-9
AuthorsCoughlan MP, Johnson JL, Rajagopalan KV
TitleMechanisms of inactivation of molybdoenzymes by cyanide.
[4]
PubMed ID6282260
JournalBiochem J
Year1982
Volume201
Pages241-3
AuthorsBray RC, Lamy MT, Gutteridge S, Wilkinson T
TitleEvidence from electron-paramagnetic-resonance spectroscopy for a complex of sulphite ions with the molybdenum centre of sulphite oxidase.
[5]
PubMed ID6307274
JournalBiochem J
Year1983
Volume211
Pages227-36
AuthorsBray RC, Gutteridge S, Lamy MT, Wilkinson T
TitleEquilibria amongst different molybdenum (V)-containing species from sulphite oxidase. Evidence for a halide ligand of molybdenum in the low-pH species.
[6]
PubMed ID6546929
JournalJ Biol Chem
Year1984
Volume259
Pages5414-22
AuthorsJohnson JL, Hainline BE, Rajagopalan KV, Arison BH
TitleThe pterin component of the molybdenum cofactor. Structural characterization of two fluorescent derivatives.
[7]
PubMed ID3806088
JournalJ Inorg Biochem
Year1986
Volume28
Pages107-23
AuthorsMitchell PC
TitleMolybdenum in enzymatic and heterogeneous catalysis.
[8]
PubMed ID3680253
JournalJ Biol Chem
Year1987
Volume262
Pages16357-63
AuthorsKramer SP, Johnson JL, Ribeiro AA, Millington DS, Rajagopalan KV
TitleThe structure of the molybdenum cofactor. Characterization of di-(carboxamidomethyl)molybdopterin from sulfite oxidase and xanthine oxidase.
[9]
PubMed ID2851985
JournalBiochem J
Year1988
Volume256
Pages307-9
AuthorsGeorge GN, Prince RC, Kipke CA, Sunde RA, Enemark JH
TitleThe nature of the phosphate complex of sulphite oxidase from electron-paramagnetic-resonance studies.
[10]
PubMed ID2548601
JournalBiochemistry
Year1989
Volume28
Pages5075-80
AuthorsGeorge GN, Kipke CA, Prince RC, Sunde RA, Enemark JH, Cramer SP
TitleStructure of the active site of sulfite oxidase. X-ray absorption spectroscopy of the Mo(IV), Mo(V), and Mo(VI) oxidation states.
[11]
PubMed ID8190702
JournalPharmacol Toxicol
Year1994
Volume74
Pages136-40
AuthorsConstantin D, Mehrotra K, Jernstrom B, Tomasi A, Moldeus P
TitleAlternative pathways of sulfite oxidation in human polymorphonuclear leukocytes.
[12]
CommentsX-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), AND SEQUENCE REVISION TO 152.
Medline ID98088796
PubMed ID9428520
JournalCell
Year1997
Volume91
Pages973-83
AuthorsKisker C, Schindelin H, Pacheco A, Wehbi WA, Garrett RM, Rajagopalan KV, Enemark JH, Rees DC
TitleMolecular basis of sulfite oxidase deficiency from the structure of sulfite oxidase.
Related PDB1sox
Related Swiss-protP07850
[13]
PubMed ID9652170
JournalProg Biophys Mol Biol
Year1997
Volume68
Pages121-44
AuthorsRomao MJ, Knablein J, Huber R, Moura JJ
TitleStructure and function of molybdopterin containing enzymes.
[14]
PubMed ID10350486
JournalBiochemistry
Year1999
Volume38
Pages6668-77
AuthorsBrody MS, Hille R
TitleThe kinetic behavior of chicken liver sulfite oxidase.
[15]
PubMed ID10555573
JournalJ Biol Inorg Chem
Year1999
Volume4
Pages390-401
AuthorsPacheco A, Hazzard JT, Tollin G, Enemark JH
TitleThe pH dependence of intramolecular electron transfer rates in sulfite oxidase at high and low anion concentrations.
[16]
PubMed ID11185564
JournalArch Biochem Biophys
Year2000
Volume383
Pages281-7
AuthorsTemple CA, Graf TN, Rajagopalan KV
TitleOptimization of expression of human sulfite oxidase and its molybdenum domain.
[17]
PubMed ID11196982
JournalInorg Chem
Year2000
Volume39
Pages4989-92
AuthorsAstashkin AV, Cosper MM, Raitsimring AM, Enemark JH
TitleESEEM and mims ENDOR spectroscopies of cis,trans-(L-N2S2)MoVO(SCH2Ph): detection of the two benzylthiolate alpha protons.
[18]
PubMed ID11229572
JournalInorg Chem
Year2000
Volume39
Pages525-31
AuthorsMader ML, Carducci MD, Enemark JH
TitleAnalogues for the molybdenum center of sulfite oxidase: oxomolybdenum(V) complexes with three thiolate sulfur donor atoms.
[19]
PubMed ID11151370
JournalInorg Chem
Year2000
Volume39
Pages5697-706
AuthorsMcNaughton RL, Tipton AA, Rubie ND, Conry RR, Kirk ML
TitleElectronic structure studies of oxomolybdenum tetrathiolate complexes: origin of reduction potential differences and relationship to cysteine-molybdenum bonding in sulfite oxidase.
[20]
PubMed ID10830849
JournalJ Inorg Biochem
Year2000
Volume79
Pages67-74
AuthorsThapper A, Lorber C, Fryxelius J, Behrens A, Nordlander E
TitleSynthesis and reactivity studies of model complexes for molybdopterin-dependent enzymes.
[21]
PubMed ID11516283
JournalJ Am Chem Soc
Year2001
Volume123
Pages8343-9
AuthorsLim BS, Willer MW, Miao M, Holm RH
