EzCatDB: T00253

DB codeT00253
RLCP classification1.12.30000.10
CATH domainDomain 13.40.50.1820Catalytic domain
Domain 22.60.60.20
Domain 32.10.80.10
E.C.3.1.1.3
CSA1hpl
MACiEM0218

CATH domainRelated DB codes (homologues)
2.60.60.20M00041
3.40.50.1820S00544,S00344,S00517,S00525,S00526,S00720,S00723,S00724,S00725,S00919,S00057,S00374,S00345,S00347,S00348,S00346,S00350,S00352,S00353,S00355,S00356,S00358,D00189,D00210,D00539

Enzyme Name
Swiss-protKEGG

P81139P54318P00591P29183P06857P16233P17084P02703P02704P02705P19090P04118
Protein namePancreatic lipase-related protein 2Pancreatic lipase-related protein 2Pancreatic triacylglycerol lipasePancreatic triacylglycerol lipasePancreatic lipase-related protein 1Pancreatic triacylglycerol lipaseColipaseColipaseColipase AColipase BColipaseColipasetriacylglycerol lipase
lipase
triglyceride lipase
tributyrase
butyrinase
glycerol ester hydrolase
tributyrinase
Tween hydrolase
steapsin
triacetinase
tributyrin esterase
Tweenase
amno N-AP
Takedo 1969-4-9
Meito MY 30
Tweenesterase
GA 56
capalase L
triglyceride hydrolase
triolein hydrolase
tween-hydrolyzing esterase
amano CE
cacordase
triglyceridase
triacylglycerol ester hydrolase
amano P
amano AP
PPL
glycerol-ester hydrolase
GEH
meito Sangyo OF lipase
hepatic lipase
lipazin
post-heparin plasma protamine-resistant lipase
salt-resistant post-heparin lipase
heparin releasable hepatic lipase
amano CES
amano B
tributyrase
triglyceride lipase
liver lipase
hepatic monoacylglycerol acyltransferase
SynonymsPL-RP2
EC 3.1.1.3
GPL
PL-RP2
EC 3.1.1.3
Secretory glycoprotein GP-3
Pancreatic lipase
PL
EC 3.1.1.3
Pancreatic lipase
PL
EC 3.1.1.3
PL-RP1
EC 3.1.1.3
Pancreatic lipase
PL
EC 3.1.1.3
NoneProcolipase II
NoneNoneNoneNone

KEGG pathways
MAP codePathways
MAP00561Glycerolipid metabolism

Swiss-prot:Accession NumberP81139P54318P00591P29183P06857P16233P17084P02703P02704P02705P19090P04118
Entry nameLIPR2_CAVPOLIPR2_RATLIPP_PIGLIPP_HORSELIPR1_CANFALIPP_HUMANCOL_RATCOL_PIGCOLA_HORSECOLB_HORSECOL_CANFACOL_HUMAN
ActivityTriacylglycerol + H(2)O = diacylglycerol + a carboxylate.Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.





Subunit





Forms a 1:1 stoichiometric complex with pancreatic lipase (By similarity).Forms a 1:1 stoichiometric complex with pancreatic lipase.Forms a 1:1 stoichiometric complex with pancreatic lipase.Forms a 1:1 stoichiometric complex with pancreatic lipase.Forms a 1:1 stoichiometric complex with pancreatic lipase.Forms a 1:1 stoichiometric complex with pancreatic lipase.
Subcellular locationSecreted.Secreted. Membrane.Secreted.Secreted.Secreted.Secreted.Secreted.Secreted.Secreted.Secreted.Secreted.Secreted.
Cofactor