TitleMonodithiolene molybdenum(V, VI) complexes: a structural analogue of the oxidized active site of the sulfite oxidase enzyme family.
[22]
PubMed ID11598126
JournalJ Biol Chem
Year2001
Volume276
Pages46989-94
AuthorsEilers T, Schwarz G, Brinkmann H, Witt C, Richter T, Nieder J, Koch B, Hille R, Hansch R, Mendel RR
TitleIdentification and biochemical characterization of Arabidopsis thaliana sulfite oxidase. A new player in plant sulfur metabolism.
[23]
PubMed ID11980485
JournalBiochemistry
Year2002
Volume41
Pages5816-21
AuthorsFeng C, Kedia RV, Hazzard JT, Hurley JK, Tollin G, Enemark JH
TitleEffect of solution viscosity on intramolecular electron transfer in sulfite oxidase.
[24]
PubMed ID12022845
JournalJ Am Chem Soc
Year2002
Volume124
Pages6109-18
AuthorsAstashkin AV, Raitsimring AM, Feng C, Johnson JL, Rajagopalan KV, Enemark JH
TitlePulsed EPR studies of nonexchangeable protons near the Mo(V) center of sulfite oxidase: direct detection of the alpha-proton of the coordinated cysteinyl residue and structural implications for the active site.
[25]
PubMed ID12296723
JournalJ Am Chem Soc
Year2002
Volume124
Pages11612-3
AuthorsElliott SJ, McElhaney AE, Feng C, Enemark JH, Armstrong FA
TitleA voltammetric study of interdomain electron transfer within sulfite oxidase.
[26]
PubMed ID12148977
JournalJ Am Chem Soc
Year2002
Volume124
Pages9006-7
AuthorsPeariso K, McNaughton RL, Kirk ML
TitleActive-site stereochemical control of oxygen atom transfer reactivity in sulfite oxidase.
[27]
PubMed ID11935358
JournalJ Biol Inorg Chem
Year2002
Volume7
Pages338-50
AuthorsCodd R, Astashkin AV, Pacheco A, Raitsimring AM, Enemark JH
TitlePulsed ELDOR spectroscopy of the Mo(V)/Fe(III) state of sulfite oxidase prepared by one-electron reduction with Ti(III) citrate.
[28]
PubMed ID12114025
JournalTrends Biochem Sci
Year2002
Volume27
Pages360-7
AuthorsHille R
TitleMolybdenum and tungsten in biology.
[29]
CommentsX-ray crystallography
PubMed ID12832761
JournalActa Crystallogr D Biol Crystallogr
Year2003
Volume59
Pages1183-91
AuthorsRudolph MJ, Johnson JL, Rajagopalan KV, Kisker C
TitleThe 1.2 A structure of the human sulfite oxidase cytochrome b(5) domain.
Related PDB1mj4
[30]
PubMed ID12950200
JournalInorg Chem
Year2003
Volume42
Pages5531-6
AuthorsJalilehvand F, Lim BS, Holm RH, Hedman B, Hodgson KO
TitleX-ray absorption spectroscopy of a structural analogue of the oxidized active sites in the sulfite oxidase enzyme family and related molybdenum(V) complexes.
[31]
PubMed ID14514295
JournalInorg Chem
Year2003
Volume42
Pages6194-203
AuthorsPeariso K, Chohan BS, Carrano CJ, Kirk ML
TitleSynthesis and EPR characterization of new models for the one-electron reduced molybdenum site of sulfite oxidase.
[32]
PubMed ID12424234
JournalJ Biol Chem
Year2003
Volume278
Pages2913-20
AuthorsFeng C, Wilson HL, Hurley JK, Hazzard JT, Tollin G, Rajagopalan KV, Enemark JH
TitleRole of conserved tyrosine 343 in intramolecular electron transfer in human sulfite oxidase.
[33]
PubMed ID14527393
JournalStructure (Camb)
Year2003
Volume11
Pages1251-63
AuthorsSchrader N, Fischer K, Theis K, Mendel RR, Schwarz G, Kisker C
TitleThe crystal structure of plant sulfite oxidase provides insights into sulfite oxidation in plants and animals.
Related PDB1ogp
[34]
PubMed ID14989655
JournalInorg Chem
Year2004
Volume43
Pages1625-37
AuthorsMcNaughton RL, Helton ME, Cosper MM, Enemark JH, Kirk ML
TitleNature of the oxomolybdenum-thiolate pi-bond: implications for Mo-S bonding in sulfite oxidase and xanthine oxidase.
[35]
PubMed ID14729666
JournalJ Biol Chem
Year2004
Volume279
Pages15105-13
AuthorsWilson HL, Rajagopalan KV
TitleThe role of tyrosine 343 in substrate binding and catalysis by human sulfite oxidase.

comments
This enzyme is composed of Molybdenum (Mo) catalytic domain and Heme-Fe catalytic domain. It catalyzes the following reactions during the catalytic cycle (see [15]):
(A) Two-electron oxidation of SO3(2-) to SO4(2-) at the Mo(VI) catalytic site.
(B) Intramolecular electron transfer (IET) from the Mo(IV) catalytic site to the Heme-Fe(III) catalytic site.
(C) One-electron transfer from the Heme-Fe(II) catalytic site to exogenous (cyt c)ox.
(D) IET from the Mo(V) catalytic site to the Heme-Fe(III) catalytic site.
(E) One-electron transfer from the Heme-Fe(II) catalytic site to exogenous (cyt c)ox.

createdupdated
2004-12-272009-02-26
Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/JST (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005-)

© Computational Biology Research Center, AIST, 2004 All Rights Reserved.