CofactorsSubstratesProductsintermediates
KEGG-idC00076C00422C00001C00060C00162C00165I00123I00085I00086
CompoundCalciumTriacylglycerolH2OCarboxylateFatty acidDiacylglycerolPeptidyl-Ser-tetrahedral intermediate (with previous carboxylic-ester)Acyl-enzyme(Peptidyl-Ser-acyl group)Peptidyl-Ser-tetrahedral-intermediate
Typedivalent metal (Ca2+, Mg2+)carbohydrate,lipidH2Ocarboxyl groupfatty acidcarbohydrate,lipid


1gplA01Bound:_CAUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1bu8A01UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1ethA01Bound:_CAUnbound
UnboundUnboundAnalogue:C8E-C8EUnboundUnboundUnbound
1ethC01Bound:_CAUnbound
UnboundUnboundAnalogue:C8E-C8EUnboundUnboundUnbound
1hplA01Bound:_CAUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1hplB01Bound:_CAUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1rp1A01UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1lpaB01Bound:_CAAnalogue:PLC
UnboundUnboundUnboundUnboundUnboundUnbound
1lpbB01Bound:_CAUnbound
UnboundUnboundUnboundTransition-state-analogue:MUPUnboundUnbound
1n8sA01UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1gplA02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1bu8A02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1ethA02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1ethC02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1hplA02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1hplB02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1rp1A02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1lpaB02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1lpbB02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1n8sA02UnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1ethBUnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1ethDUnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1lpaAUnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1lpbAUnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1n8sCUnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1pcnAUnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound
1pcoAUnboundUnbound
UnboundUnboundUnboundUnboundUnboundUnbound

Active-site residues
resource
Swiss-prot;P00591, P06857, P16233, P29183, P54318 & literature [11]
pdbCatalytic residuesCofactor-binding residuesMain-chain involved in catalysis
1gplA01SER 152;ASP 176;HIS 263
GLU 187;ARG 190;ASP 192;ASP 195(Calcium binding)
PHE 77;LEU 153
1bu8A01SER 152;ASP 176;HIS 263
GLU 187;ARG 190;ASP 192;ASP 195(Calcium binding)
PHE 77;LEU 153
1ethA01SER 153;ASP 177;HIS 264
GLU 188;ARG 191;ASP 193;ASP 196(Calcium binding)
PHE 78;LEU 154
1ethC01SER 153;ASP 177;HIS 264
GLU 188;ARG 191;ASP 193;ASP 196(Calcium binding)
PHE 78;LEU 154
1hplA01SER 152;ASP 176;HIS 263
GLU 187;ARG 190;ASP 192;ASP 195(Calcium binding)
PHE 77;LEU 153
1hplB01SER 152;ASP 176;HIS 263
GLU 187;ARG 190;ASP 192;ASP 195(Calcium binding)
PHE 77;LEU 153
1rp1A01SER 152;ASP 176;HIS 263
GLU 187;ARG 190;ASP 192;ASP 195(Calcium binding)
PHE 77;LEU 153
1lpaB01SER 152;ASP 176;HIS 263
GLU 187;ARG 190;ASP 192;ASP 195(Calcium binding)
PHE 77;LEU 153
1lpbB01SER 152;ASP 176;HIS 263
GLU 187;ARG 190;ASP 192;ASP 195(Calcium binding)
PHE 77;LEU 153
1n8sA01SER 152;ASP 176;HIS 263
GLU 187;ARG 190;ASP 192;ASP 195(Calcium binding)
PHE 77;LEU 153
1gplA02                       


1bu8A02                       


1ethA02                       


1ethC02                       


1hplA02                       


1hplB02                       


1rp1A02                       


1lpaB02                       


1lpbB02                       


1n8sA02                       


1ethB


1ethD


1lpaA


1lpbA


1n8sC


1pcnA


1pcoA



References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[2]

[6]Fig.7, p.99-103
[11]p.817
[14]p.720-722
[15]Fig.3, p.468
[17]


references
[1]
PubMed ID2322552
JournalBiochemistry
Year1990
Volume29
Pages1136-42
AuthorsDeckelbaum RJ, Hamilton JA, Moser A, Bengtsson-Olivecrona G, Butbul E, Carpentier YA, Gutman A, Olivecrona T
TitleMedium-chain versus long-chain triacylglycerol emulsion hydrolysis by lipoprotein lipase and hepatic lipase: implications for the mechanisms of lipase action.
[2]
CommentsX-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
Medline ID90158821
PubMed ID2106079
JournalNature
Year1990
Volume343
Pages771-4
AuthorsWinkler FK, D'Arcy A, Hunziker W
TitleStructure of human pancreatic lipase.
Related Swiss-protP16233
[3]
PubMed ID1940628
JournalJ Lipid Res
Year1991
Volume32
Pages963-75
AuthorsKomaromy MC, Reed M
TitleExpression of rat hepatic lipase in heterologous systems: evidence for different sites for interface binding and catalysis.
[4]
PubMed ID2046751
JournalNature
Year1991
Volume351
Pages491-4
AuthorsBrzozowski AM, Derewenda U, Derewenda ZS, Dodson GG, Lawson DM, Turkenburg JP, Bjorkling F, Huge-Jensen B, Patkar SA, Thim L
TitleA model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex.
[5]
PubMed ID1368796
JournalEnzyme Microb Technol
Year1992
Volume14
Pages426-46
AuthorsMalcata FX, Reyes HR, Garcia HS, Hill CG Jr, Amundson CH
TitleKinetics and mechanisms of reactions catalysed by immobilized lipases.
[6]
PubMed ID1290943
JournalFaraday Discuss
Year1992
Volume-93
Pages95-105
AuthorsDodson GG, Lawson DM, Winkler FK
TitleStructural and evolutionary relationships in lipase mechanism and activation.
[7]
PubMed ID1544468
JournalFEBS Lett
Year1992
Volume299
Pages111-5
AuthorsLuthi-Peng Q, Marki HP, Hadvary P
TitleIdentification of the active-site serine in human pancreatic lipase by chemical modification with tetrahydrolipstatin.
[8]
CommentsX-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
Medline ID92396238
PubMed ID1522902
JournalNature
Year1992
Volume359
Pages159-62
Authorsvan Tilbeurgh H, Sarda L, Verger R, Cambillau C
TitleStructure of the pancreatic lipase-procolipase complex.
Related Swiss-protP16233
[9]
PubMed ID8409770
JournalJ Lipid Res
Year1993
Volume34
Pages1393-40
AuthorsDichek HL, Parrott C, Ronan R, Brunzell JD, Brewer HB Jr, Santamarina-Fojo S
TitleFunctional characterization of a chimeric lipase genetically engineered from human lipoprotein lipase and human hepatic lipase.
[10]
PubMed ID8301235
JournalJ Lipid Res
Year1993
Volume34
Pages2169-76
AuthorsWolle J, Jansen H, Smith LC, Chan L
TitleFunctional role of N-linked glycosylation in human hepatic lipase: asparagine-56 is important for both enzyme activity and secretion.
[11]
CommentsX-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
Medline ID93241293
PubMed ID8479519
JournalNature
Year1993
Volume362
Pages814-20
Authorsvan Tilbeurgh H, Egloff MP, Martinez C, Rugani N, Verger R, Cambillau C
TitleInterfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography.
Related Swiss-protP16233
[12]
PubMed ID7918595
JournalBiochim Biophys Acta
Year1994
Volume1214
Pages148-60
Authorsvan Kuiken BA, Behnke WD
TitleThe activation of porcine pancreatic lipase by cis-unsaturated fatty acids.
[13]
PubMed ID7929247
JournalJ Biol Chem
Year1994
Volume269
Pages25470-4
AuthorsJennens ML, Lowe ME
TitleA surface loop covering the active site of human pancreatic lipase influences interfacial activation and lipid binding.
[14]
CommentsX-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)
Medline ID94238688
PubMed ID8182745
JournalJ Mol Biol
Year1994
Volume238
Pages709-32
AuthorsBourne Y, Martinez C, Kerfelec B, Lombardo D, Chapus C, Cambillau C
TitleHorse pancreatic lipase. The crystal structure refined at 2.3 A resolution.
Related PDB1hpl
Related Swiss-protP29183
[15]
PubMed ID7765546
JournalTrends Biotechnol
Year1994
Volume12
Pages464-72
AuthorsKazlauskas RJ
TitleElucidating structure-mechanism relationships in lipases: prospects for predicting and engineering catalytic properties.
[16]
PubMed ID7785848
JournalAnn N Y Acad Sci
Year1995
Volume750
Pages190-4
AuthorsVerger R, Ferrato F, Carriere F, Cudrey C, Rugani N, Gargouri Y, Hjorth A, Woldike H, Boel E, Thim L, et al
TitleRelationships between structures and kinetic properties of pancreatic lipases.
[17]
CommentsX-ray crystallography
PubMed ID7893686
JournalBiochemistry
Year1995
Volume34
Pages2751-62
AuthorsEgloff MP, Marguet F, Buono G, Verger R, Cambillau C, van Tilbeurgh H
TitleThe 2.46 A resolution structure of the pancreatic lipase-colipase complex inhibited by a C11 alkyl phosphonate.
Related PDB1lpb
[18]
PubMed ID9704093
JournalBiotechnol Annu Rev
Year1995
Volume1
Pages315-71
AuthorsAnthonsen HW, Baptista A, Drablos F, Martel P, Petersen SB, Sebastiao M, Vaz L
TitleLipases and esterases: a review of their sequences, structure and evolution.
[19]
PubMed ID7579978
JournalEXS
Year1995
Volume73
Pages3-26
AuthorsCarriere F, Verger R, Lookene A, Olivecrona G
TitleLipase structures at the interface between chemistry and biochemistry.
[20]
PubMed ID7533157
JournalJ Biol Chem
Year1995
Volume270
Pages3932-7
AuthorsAoubala M, de la Fourniere L, Douchet I, Abousalham A, Daniel C, Hirn M, Gargouri Y, Verger R, De Caro A
TitleHuman pancreatic lipase. Importance of the hinge region between the two domains, as revealed by monoclonal antibodies.
[21]
PubMed ID7658150
JournalJ Lipid Res
Year1995
Volume36
Pages1029-36
AuthorsJennens ML, Lowe ME
TitleC-terminal domain of human pancreatic lipase is required for stability and maximal activity but not colipase reactivation.
[22]
PubMed ID7773176
JournalProtein Sci
Year1995
Volume4
Pages44-57
AuthorsEgloff MP, Sarda L, Verger R, Cambillau C, van Tilbeurgh H
TitleCrystallographic study of the structure of colipase and of the interaction with pancreatic lipase.
[23]
CommentsX-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), AND REVISIONS TO 30-32
Medline ID96279347
PubMed ID8663362
JournalJ Biol Chem
Year1996
Volume271
Pages18007-16
AuthorsHermoso J, Pignol D, Kerfelec B, Crenon I, Chapus C, Fontecilla-Camps JC
TitleLipase activation by nonionic detergents. The crystal structure of the porcine lipase-colipase-tetraethylene glycol monooctyl ether complex.
Related PDB1eth
Related Swiss-protP00591
[24]
PubMed ID8939760
JournalStructure
Year1996
Volume4
Pages1363-74
AuthorsWithers-Martinez C, Carriere F, Verger R, Bourgeois D, Cambillau C
TitleA pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig.
[25]
CommentsX-ray crystallography
PubMed ID8993339
JournalBiochemistry
Year1997
Volume36
Pages239-48
AuthorsCarriere F, Thirstrup K, Hjorth S, Ferrato F, Nielsen PF, Withers-Martinez C, Cambillau C, Boel E, Thim L, Verger R
TitlePancreatic lipase structure-function relationships by domain exchange.
Related PDB1gpl
[26]
PubMed ID9312012
JournalEMBO J
Year1997
Volume16
Pages5531-6
AuthorsHermoso J, Pignol D, Penel S, Roth M, Chapus C, Fontecilla-Camps JC
TitleNeutron crystallographic evidence of lipase-colipase complex activation by a micelle.
[27]
PubMed ID9109604
JournalJ Nutr
Year1997
Volume127
Pages549-57
AuthorsLowe ME
TitleMolecular mechanisms of rat and human pancreatic triglyceride lipases.
[28]
PubMed ID9502110
JournalBioorg Med Chem
Year1998
Volume6
Pages109-18
AuthorsParmar VS, Pati HN, Azim A, Kumar R, Himanshu, Bisht KS, Prasad AK, Errington W
TitleLipase-catalysed selective deacetylation of phenolic/enolic acetoxy groups in peracetylated benzyl phenyl ketones.
[29]
PubMed ID9720253
JournalChem Phys Lipids
Year1998
Volume93
Pages103-14
AuthorsBezzine S, Roussel A, de Caro J, Gastinel L, de Caro A, Carriere F, Leydier S, Verger R, Cambillau C
TitleAn inactive pancreatic lipase-related protein is activated into a triglyceride-lipase by mutagenesis based on the 3-D structure.
[30]
PubMed ID9720254
JournalChem Phys Lipids
Year1998
Volume93
Pages123-9
AuthorsPignol D, Hermoso J, Kerfelec B, Crenon I, Chapus C, Fontecilla-Camps JC
TitleThe lipase/colipase complex is activated by a micelle: neutron crystallographic evidence.
[31]
PubMed ID9837944
JournalJ Biol Chem
Year1998
Volume273
Pages33604-9
AuthorsAyvazian L, Crenon I, Hermoso J, Pignol D, Chapus C, Kerfelec B
TitleIon pairing between lipase and colipase plays a critical role in catalysis.
[32]
CommentsX-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 17-468
Medline ID99041983
PubMed ID9822688
JournalJ Biol Chem
Year1998
Volume273
Pages32121-8
AuthorsRoussel A, Yang Y, Ferrato F, Verger R, Cambillau C, Lowe M
TitleStructure and activity of rat pancreatic lipase-related protein 2.
Related PDB1bu8
Related Swiss-protP54318
[33]
CommentsX-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)
Medline ID98393022
PubMed ID9726421
JournalProteins
Year1998
Volume32
Pages523-31
AuthorsRoussel A, de Caro J, Bezzine S, Gastinel L, de Caro A, Carriere F, Leydier S, Verger R, Cambillau C
TitleReactivation of the totally inactive pancreatic lipase RP1 by structure-predicted point mutations.
Related PDB1rp1
Related Swiss-protP06857
[34]
PubMed ID10501005
JournalBiosci Biotechnol Biochem
Year1999
Volume63
Pages1467-70
AuthorsTsuzuki W, Kitamura Y, Suzuki T, Mase T
TitleEffects of glucose on lipase activity.
[35]
PubMed ID10669782
JournalBiochim Biophys Acta
Year2000
Volume1476
Pages165-72
AuthorsMiled N, De Caro A, De Caro J, Verger R
TitleA conformational transition between an open and closed form of human pancreatic lipase revealed by a monoclonal antibody.
[36]
PubMed ID11099794
JournalBiochimie
Year2000
Volume82
Pages973-86
AuthorsMiled N, Canaan S, Dupuis L, Roussel A, Riviere M, Carriere F, de Caro A, Cambillau C, Verger R
TitleDigestive lipases: from three-dimensional structure to physiology.
[37]
PubMed ID12369922
JournalCurr Protein Pept Sci
Year2000
Volume1
Pages91-103
AuthorsChahinian H, Sias B, Carriere F
TitleThe C-terminal domain of pancreatic lipase: functional and structural analogies with c2 domains.
[38]
PubMed ID11377191
JournalBioorg Med Chem
Year2001
Volume9
Pages1345-8
AuthorsAzim A, Sharma SK, Olsen CE, Parmar VS
TitleLipase catalysed synthesis of optically enriched alpha-haloamides.
[39]
PubMed ID11557352
JournalBioorg Med Chem
Year2001
Volume9
Pages2643-52
AuthorsKumar R, Azim A, Kumar V, Sharma SK, Prasad AK, Howarth OW, Olsen CE, Jain SC, Parmar VS
TitleLipase-catalyzed chemo- and enantioselective acetylation of 2-alkyl/aryl-3-hydroxypropiophenones.
[40]
PubMed ID11212097
JournalBioorg Med Chem Lett
Year2001
Volume11
Pages305-7
AuthorsBasak A, Rudra KR, Bdour HM, Dasgupta J
TitleActive site geometry of porcine pancreatic lipase: an interesting switchover from Jones' to Seebach's model.
[41]
PubMed ID11514151
JournalBioorg Med Chem Lett
Year2001
Volume11
Pages2117-21
AuthorsMukherjee S, Prasad AK, Parmar VS, Howarth OW
TitleNovel lipase-catalysed enantioselective deacetylation of (+/-)-5-acetoxy-3-(4-fluorophenyl)-2-phenylisoxazolidine.
[42]
PubMed ID11457440
JournalChem Phys Lipids
Year2001
Volume111
Pages105-10
AuthorsCiuffreda P, Loseto A, Manzocchi A, Santaniello E
TitleLipolytic activity of porcine pancreas lipase on fatty acid esters of dialkylglycerols: a structural basis for the design of new substrates for the assay of pancreatic lipases activity.
[43]
PubMed ID11154696
JournalJ Biol Chem
Year2001
Volume276
Pages14014-8
AuthorsAyvazian L, Kerfelec B, Granon S, Foglizzo E, Crenon I, Dubois C, Chapus C
TitleThe lipase C-terminal domain. A novel unusual inhibitor of pancreatic lipase activity.
[44]
PubMed ID12022871
JournalBiochemistry
Year2002
Volume41
Pages6679-87
AuthorsAlam M, Vance DE, Lehner R
TitleStructure-function analysis of human triacylglycerol hydrolase by site-directed mutagenesis: identification of the catalytic triad and a glycosylation site.
[45]
PubMed ID12427035
JournalBiochemistry
Year2002
Volume41
Pages13725-35
AuthorsChahinian H, Bezzine S, Ferrato F, Ivanova MG, Perez B, Lowe ME, Carriere F
TitleThe beta 5' loop of the pancreatic lipase C2-like domain plays a critical role in the lipase-lipid interactions.
[46]
PubMed ID11836103
JournalBioorg Med Chem
Year2002
Volume10
Pages947-51
AuthorsPrasad AK, Himanshu, Bhattacharya A, Olsen CE, Parmar VS
TitleNovel lipase-catalysed highly selective acetylation studies on D-arabino- and D-threo-polyhydroxyalkyltriazoles.
[47]
PubMed ID12454260
JournalJ Lipid Res
Year2002
Volume43
Pages2007-16
AuthorsLowe ME
TitleThe triglyceride lipases of the pancreas.

comments
This enzyme forms a complex with a colipase protein, which is not involved in catalysis directly.
Although this enzyme binds a calcium ion, it is distant from the active site and not involved in catalysis.
This enzyme has got a catalytic triad composed of serine/histidine/aspartate in the N-terminal domain. The oxyanion hole seems to be made up by the mainchain amide groups of Phe77 and Leu153 (of PDB;1lpb). Thus, the catalytic mechanism must be similar to serine protease, trypsin.

createdupdated
2004-08-162012-10-22


Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2010)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)

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